TOTAL FLAVONOID CONTENT AND ANTIMICROBIAL PROPERTIES OF FOUR SPECIES OF ZINGIBERACEAE
Objectives: The objectives of this research were to study flavonoid content and antimicrobial activity of four species of Zingiberaceae plants that were collected randomly in West Java (Indonesia).
Methods: Preparation of extract were perfomed by reflux using ethanol as a solvent. The extracts were vaporated using rotavapor. Total flavonoid content was measured using modified method adapted from Chang. For antimicrobial activity microdilution broth method was used.
Results: Antimicrobial activity of four Zingiberaceae plants showed various potency. Hornstedtia pininga, Amomum hochreutineri and Hedychium roxburghii showed strong activity against Bacilus subtilis (MIC at 500 Î¼g/mL). The strongest activity against E. coli were showed by H. pininga and H. roxburghii the MIC were 31.25 Î¼g/mL. Whereas, the strongest antimicrobial activity against P. aeruginosa was H. roxburghii (MIC was 62.5 Î¼g/mL). In line with total flavonoid content results, the highest yield was obtained by H. roxburghii rhizome extract (0.162%), followed by A. hochreutineri (0.76%), Nicolaia hemisphaerica (0.071%) and H. pininga (0.04%).
Conclusion: In the present study, ethanolic extract of four Zingiberaceae showed an antimicrobial activity with various potency. Hornstedtia pininga, Amomum hochreutineri and Hedychium roxburghii showed strong activity against Bacilus subtilis (MIC at 500 Î¼g/mL).
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