VALIDATED SPECTROPHOTOMETRIC METHOD TO DETERMINE VARDENAFIL AND SILDENAFIL IN PHARMACEUTICAL FORMS USING POTASSIUM IODIDE AND POTASSIUM IODATE


Amir Alhaj Sakur, Shaza Affas

Abstract


Objective: To develop and validate simple, sensitive, precise and free of organic solvents method for the determination of sildenafil (SIL) and vardenafil (VAR) in bulk and pharmaceutical formulation.

Methods: The method is based on the reaction of studied drugs with a mixture of potassium iodide and potassium iodate in an aqueous medium at (25±2 °C) to form yellow coloured triiodide ions (I3-) within 45 min. The reaction is followed spectrophotometrically by measuring the absorbance at 288, 351 nm and 285, 351 nm for sildenafil and vardenafil respectively.

Results: The effects of analytical parameters on the reported systems were investigated. Beer's law of SIL was obeyed in the range of (0.4-12) μg ml-1and (0.6-16) μg ml-1. Molar absorptivity was found to be (67.659 ×103) lmol/cmand (37.955×103) lmol/cm at 288 nm, 351 nm respectively. Beer's law of VAR was obeyed in the range of (0.2-13) μg/mland (0.5-40) μg/ml. Moreover, molar absorptivity’s were found to be (68.719 ×103) l mol-1 cm-1and (26.691×103) l mol-1 cm-1 at 285 nm, 351 nm respectively.

Conclusion: The proposed method has been applied to determine the components in dosage forms with an average recovery of 98.15% to 103.45% and the results have been found in good agreement with those results obtained by the reference methods.


Keywords


Sildenafil citrate, Vardenafil hydrochloride, Spectrophotometry, Potassium iodide, Potassium Iodate, Triiodide ion

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References


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About this article

Title

VALIDATED SPECTROPHOTOMETRIC METHOD TO DETERMINE VARDENAFIL AND SILDENAFIL IN PHARMACEUTICAL FORMS USING POTASSIUM IODIDE AND POTASSIUM IODATE

Keywords

Sildenafil citrate, Vardenafil hydrochloride, Spectrophotometry, Potassium iodide, Potassium Iodate, Triiodide ion

DOI

10.22159/ijpps.2017v9i11.20578

Date

01-11-2017

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 9, Issue 11, 2017 Page: 65-69

Online ISSN

0975-1491

Statistics

61 Views | 6 Downloads

Authors & Affiliations

Amir Alhaj Sakur
Analytical and Food Chemistry Department, Faculty of Pharmacy, University of Aleppo, Syria
Syrian Arab Republic

Shaza Affas
Analytical and Food Chemistry Department, Faculty of Pharmacy, University of Aleppo, Syria.


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