METHOD DEVELOPMENT FOR SIMULTANEOUS ANALYSIS OF MARKER SCOPOLETINE, ANDROGRAPHOLIDE, QUERCETIN, AND LUTEOLIN IN ANTIHYPERTENSION JAMU FORMULATION USING RP-HPLC
Keywords:Antihypertension jamu formulation, Scopoletine, Andrographolide, Quercetin, Luteolin, HPLC
Objective: The aim of this research was to develop a method of simultaneous analysis marker compounds in antihypertension jamu formulation using reverse phase high performance liquid chromatography (RP-HPLC).
Methods: Stationary phase, mobile phase, sample preparation and instrument condition were optimized.
Results: Analysis of marker compounds in anti hypertension jamu formulation using HPLC with reverse phase ODS C18 column (LiChroCARTÂ®, 125 x 4.6 mm, particle size of 5 Âµm) and gradient mobile phase of formic acid 1% and methanol could separate markers in 35 min at flow rate 2 mL/min. Four markers in antihypertension jamu formulation have been successfully analyzed simultaneously, were scopoletine, andrographolide, quercetin, and luteolin. The RSD values demonstrated the intra and inter-day precisions were less than 4% and 6.5% respectively and the recoveries around 93.45-114.42%, respectively. All calibration curves showed good linear correlation coefficient which of R > 0.995.
Conclusion: RP-HPLC method has been developed for simultaneous analysis of four markers in antihypertension jamu. The methods can be used to analyze antihypertension jamu formulation in the market.
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