PHARMACOGNOSTICAL, PHYTOCHEMICAL ANALYSIS OF THE LEAVES OF PSIDIUM GUAJAVA LINNâ€“ LUCKNOW- 46 VARIEITY
Objective: To explore the micro morphology and physio chemical parameters of the leaves of Psidium guajava Linn. (Myrtaceae) â€“ Lucknow- 46 variety.
Methods: Macroscopy, microscopy, physicochemical analysis, preliminary phytochemical screening and other WHO recommended parameters for standardizations were performed.
Results: Leaves (5-15cm Ã— 4-6 cm) are dorsiventral, oblong â€“ elliptic, dull grey to yellow green with entire margin, obtuse to bluntly acuminate apex and rounded to subcuneate base with short petiole. Microscopic evaluation revealed the presence of paracytic stomata, three layers of wide rectangular cells, circular secretary cavities, calcium oxalate crystals, broadly bowl shaped vascular bundles, xylem vessels, phloem, and fibers.Â Â Â Â Â Â Â Â Â Â Â
Vein islet numbers, vein termination numbers, stomatal number, stomatal index and other physico chemical tests like ash values, loss on drying, extractive values were determined. Preliminary phytochemical screening showed the presence of sterols, tannins, proteins and aminoacids, flavonoids, volatile oil, terpenoids, saponin, carbohydrates and absence of alkaloids, mucilage, glycosides, fixed oil.
Conclusion: Microscopic analysis was informative and provides useful information in the botanical identification, standardization for purity & quality and immense value in authentication of the leaf.Â
2. Warrier PK, Nambiar VPK, Ramankutty C. Indian Medicinal Plants â€“ a Compendium of 500 Species. Vol. IV. Arya Vaidya Sala, Orient Longman Limited: Kottakal; 1995, 371.
3. Nadkarni KM, Nadkarni AK. Indian Materia Medica. Vol. I. 3rd ed, Popular Prakasham: Mumbai; 1976, 1019.
4. Nundkumar N, Ojewole JA. Studies on the antiplasmodial properties of some South African medicinal plants used as antimalarial remedies in Zulu folk medicine. Methods Find Exp Clin Pharmacol 2002; 24(7):397-401.
5. Hernandez, Dolores F. Plants of the Philippines. 2nd ed. M&L Licudine Enterprises: Phillipines; 1980.
6. Gnan SO, Demello MT. Inhibition of Staphylococcus aureus by aqueous Goiaba extracts. J Ethno pharmacol 1999; 68:103-8.
7. Oliver-Bever, Bep. Medicinal Plants in tropical West Africa. Cambridge University Press: Cambridge; 1986.
8. Jaiarj P, Khoohaswan P, Wongkrajang Y, Peungvicha P, Suriyawong P, Saraya MLS. Anticough and antimicrobial activities of Psidium guajava Linn. leaf extract. J Ethno pharmacol 1999; 67(2): 203-12.
9. Dutta BK, Rahman I, Das TK. In vitro study on antifungal property of common fruit plants. Biomedicine 2000; 20(3): 187-89.
10. Lin J. Puckree T. Mvelase TP. Anti-diarrhoeal evaluation of some medicinal plants used by Zulu traditional healers. J Ethno pharmacol 2002; 79(1): 53-6.
11. Ticzon, Romeo. Ticzon Herbal Medicine Encyclopaedia. Ticzon Publishing: Philippines; 1997.
12. Muruganandan S. Srinivasan K. Tandan SK. Jawahar Lal, Chandra S, Raviprakash V. Anti-inflammatory and analgesic activities of some medicinal plants. Journal of Medicinal and Aromatic Plant Sciences 2001; 22/23: 56-8.
13. Kavimani S. Karpagam RI. Jaykar B. Anti-inflammatory activity of volatile oil of Psidium guajava. Indian Journal of Pharmaceutical Sciences 1997; 59(3): 142-44.
14. Conway, Peter. Tree Medicine â€“ a comprehensive guide to the healing power of over 170 trees. Judy Piatkus (Publishers) Ltd: 2001.
15. Wyk, Ben-Erik van, Oudtshoorn, Bosch van, Gericke N. Medicinal Plants of South Africa. 1st ed. Briza Publications: Pretoria, South Africa; 1997.
