VITIS VINIFERA.L (VITACEAE) LEAVES TOWARDS ANTIMITOTIC AND ANTIPROLIFERATIVE ACTIVITY IN ANTICANCER DRUG DISCOVERY


karthikeyan venkatachalam

Abstract


Objective: To prescreen the in vivo antimitotic and antiproliferative activity of the leaves of Vitis vinifera.L. Family Vitaceae using the model organism Yeast (Saccharomyces cerevisiae)

Method: In the present study to investigate the effect of the ethanolic extract of the leaves of the   V. vinifera (Vitaceae), Common Grape Vine was selected for phytochemical and pharmacological screening of antimitotic and antiproliferative activity. Antimitotic activity was evaluated on actively dividing meristamatic cells of Allium cepa root tip and antiproliferative activity was determined by cell viability and DNA fragmentation assay using yeast (Saccharomyces cerevisiae) as a model organism.

Result:  Preliminary phytochemical screening of ethanolic extract of V.vinifera leaves (EEVV) showed the presence of flavonoids, sterols, triterpenoids, saponins, tannins, xanthoprotein, reducing sugars, aromatic acids, phenolic compounds, volatileoil and absence of alkaloids, fixed oils. HPTLC analysis showed the presence of stilbene derivative resveratrol. Antimitoticactivity of EEVV using the Allium cepa root tip model showed good inhibition of dividing meristamatic cells. The percentage of mitotic index (4, 5, 6 mg/ml) of EEVV and control were found to be 29.43, 21.75, 13.96 and 66.48 respectively. The effect was comparable to that of the standard drug methotrexate 100ng/ml (11.69).

The antiproliferative assay using the yeast (Saccharomyces cerevisiae) model also showed that the EEVV is a good inhibitor of yeast cell growth. The percentage of inhibition of cell viability by EEVV (4, 5, 6 mg/ml) is 48.02, 65.56, and 83.05 respectively which was comparable to that of the standard drug methotrexate 50 and 100ng/ml 48.96 and 81.92 respectively. This activity was found to be dose dependent and the IC50 was 4.54mg/ml. DNA fragmentation assay showed that the mode of action of extract is due to the DNA fragmentation.

Conclusion: V. vinifera leaves have been used in medicine due to various biological activities and as a food. This study indicates that the EEVV possesses potential anti mitotic and anti proliferative activity. The presence of resveratrol and the attributed reported anti oxidant activity appears to contribute to the antimitotic and antiproliferative activity. Further investigation requires confirming this activity.


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References


Dixit S, Ali H. Anticancer activity of Medicinal plant extract - A review. J Chem & Cheml Sci 2010; 1: 79-85.

Anonymous. The Wealth of India Raw materials, Sp-W, Vol X, New Delhi, National Institute of Science communication and information resources (NISCAIR), CSIR; 2005. 527-58.

Fernandes F, Ramalhosa E, Pires P, Verdial J, Valentao P, Andrade P, Bento A, Pereira JA. Vitis vinifera Leaves towards bioactivity. J Indu Crop & Pro 2012; 43: 434-40.

Udenigue CC, Ramprasath VR, Aluko RE, Jones PJH. Potential of resveratrol in anticancer and anti-inflammatory therapy. Nutrition Reviews 2008; 66(8):445-54.

Qaddouri B, Guaadaoui A, Bellirou A, Hamal A, Melhaoui A, Brown GW, Bellaoui M. Budding Yeast “Saccharomyces cerevisiae” as a Drug Discovery Tool to identify Plant-Derived Natural Products with Anti-Proliferative Properties. Evid Based complement Alternat Med 2011; 5pages. Article ID954140 doi:10.1093/ecam/nep069.

Kokate CK, Gokhale SB, Purohit AP. Pharmacognosy. 46th Edn. New Delhi. Nirali Prakashan; 2010.p.A.1-6.

Mukherjee PK. Quality control of herbal drugs- An approach to evaluation of botanicals 1st edn New Delhi, Business Horizon; 2012.

Eftehari M, Alizadeh M, Ebrahimi P. Evaluation of the total phenolics and quercetin content of foliage in mycorrhizal grape (Vitis vinifera L.) varieties and effects of postharvest drying on quercetin yield. Industrial Crops and Products 2012; 38: 160-65.

Katalinic V, Generalic I, Skroza D, Ljubenkov I, Teskera A, Konta I, Boban M. Insight in the phenolic composition and antioxidative properties of Vitis vinifera leaves extracts. Croat. J. Food Sci. Tech 2009; 1(2):7-15.

