BIOFILM FORMATION AND QUORUM SENSING ANALYSIS OF UROPATHOGENIC MULTIDRUG RESISTANCE ESCHERICHIA COLI
Objective: Escherichia coli (E. coli) are gram-negative facultative anaerobes which are commonly found in the lower intestine. Biofilm production in E. coli promotes colonization and leads to an increase rate of infections, and such infections may be difficult to treat as they exhibit multidrug resistance (MDR).
Methods: 50 strains of uropathogenic E. coli were collected from Clinical Microbiology laboratory at Saveetha medical college and hospital for a time period of 3 mo. Strains were identified by conventional biochemical methods. Biofilm formation and quorum sensing analysis were performed by the Microtitre plate method and Thin Layer Chromatography method (TLC), respectively.
Results: In this study, 46 (92%) of E. coli strains were strong, 3(6%) were intermediate and 1(2%) were weak biofilm producers. From TLC analysis, 34 (68%) of the strains produced Acyl Homoserine Lactone molecules. Out of which, 16 isolates were shown unknown analytes of Retardation factor (Rf) value greater than 1. The Rf values identified were 3 unsubstituted C4 (5), 3 unsubstituted C6 (3), 3 oxo C8 (3), 3 oxo C4 (4), 3 oxo C6 (2), 3 oxo C1 (1).
Conclusion: In this study, 100% of isolates were biofilm producers. Of which 18 strains produced known Acyl Homoserine Lactone molecules and 16 isolates produced unknown analytes. Thus, quorum sensing molecules plays a major role in biofilm formation.
2. Z Li, M Knetsch. Antibacterial strategies for wound dressing: preventing infection and stimulating healing. Curr Pharm Des 2018;24:936–51.
3. W Xu, S Dong, Y Han, S Li, Y Liu. Hydrogels as antibacterial biomaterials. Curr Pharm Design 2018;24:843–54.
4. Sevanan M, Pongiya U, Peedikavil NJ. Antimicrobial susceptibility pattern of biofilm-producing Escherichia coli of urinary tract infections. Curr Res Bacteriol 2011;4:73?80.
5. Suman E, Jose J, Varghese S, Kotian MS. Study of biofilm production in Escherichia coli causing urinary tract infection. Indian J Med Microbiol 2007;25:305?6.
6. Clinical and Laboratory Standards Institute (CLSI). Performance Standard-Ninth Edition (M2-A9). Wayne, PA: Clinical and Laboratoey Standards Institute; 2006.
7. Micheal L Vasil. DNA microarrays in an analysis of quorum sensing: strengths and limitation; 2003. p. 0021-9193.
8. Fiorela Nievas, Pablo Bogino, Fernando Sorroche, Walter Giordano. Detection characterization and biological effect of quorum sensing signaling molecules in peanut-nodulating bradyrhizobia. Sensors (Basel) 2012;12:2851–73.
9. Mette E Skindersoe, Mortan Alhede, Richard Phipps, Liang Yang, Peter O Jensen, Thomas B Rasmussen, et al. Effects of antibiotics on quorum sensing in Pseudomonas aeruginosa. Antimicrob Agents Chemother 2008;52:3648–63.
10. Nathaniel C Cady, Kurt A Mckean, Jason Behnke, Roman. Inhibition of biofilm formation, quorum sensing and infection in pseudomonas aeruginosa by naturalproducts–inspired organosulfur compounds. Plos One 2012;7:e38492.
11. Ponnusamy P, Natarajan V, Sevanan M. In vitro biofilm formation by pathogenic escherichia coli and their antimicrobial susceptibility pattern. Asian Pac J Trop Med 2012;5:210-3.
12. Tajbakhsh E, Tajbakhsh S, Khamesipour F. Isolation and molecular detection of gram-negative bacteria causing urinary tract infection in patients referred to shahrekord hoospitals. Iran Iran Red Crescent Med J 2015;17:e24779.
13. Mittal S, Sharma M, Chaudhary U. Biofilm and multidrug resistance in uropathogenic Escherichia coli. Pathog Glob Health 2015;109:26?9.
14. Tajbakhsh S, Khamesipour F. Isolation and molecular detection of gram negative bacteria causing urinary tract infection in patients referred to shahrekord hoospitals. Iran Iran Red Crescent Med J 2015;17:e24779.
This work is licensed under a Creative Commons Attribution 4.0 International License.