16. Jain SK. Dictionary of Indian folk medicine and Ethnobotany. Deep Publications: New Delhi; 1991.
17. Qian H, Nihorimbere V. Antioxidant power of phytochemicals from Psidium guajava leaf. J Zheji Univ Sci 2004; 5(6): 676-83.
18. Ojewole JA. Hypoglycemic and hypotensive effects of Psidium guajava Linn leaf aqueous extract. Methods Find Exp Clin Pharmacol 2005; 27(10): 689-95.
19. Ojewole JA. Anti inflammatory and analgesic effects of Psidium guajava Linn leaf aqueous extract in rats and mice. Methods Find Exp Clin Pharmacol 2006; 28(7): 441-46.
20. Roy CK, Kamath JV, Asad M. Hepatoprotective activity of Psidium guajava Linn leaf extract. Indian J Exp Biol 2006; 44(4): 305-11.
21. Hsieh CL, Lin YC, Yen GC, Chen HY. Preventive effects of guava (Psidium guajava) leaves and its active compound against Î±-dicarbonyl compounds induced blood coagulation. Food chemistry 2007; 103(2): 528-35.
22. Lozoya X, Meckes M, Abouzaid M, Tortoriello J, Nozzolilloc, Arnason JT. Quercetin glycosides in Psidium guajava L leaves and determination of spasmolytic principle. Arch Med Res 1994; 25(1): 11-15.
23. Begum S, Hassan SI, Siddiqui BS, Shaheen F, Ghayur MN, Gilani AH. Triterpenoids from the leaves of Psidium guajava. Phytochemistry 2002; 61(4): 399-403.
24. Kokate CK, Gokhale SB, Purohit AP. Pharmacognosy. 32nd ed. Nirali Prakashan: New Delhi; 2005.111-13.
25. Asokan J. Botanical microtechnique principles and practice.1st edn. Plant anatomy research centre: Chennai; 2007.
26. Johansen DA. Plant Microtechnique. MC Graw hill: Newyork; 1940, 523.
27. Evan WC. Trease and Evans Pharmacognosy. 15th edn. Elsevier: London Saunders; 2002. P563-70.
28. WHO. Quality control methods for medicinal plant materials. World Health Organisation: Geneva; 1998.
29. Anonymous. The Ayurvedic Pharmacopoeia of India. Part â€“I, Vol 11, 1st ed. Ministry of Health and family welfare, Department of Indian system of medicine and Homeopathy: New Delhi; 1999, 183- 96.
30. Anonymous. The Indian Pharmacopoeia. Vol 11. Ministry of Health and family welfare: New Delhi; 1996, A- 53, 54, 89.
31. Mukherjee PK. Quality control of Herbal drugs- An approach to evaluation of botanicals 1st edn. Business Horizon: New Delhi; 2002.
32. Wallis TE. Text book of Pharmacognosy. 5th ed. CBS Publishers and Distributors: New Delhi; 1997. 111-17, 561-63.
33. Kokate CK, Purohit AP, Gokhale SB. Practical Pharmacognosy. 4th ed. Vallabh prakashan: New Delhi; 1997. 117-19, 123-25.
34. Patel S, Zaveri M. Pharmacognostic study of the root of Justicia gendarussa Burm. J Trad Med 2011; 6(2): 61-72.
35. Singh S, Machawal L, Chauhan MG. Pharmacognostic study of male leaves of Trichosanthes dioica Roxb. with special emphasis on microscopic technique. J Pharmacognosy Phytother 2010; 2(5): 71-75.
36. Nayak BS, Patel KN. Pharmacognostic studies of the Jatropha curcas leaves. Int J Pharm Tech Res 2010; 2(1): 140-143.
37. Thomas S, Patil DA, Patil AG, Chandra N. Pharmacognostic evaluation and physicochemical analysis of Averrhoa carambola L. fruit. J Herb Toxicol 2008; 2(2): 51-54.
38. Yashvanth S. Rani SS. Rao AS. Madhavendra SS. Anatomical exploration of Leucas aspera (Willd) links a medicinal herb and its pharmacognostic relevance. J Pharma Res 2011; 4(12):4777-79.