NEPHRO-PROTECTIVE ACTIVITY OF ISOLATED METHANOL FRACTIONS PHYTO-COMPOUND FROM BARK OF TERMINALIA ARJUNA


Shreya Mandal, Arpita Patra, Shrabani Pradhan, Suchismita Roy, Animesh Samanta, Koushik Das, Atiskumar Chattopadhyay, Dilip Kumar Nandi

Abstract


Objective: The aim of this study was to evaluate the antioxidant property of the isolated phytocompounds from TA (Terminalia arjuna) bark and in vivo study for nephro-protective and oxidative stress reducing activity in experimentally induced albino male rats.

Methods: Fractions from methanol crude TA extract were collected by column chromatography and F27, F28, F29 fractions were selected on the basis of antioxidant property by 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay. The in vivo study performed by 30 albino male rats which were randomly divided into five groups: Group I (control)were taken normal food and water, Groups II (uremic) were injected acetaminophen intraperitoneally at the dose of 500 mg/kg/d for 10 d, Group III, IV and V(extract treatment) acetaminophen intraperitoneally at the dose of 500 mg/kg/d for 10 d with co-administered orally of methanol fraction F27, F28, F29 at the dose of 100 mg/kg/d for 15 d respectively.

Results: After scarification of rats, the uremic marker plasma urea (80%), creatinine (85%) were elevated and antioxidant enzyme marker such as plasma SOD and catalase level were significantly increased (p<0.05)in Group IV compared to Group II. The total phenolic content of the F28 methanolic fraction was (815.48±8.11) mg gallic acid equivalent/g of extract. For isolation of available compound by 1H NMR study in F28 methanol fraction of TA bark was arjunoside IV which contained olefinic proton (a pair of carbon atom linked with double bond).

Conclusion: Among the three methanolic fraction of TA bark, F28 was shown best antioxidative, nephron-protective and oxidative stress reducing property. 


Keywords


Terminalia arjuna, Column chromatography, DPPH, 1H NMR, Kidney disease

| PDF | HTML |

References


Jawala S, Gupta AK, Singla R, Gupta V. General awareness and relative popularity of Allopathic, Ayurvedic and homeopathic system. J Chem Pharm Res 2009;1:105-12.

Pradhan S, Mandal S, Roy S, Mandal A, Das K, Nandi DK. Attenuation of uremia by orally feeding alpha-lipoic acid on acetaminophen induced uremic rats. Saudi Pharm J 2013;21:187–92.

Mandal A, Nandi DK, Roy S, Das K, Mondal KC. In vivo assessment of bacterio therapy on acetaminophen induced uremic rats. J Nephrol 2013;26:228-36.

Sreenath KB, Sundaram S, Gopalakrishnan VK, Poornima K. Quantitative phytochemical analysis, in vitro antioxidant potential and gas chromatography-mass spectrometry studies in ethanolic extract of Azolla microphylla. Asian J Pharm Clin Res 2016;9:318-23.

Vasu K, Goud JV, Suryam A, Singara Chary MA. Biomolecular and phytochemical analyses of three aquatic angiosperms. Afr J Microbiol Res 2009;3:418-21.

Kinsalin VA, Kumar PS, Duraipandiyan V, Ignacimuthu S, Al-Dhabi NA. Antimicrobial activity of methanol extracts of some traditional medicinal plants from Tamil Nadu, India. Asian J Pharm Clin Res 2014;7 Suppl 1:36-40.

Patra JK, Gouda S, Sahoo SK, Thatoi HN. Chromatography separation. 1H NMR analysis and bioautography screening of methanol extract of Excoecaria agallocha L. from Bhitarkanika, Orissa, India. Asian Pac J Trop Biomed 2012;2:50-6.

Barros L, Falcao S, Baptista P, Freire C, Vilas-Boas M, Ferreira IC. Antioxidant activity of Agaricus sp. mushrooms by chemical, biochemical and electrochemical assays. Food Chem 2008; 111:61-6.

Olfert ED, Cross BM, McWilliam AA. Guide to the care and use of experimental animals. 2nd ed. Canada: Ottawa; 1993.

Burtis CA, Ashwood ER. Tietz Textbook of Clinical Chemistry. 3rd ed. Philadelphia: 1999.

Singh RK, Gautam RK, Karchuli MS. Evaluation of nephroprotective activity of Mentha arvensis in cisplatin-induced nephrotoxicity. Asian J Pharm Clin Res 2014;7:188-91.

Praharsheta Am, Samuel V, Nirmala P. Role of ebselen, a selenoorgano compound in cisplatin induced nephrotoxicity in wistar rats. Int J Curr Pharm Res 2015;7:57-9.

Marklund S, Marklund G. Involvement of superoxide anion radical in auto-oxidation of pyrogallol and a convenient assay of superoxide dismutase. Eur J Biochem 1974;47:469–74.

