NEW FLUORESCENCE QUENCHING BASED METHOD FOR THE DETERMINATION OF TRANDOLAPRIL IN BULK AND CAPSULES
Keywords:Fluorescence quenching, Fluorescein, Trandolapril, Charge transfer complex
Objective: The objective of the method was to develop a new, simple, rapid and accurate spectrofluorimetric method for the determination of trandolapril in bulk and capsules.
Methods: Trandolapril reacts with fluorescein to form a charge transfer complex which results in fluorescence quenching of the fluorescein dye. The fluorescence quenching intensity was measured at 515 nm after excitation at 470 nm.
Results: Under the optimum conditions, the quenched fluorescence intensity was linear with the concentration of trandolapril in the range of 1.80 â€“ 9.60 mg/mL (4.18Â´10âˆ’6 â€“ 22.30Â´10âˆ’6 M) (R2 = 0.9983) with a detection limit of 0.345 mg/mL. In order to validate the method the results were compared with those obtained by a high performance liquid chromatography method. The proposed method was successfully applied to the analysis of trandolapril in pure form and capsules with good precision and accuracy compared to the reported method as revealed by t- and F- tests.
Conclusion: The developed method was simple, fast, accurate and precise. It could be applied for routine quality control analysis of trandolapril in its pure form and in capsules.
British National Formulary (BNF). 67th ed. British Medical Association and Royal Pharmaceutical Society of Great Britain; 2014.
Rambabu C, Ramu G, Biksham AB, Venkata SR. Optimized and validated reverse phase HPLC method for the determination of trandolapril in bulk and formulations. Rasayan J Chem 2010;3(2):777-82.
Gumustas M, Sanli S, Sanli N, Ozkan SA. Development and validation of a liquid chromatographic method for concurrent assay of weakly basic drug verapamil and amphoteric drug trandolapril in pharmaceutical formulations. J Food Drug Anal 2012;20(3):588-96.
Sahu K, Karthikeyan C, Moorthy NSH, Trivedi P. A Validated UPLC method used for the determination of trandolapril and its degradation products as per ICH guidelines. Curr Pharm Anal 2011;7:182-8.
Gumieniczek A, HopkaÅ‚a H. High-performance liquid chromatographic assay of trandolapril in capsules. Acta Pol Pharm 2000;57(4):253-5.
Sankar ASK, Alekhya M, Sythana S, Ravichandiran V. Analytical method development and validation of trandolapril in tablets by RP-HPLC. Int J Front Sci Technol 2013;1(2):119.
Deore MG, Laddha SS. Development and validation of a stability indicating HPLC assay method for simultaneous determination of trandolapril and verapamil HCl in tablet dosage form. Int J Pharm Res Dev 2014;6(3):138-47.
Dubey SK, Deshpande S, Kumar S, Raut P, Jain AK, Mudakavi RJ. A high performance liquid chromatographic method for quantification of trandolapril using UV spectrometric detection. Int J Biomed Res 2010;1(3):133-40.
Gumieniczeka A, Hopkalaa H. Development and validation of a liquid chromatographic method for the determination of trandolapril and verapamil in capsules. J Liq Chromatogr Relat Technol 2001;24(3):393-400.
Pistos C, Koutsopoulou M, Panderi I. Liquid chromatographic tandem mass spectrometric determination of trandolapril in human plasma. Anal Chim Acta 2005;540(2):375-82.
Sreekanth N, Awen BZ, Rao CB. HPTLC method development and validation of trandolapril in bulk and pharmaceutical dosage forms. J Adv Pharm Technol Res 2010;1(2):172-9.
Gandhi SP, Dewani MG, Borole TC, Damle MC. Development and validation of stability indicating HPTLC method for determination of trandolapril as bulk drug. Asian J Res Chem 2011;4(3):437.
Subbayamma AV, Rambabu C. Spectrophotometric determination of trandolapril and aripiprazole in pharmaceutical dosage forms with citric acid-acetic anhydride reagent. Oriental J Chem 2010;26(1):151-4.
New Drugs. Analysis of intrauterine malformations of the vertebral column induced by oxygen deficiency. Can Med Assoc J 1959;80(12):997-98.
Xia H, Gong G, Zhao H, Li HL. Fluorometric determination of arsenic (III) with fluorescein. Microchem J 1997;56:327-31.
Lakowicz JR. Principles of fluorescence spectroscopy. 3rd ed. Springer; 2006.
International conference on harmonization of technical requirements for registration of pharmaceuticals for human use. Validation of analytical procedures: Text and methodology ICH Q2 (R1); 2005.
Harvey D. Modern analytical chemistry. Second ed. David Harvey; 2009.