• Hanan Shasho Quality Control and Pharmaceutical Chemistry Department, University of Aleppo-Syrian Arab Republic
  • Amir Alhaj Sakur Analytical Chemistry Department-University of Aleppo-Syrian Arab Republic
  • Saleh Trefi Quality Control and Pharmaceutical Chemistry Department, University of Aleppo-Syrian Arab Republic


Objective: The objective of this study was to develop and validate a single HPLC method, in order to separate and assay four antihistamine drugs diphenhydramine, chlorpheniramine, cyproheptadine and fexofenadine in pharmaceutical forms. This method was a practical additional choice in quality control laboratories.

Methods: The chromatographic conditions comprised of a classical C18-type stationary phase (150 × 4.6 mm, 5μ), with a mobile phase consisting of, 2.5g of sodium octane sulfonic acid in a mixture of 500 ml of deionized water and 500 ml of acetonitrile, and apparent pH of 2.0 was adjusted with phosphoric acid. The flow rate was 1 ml/min; the detection wavelengths were at 220 nm, 230 nm, 265 nm and 254 nm for diphenhydramine, chlorpheniramine, cyproheptadine and fexofenadine respectively. The temperature was ambient temperature.

Results: The method was validated for linearity with correlation coefficients very close to one, the accuracy with mean recovery values between 95.0-105.0%, precision with relative standard deviations of the calculated concentrations less than 5.0% and specificity in the presence of degradation products. Then it was used successfully to separate a mixture of them and to assay these drugs in pharmaceutical forms purchased from Syria.

Conclusion: The results presented in this paper showed that the developed method was simple and applicable, for the separation and determination of the four drugs in their pharmaceutical forms.

Keywords: HPLC, Diphenhydramine, Chlorpheniramine, Cyproheptadine, Fexofenadine


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How to Cite
Shasho, H., A. A. Sakur, and S. Trefi. “SEPARATION AND ASSAY OF FOUR ANTIHISTAMINE DRUGS DIPHENHYDRAMINE, CHLORPHENIRAMINE, CYPROHEPTADINE AND FEXOFENADINE IN PHARMACEUTICAL FORMS BY A SINGLE HPLC METHOD”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 10, no. 4, Apr. 2018, pp. 53-60, doi:10.22159/ijpps.2018v10i4.24819.
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