Demiray S, Pintado ME, Castro PML. Evaluation of phenolic profiles and antioxidant activities of turkish medicinal plants: Tilia argentea, Crataegi folium leaves and Polygonum bistorta roots. World Acad Sci Eng Technol 2009;54:312-17.

Haque AM, Hassan MM, Das A, Begum B, Ali MY, Morshed H. Phytochemical investigation of Vernonia cinerea. J Appl Pharm Sci 2012;2:79-83.

Arts CW, Hollman PCH. Polyphenols and disease risk in epidemiologic studies. Am J Clin Nutr 2005;81:317–25.

Chang-Claude J, Frentzel-Beyne R, Eilber U. Mortality pattern of German vegetarians after 11 y of follow up. Epidemiology 1992;3:395–401.

Graf BA, Milbury PE, Blumberg JB. Flavonols, flavonones, flavanones and human health: Epidemiological evidence. J Med Food 2005;8:281–90.

Phillips R, Lemon F, Beeson L, Kuzma J. Coronary heart disease mortality among Seventh-Day Adventists with differing dietary habits: a preliminary report. Am J Clin Nutr 1978;31:191–8.

Nema R, Jain P, Khare S, Pradhan A, Gupta A, Singh D. Antibacterial and antifungal activity of Terminalia arjuna leaves extract with special reference to flavonoids. Basic Res J Med Clin Sci 2012;1:63-5.

Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxidation in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95:351–8.

Mayne PD. The kidneys and renal calculi. In: Clinical chemistry in diagnosis and treatment (Eds.). London: Edward Arnold Publications; 1994.

Anwar S, Khan NA, Amin KMY, Ahmad G. Effects of Banadiq-al Buzoor in Some Renal Disorders. Pakistan: Karachi; 1999.

Bennit WM, Parker RA, Elliot WC, Gilbert DN, Houghton DC. Sex related differences in the susceptibility of rat to gentamicin nephrotoxicity. J Infect Dis 1982;145:370–3.

EI-Sokkary GH, Kamel ES, Reiter RJ. Prophylactic effect melatonin in reducing lead-induced neurotoxicity in the rat. Cell Mol Biol Lett 2003;8:461–70.

Pradhan S, Roy S, Mandal S, Das K, Das S, Nandi DK. Protective effect of alpha-lipoic acid on hepato renal toxicity on acetaminophen induced uremic male albino rat. Eur J Biomed Pharm Sci 2016;3:668-74.

Debnath D, Mandal TK. Study of quinalphos (an environmental oestrogenic insecticide) formulation (Ekalux 25 E. C.)-induced damage of the testicular tissues and antioxidant defense systems in sprague-dawley albino rats. J Appl Toxicol 2000;20:197-204.

Vaziri ND. Oxidative stress in uremia: nature, mechanisms and potential consequences. Semin Nephrol 2004;24:469–73.




About this article

Title

NEPHRO-PROTECTIVE ACTIVITY OF ISOLATED METHANOL FRACTIONS PHYTO-COMPOUND FROM BARK OF TERMINALIA ARJUNA

Keywords

Terminalia arjuna, Column chromatography, DPPH, 1H NMR, Kidney disease

DOI

10.22159/ijpps.2017v9i12.21274

Date

01-12-2017

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 9, Issue 12, 2017 Page: 175-180

Online ISSN

0975-1491

Statistics

21 Views | 7 Downloads

Authors & Affiliations

Shreya Mandal
Department of Nutrition, Microbiology and Human Physiology, Raja N. L. Khan Women’s College, Paschim Medinipur, Pin-721102, West Bengal, India
India

Arpita Patra
Department of Nutrition, Microbiology and Human Physiology, Raja N. L. Khan Women’s College, Paschim Medinipur, Pin-721102, West Bengal, India

Shrabani Pradhan
Department of Nutrition, Microbiology and Human Physiology, Raja N. L. Khan Women’s College, Paschim Medinipur, Pin-721102, West Bengal, India

Suchismita Roy
Department of Nutrition, Microbiology and Human Physiology, Raja N. L. Khan Women’s College, Paschim Medinipur, Pin-721102, West Bengal, India

Animesh Samanta
Department of Nutrition, Microbiology and Human Physiology, Raja N. L. Khan Women’s College, Paschim Medinipur, Pin-721102, West Bengal, India

Koushik Das
Department of Nutrition, Microbiology and Human Physiology, Raja N. L. Khan Women’s College, Paschim Medinipur, Pin-721102, West Bengal, India

Atiskumar Chattopadhyay
Secretary, Faculty Council of Science, Jadavpur University, Kolkata-700032, West Bengal, India

Dilip Kumar Nandi
Department of Nutrition, Microbiology and Human Physiology, Raja N. L. Khan Women’s College, Paschim Medinipur, Pin-721102, West Bengal, India
India


Article Tools



Refbacks

  • There are currently no refbacks.