• Irda Fidrianny School of Pharmacy, Bandung Institute of Technology, Indonesia.
  • Kiki Rizki R. School of Pharmacy, Bandung Institute of Technology, Indonesia.
  • M. Insanu School of Pharmacy, Bandung Institute of Technology, Indonesia.


Objectives: The objectives of this research were to study antioxidant capacity from various extracts of banana peels using two methods of antioxidant testing which were ABTS (2.2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) and DPPH (2.2-diphenyl-1-picrylhydrazyl) and correlation of total phenolic, flavonoid and carotenoid content in various extracts of banana peels with ABTS and DPPH antioxidant capacities.

Methods: Extraction was conducted by reflux using various solvents. The extracts were vaporated using rotavapor. Then antioxidant capacities were tested using ABTS and DPPH assays. Determination of total phenolic, flavonoid and carotenoid content were performed by spectrophotometry UV-Vis and its correlation with ABTS and DPPH antioxidant capacities were analyzed by Pearson method.

Results: AL2 (ethyl acetate peels extract of ambon lumut banana) had the highest ABTS scavenging capacity with IC50 1.91 ppm and MU3 had the highest DPPH scavenging activities with EC50 4.39 ppm. MU2 (ethyl acetate peels extract of muli banana) contained the highest total phenolic (3.99 g GAE/100 g), MU2 had highest flavonoid content (6.08 g QE/100 g) and MU2 had also the highest carotenoid 0.34 g BET/100 g.

Conclusions. There was positively high correlation between total phenolic content in muli banana peels with its antioxidant activity using DPPH assays. ABTS scavenging capacities in muli banana and ambon lumut banana peels had positively high correlation with their DPPH scavenging activities.

Keywords: Antioxidants, ABTS, DPPH, Banana peels, Flavonoid, Phenolic, Carotenoid


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1. Jain N, Goyal S, Ramawat KG Evaluation of antioxidant properties and total phenolic content of medicinal plants used in diet therapy during postpartum healthcare in Rajasthan. Int J Pharm Pharm Sci 2011;3(3):248-53.
2. Mallick C, Chatterjee K, Biswas MG, Ghosh D Antihyperglycemic effects of separate and composite extract of root of Musa paradisiaca and leaf of Coccinia indica in Streptozotocin-induced diabetic male albino rat. Afr J Tradit Complement Altern Med 2007;4(3):362–71.
3. Thaipong K, Boonprakob U, Crosby K, Zevallos LC, Byrne DH Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. J Food Comp Anal 2006;19:669-75.
4. Fawole OA, Makunga NP, Opara UL Antibacterial, antioxidant and tyrosinase-inhibition activities of pomegranate fruit peel methanolic extract. J BMC Complement Altern Med 2012;12:200-18.
5. Ling, LT and Palanisamy, UD Review:Potential antioxidants from tropical plants, In:Valdez, B., editor, Food industrial processes-methods, Kuala Lumpur:In Tech;1999. p. 64-72.
6. Souri E, Amin G, Farsan H, Barazandeh TM Screening antioxidant activity and phenolic content of 24 medicinal plats extracts. DARU J Pharm Sci 2008;16:83-7.
7. Luis A, Gil N, Amaral ME, Duarte AP Antioxidant activities of extract from Acacia melanoxylon, Acacia dealbata, Olea europaea and alkaloids estimation. Int J Pharm Pharm Sci 2012;4:225-31.
8. Oboh G, Ademosun AO Characterization of the antioxidant properties of phenolic extracts from some citrus peels. J Food Sci Technol 2012;49(6):729-36.
9. Huang WY, Zhang HC, Liu WX, Li CY. Survey of antioxidant capacity and phenolic composition of blueberry, blackberry, and strawberry in Nanjing. J Zhejiang Univ Sci B 2012;13(2):94-102.
10. Agbor GA, Moumbegna P, Oluwasola EO, Nwosu LU, Njoku RCC, Kanu S, Emekabasi EI, et al. Antioxidant capacity of some plant foods and beverages consumed in the eastern region of Nigeria. Afr J Tradit Complement Altern Med 2011;8(4):362-9.
11. Blois MS Antioxidant determination by the use of stable free radicals. J Nature 1958;181:1199-2000.
12. Bedawey AA Characteristics of antioxidant isolated from some plants sources, Cairo:Shibin El-Kom;2010. p. 1-11.
13. Li XC, Wang XZ, Chen DF, Chen SZ. Antioxidant activity and mechanism of protochatechuic acid in vitro. J Funct Food Health Dis 2011;1:232-44.
14. Pellegrini N, Serafini M, Colombi B, Rio DD, Salvatore S Total antioxidant capacity of plant food, beverages and oils consumed in Italy assessed by three different in vitro assays. J Am Coll Nutr 2003;133:2812-19.
15. Chang CC, Yang MH, Wen HM, Chern JC. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal 2002;10:178-82.
16. Pourmorad F, Hosseinimehr SJ, Shahabimajd N. Antioxidant activity, phenol and flavonoid content of some selected Iranian medicinal plants. Afr J Biotechnol 2006;5(11):1142-5.
17. Apak R, Guclu K, Demirata B, Ozyurek M, Celik SE, Bektasoglu B, Berker KI, Ozyurt D. Comparative evaluation of various total antioxidant capacity assays applied to phenolic compounds with the CUPRAC assay. J Molecules 2007;12:1496-1547.
18. Karuppiah P, Mustaffa M. Antibacterial and antioxidant activities of Musa sp. leaf extracts against multidrug resistant clinical pathogens causing nosocomial infection. Asian Pac J Trop Biomed 2013;3(9):737-42.
19. Shodehinde SA, Oboh G. Antioxidant properties of aqueous extracts of unripe Musa paradisiaca on sodium nitroprusside induced lipid peroxidation in rat pancreas in vitro. Asian Pac J Trop Biomed 2013;3(6):449-57.
20. Heim KE, Tagliaferro AR, Bobilya DJ. Flavonoid antioxidants:Chemistry, metabolism and structure-activity relationships. J Nutr Biochem 2002;13:572-84.
21. Foote, CS Free radicals in biology. 3rd ed. New York:Academic Press;1976.
22. Beutner S, Bloedorn B, Hoffmann T, Martin HD Synthetic singlet oxygen quenchers. J Methods Enzymol 2000;319:226-41.
23. Kobayashi M, Sakamoto Y Singlet oxygen quenching ability of astaxanthin esters from the green alga Haematococcus pluvialis. J Biotech Lett 1999;21:265-9.
24. Müller L, Fröhlich K, Böhm V. Comparative antioxidant activities of carotenoids measured by ferric reducing antioxidant power (FRAP), ABTS bleaching assay (aTEAC), DPPH assay and peroxyl radical scavenging assay. J Food Chem 2011;129:139-48.
25. Charles DJ. Antioxidant properties of spices herbs and other. London:John Willey;2013.p.
26. Huang D, Ou B, Prior RL The chemistry behind antioxidant capacity assays. J Agric Food Chem 2005;53:1841-56.
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How to Cite
Fidrianny, I., K. R. R., and M. Insanu. “IN VITRO ANTIOXIDANT ACTIVITIES FROM VARIOUS EXTRACTS OF BANANA PEELS USING ABTS, DPPH ASSAYS AND CORRELATION WITH PHENOLIC, FLAVONOID, CAROTENOID CONTENT”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 6, no. 8, Aug. 2014, pp. 299-03,
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