ANALYSIS OF FATTY ACIDS FROM OIL OF GREEN TEA (CAMELLIA SINENSIS L) BY GAS CHROMATOGRAPHY COUPLED WITH FLAME IONIZATION DETECTOR AND ITS ANTICANCER AND ANTIBACTERIAL ACTIVITY IN VITRO

  • Koushik Das Raja Narendralal khan Women's College, Vidyasagar University, Paschim Medinipur http://orcid.org/0000-0002-6349-8251
  • Meghamala Mandal Raja Narendralal khan Women's College, Vidyasagar University, Paschim Medinipur
  • Dilip Kumar Nandi Raja Narendralal khan Women's College, Vidyasagar University, Paschim Medinipur
  • Balaram Das Ramnagar College, Purba Medinipur, Pin- 721441, West Bengal, India.
  • Sreenivas R J Food Chemistry Division National Institute of Nutrition (ICMR) Ministry of Health & Family Welfare Hyderabad- 500007

Abstract

Objective: Tea is a widely consumed beverage worldwide. The effect of green tea is mainly due to its high polyphenols-(-) epigallocatechin-3-gallate (EGCG) content in the culture of cancer cell and bacterial cells. The present work was carried out to investigate the efficacy of green tea oil (GTO) against cancer cells and bacterial cells.


Methods: In this study green tea oil was prepared from green tea for different experiment and determination of fatty acids profile from green tea oil. In the present study, peripheral blood lymphocyte (PBL) was chosen as human peripheral blood lymphocytes and blood cancer MCF-7 cells were chosen as human cancer cells. To fulfill our aims and also to evaluate the activity of this phytomedicine against normal lymphocytes and cancer cells the cell samples were divided into 26 experimental groups in the following ways. Each Petri dish contains 2 X 105 cells.


Results: GTO shows a potent anticancer agent but nontoxic to normal cells. The GTO decreases the reduced glutathione (GSH) level and increase the oxidized glutathione (GSSG) level significantly (P<0.05) in MCF-7 cells. But in lymphocytes the GSH level and GSSG level were almost the same with the control group but doxorubicin (DOX) significantly decreased the GSH and increase the GSSG level. Green tea oil treatment causes generation of reactive oxygen species (ROS) in MCF-7 cells revealed by DCFH2DA staining. Agar diffusion test shows the GTO is effective against multi-drug resistant bacteria.


Conclusion: This phytomedicine has a potent anticancer activity without damaging the normal lymphocytes. So, this drug can be used for further treatment of anticancer and antibacterial.

Keywords: Green tea, GTO, Polyphenols- (-) Epigallocatechin-3- gallate, Cancer, Phytomedicine

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Author Biographies

Koushik Das, Raja Narendralal khan Women's College, Vidyasagar University, Paschim Medinipur
In-charge,Department of nutrition, Raja Narendralal Khan Women's College, Mednapore, Paschim Medinipur, West Bengal, Pin- 721102.
Meghamala Mandal, Raja Narendralal khan Women's College, Vidyasagar University, Paschim Medinipur
Research Scholar, Department of Nutrition, Raja Narendralal Khan Women's College, Midnapore, Paschim Medinipur
Dilip Kumar Nandi, Raja Narendralal khan Women's College, Vidyasagar University, Paschim Medinipur
HOD and Associate Professor, Department of Physiology, Raja Narendralal Khan Women,s Colege
Balaram Das, Ramnagar College, Purba Medinipur, Pin- 721441, West Bengal, India.
Teacher, Department of Physiology, Ramnagar College, Purba Medinipur, Pin- 721441, West Bengal, India.
Sreenivas R J, Food Chemistry Division National Institute of Nutrition (ICMR) Ministry of Health & Family Welfare Hyderabad- 500007

Scientist-D/Asst. Director

Food Chemistry Division

National Institute of Nutrition (ICMR)

Ministry of Health & Family Welfare

Hyderabad- 500007

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Das, K., M. Mandal, D. K. Nandi, B. Das, and S. R. J. “ANALYSIS OF FATTY ACIDS FROM OIL OF GREEN TEA (CAMELLIA SINENSIS L) BY GAS CHROMATOGRAPHY COUPLED WITH FLAME IONIZATION DETECTOR AND ITS ANTICANCER AND ANTIBACTERIAL ACTIVITY IN VITRO”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 11, no. 3, Feb. 2019, pp. 104-11, doi:10.22159/ijpps.2019v11i3.29888.
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