Thenmozhi A, Rao USM. Evaluation of antimitotic activity of Solanum torvum using Allium cepa root meristamatic cells and anticancer activity using MCF-7-Human mammary gland breast Adenocarcinoma cell lines. Drug Invention Today 2011; 3(12):290-96.

Saboo S, Deore SL, Khadabadi SS, Deokate UA. Evaluation of antimitotic and anticancer activity of the crude extracts of Pterospermum acerifolium willd leaves (sterculiaceae). Nig. J. Nat. Prod. & Med 2007; 11:75-79.

Harju S, Fedosyuk H, Peterson KR. Rapid isolation of yeast genomic DNA: Bust n’ Grab. BMC Biotechnology 2004; 4:8-12.

Orhan DD, Orhan N, Ozcelik B, Ergun F. Biological activities of Vitis vinifera L. leaves. Turk J Biol 2009; 33:341-48.

Hawary SE, Fouly KE, Gohary HME, Meselhy KM, Slem A, Talaat Z. Phytochemical and Biological Investigation of Vitis vinifera L. (Flame cultivar), Family Vitaceae Cultivated in Egypt. Nat Sci 2012; 10(10):48-59.

Katalinic V, Mozina SS, Generalic I, Skroza D, Ljubenkov I, Klancnik A. Phenolic Profile, Antioxidant capacity, and Antimicrobial Activity of Leaf Extracts from Six Vitis vinifera L. Varieties. International Journal of Food Properties 2013; 16:45-60.

Singh J, Singh AK, George M, Joseph L. Antiinflammatory and antipyretic activity of Vitis vinifera Leaves extract. T. Ph. Res 2010; 3: 172-82.

Abramovic H, Terpinc P, Generalic I, Skroza D, Klancnik A, Katalinic V, Mozina SS. Antioxidant and antimicrobial activity of extracts obtained from rosemary (Rosmarinus officinalis) and vine (Vitis vinifera) leaves. Croat. J. Food Sci. Technol 2012; 4(1):1-8.

Askari GA, Kahouadji A, Mousaddak M, Ouaffak L, Charof R, Menname Z. Evaluation of Antimicrobial activity of aqueous and ethanolic extracts of Leaves of Vitis vinifera collected from different regions in Morocco. American-Eurasian J. Agric. & Environ. Sci 2012; 12(1):85-90.

Patil PB, Patil SB, Naikwade NS. Evaluation of antitumor and antioxidant activity of Vitis vinifera L. against Ehrlich Ascites Carcinoma induced mice. IJPRD 2011; 3(3):65-72.

Sadaiah B, Kumar KTS, Kavitha CHN, Babu SM, Reddy VP. Antinociceptive activity of methanolic extract of leaves of Vitis vinifera. Der. Pharmacia Sinica 2011; 2(2):190-97.

Kumar CH, Ramesh A, Kumar JNS, Ishaq BM. A Review on hepatoprotective activity of medicinal plants. IJPSR 2011; 2(3): 501-15.

Hakim A. Diversity of secondary metabolites from Genus Artocarpus (Moraceae).Nusantara Bio sci 2010; 2(3):146-56.

Balik J, Kyselakova M, Vrchotova N, Triska J, Kumsta M, Veverka J, Hic P, Totusek J, Lefnerova D. Relations between Polyphenols Content and Antioxidant Activity in Vine Grapes and Leaves. Czech J. Food Sci 2008; 26: S25-S32.

Wang W, Tang K, Yang HR, Wen PF, Zhang P, Wang HL, Huang WD. Distribution of resveratrol and stilbene synthase in young grape plants (Vitis vinifera L. cv. Cabernet Sauvignon) and the effect of UV-C units accumulation. Plant Physiology and Biochemistry 2010; 48: 142-52.




About this article

Title

VITIS VINIFERA.L (VITACEAE) LEAVES TOWARDS ANTIMITOTIC AND ANTIPROLIFERATIVE ACTIVITY IN ANTICANCER DRUG DISCOVERY

Date

01-10-2013

Additional Links

Manuscript Submission

Journal

Innovare Journal of Health Sciences
Vol 1 Issue 3 2013 (October-December) Page: 32-35

Online ISSN

2347-5536

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Authors & Affiliations

karthikeyan venkatachalam
THE TAMILNADU DR.MGR MEDICAL UNIVERSITY, CHENNAI, INDIA.
India


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