PHYTOCHEMICAL SCREENING AND ANTIMICROBIAL ACTIVITY OF THUJA OCCIDENTALIS SEEDS EXTRACTS AGAINST THE ISOLATED COMMON SKIN INFECTING MICROORGANISMS
Objective: This study represents the first attempt to investigate the antimicrobial activity of Thuja occidentalis seeds extracts against the isolated skin infecting microorganisms.
Methods: T. occidentalis seeds were powdered and extracted continuously by Soxhlet apparatus using 96% ethanol to obtain the total crude ethanolic extract. The extracts with increasing polarity were successively prepared with petroleum ether, chloroform, ethyl acetate, and methanol using the Soxhlet apparatus.
The extracts were screened for their phytochemical constituents. Their antimicrobial activity against standards microorganism and the isolated skin infecting microorganisms was evaluated using the agar diffusion method.
Results: The result revealed the presence of flavonoids, alkaloids, terpenoids, tannins and cardiac glycosides. The antimicrobial activity result showed that Escherichia coli, Pseudomonas aeroginosa, Staphylococcus aureus, Bacillus subtilis and Candida albicans were found to be sensitive to the extracts of T. occidentalis seeds with inhibition zones range between 20Â±0.57-12Â±1.25 mm. The isolated skin infecting microorganisms; Staphylococcus aureus and Staphylococcus epidermidis were found to be highly susceptible to the extracts of T. occidentalis seeds with inhibition zones of 28Â±1.02-22Â±0.45 mm and minimum inhibitory concentration (MIC) values of 6.25 mg/ml.Conclusion: From the results it concludes, T. occidentalisseeds extracts had a considerable level of antimicrobial activity. They are more effective towards gram-positive than gram-negative bacteria. Hence, the active principles present in the extracts are particularly powerful effective against the skin infected isolated bacteria. In conclusion, there is a proportional increasing in the antimicrobial activity with increase of the solvents polarity. This may attribute to the fact that the chemical constituents responsible for the antimicrobial activity are of high polarity, such as tannins.
2. Benahmed-Bouhafsoun A, Djied S, Mouzaz F, Kaid-Harche M. Phytochemical composition and in vitro antioxidant activity of Chamaerops humilis L. extracts. Int J Pharm Pharm Sci 2013;5:741-4.
3. Naser B, Bodinet C, Martin Tegtmeier M, Lindequist U. Thuja occidentalis (Arborvitae): a review of its pharmaceutical, pharmacological and clinical properties. J Evidence-Based Complementary Altern Med 2005;2:69-78.
4. Tardif J, Stevenson D. Radial growth-climate association of Thuja occidentalis L. at the northwestern limit of its distribution, Manitoba, Canada. Dendrochronologia 2001;19:2-10.
5. Biswas R, Mandal SK, Dutta S, Bhattacharyya SS, Boujedaini N, Khuda-Bukhsh AR. The fraction of Thuja occidentalis demonstrates major anti-cancer potentials: evidence from in virto studied on A375 Cells. J Evidence-Based Complementary Altern Med 2011; 1:23-9.
6. Felter H. A treatise on Thuja occidentalis. Drug Treatise, Number I. Cincinnati, Ohio: Lloyd Bros; 1904.
7. Pak J, Bot JN, Ahmad M, Mehjabeen M, Ul-haq Z, Alam SM, et al. Antimicrobial screening of some medicinal plants of Pakistan. Pak J Sci Ind Res 2010;43:1773-5.
8. Offergeld R, Reinecker C, Gunz E. Mitogenic activity of high molecular polysaccharide fractions isolate the Cupressaceae Thuja occidentalis L. enhanced cytokine-production the polysaccharide, g fraction (TPSg). Leuk J 1992;6:189-91.
9. McCaig LF, McDonald LC, Mandal S, Jernigan DB. Staphylococcus aureus-associated skin and soft tissue infections in ambulatory care. Emerging Infectious Diseases J 2012;12:443-58.
10. Adeleye IA, Ogunniyi AA, Omonigbehin EA. Antimicrobial activity of some local herbs on common skin pathogens. Biol Res Com 2003;15:231-6.
11. Otto M. Staphyloccus epidermidis-the accidental pathogen. Nat Rev Microbiol 2010;7:555-67.
12. Sofowora A. Recent trends in research into African medicinal plants. J Ethnopharmacol 1993;38:197-208.
13. Evans, William Charles. Trease and Evans' Pharmacognosy. Elsevier Health Sciences; 2009.
14. Kavanagh FS. Analytical microbiology. 2nd edition. Academic Press: New York and London; 1972. p. 11.
15. Cowan M. Plant products as antimicrobial agents. Clin Microbiol Rev 1999;12:564-82.
16. Jawetz E, Janet S, Nicholas L, Edwards E. Skin microorganism. Medical Microbiology, Lange International NY; 1978. p. 25-7.
17. Mann A, Yahaya Y, Banso A, Ajayi GO. Phytochemical and antibacterial screening of Anogeissus leiocarpus against some microorganisms associated with infectious wounds. Afr J Microbiol Res 2008;2:60-2.
18. Jasuja N, Sharma S, Saxena R, Choudhary J, Sharma R, Joshi S. Antibacterial, antioxidant and phytochemical investigation of Thuja occidentalis leaves. J Med Plant Res 2013;7:1886-93.
19. Ho KY, Tsai CC, Huang JS, CP Lin TC, Lin CC. Antimicrobial activity of tannin components from Vaccinium vitis-idaea L. J Pharm Pharmacol 2001;53:187-91.
20. Meigy NM, Meta M, Natsir D. Test of antimicrobial activity of tannins extract from guava leaves to pathogens microbial. Int Asian Res J 2014;2:43-50.
21. Ngoupayo J, Assonfack FRM, Chelea M, Djiele NP, Ndelo J. Evaluation of the antimicrobial activity of tannin extracted from the barks of Erythrophleum guineensis (Caesalpiniaceae). J Pharmacol Phytoch 2016;5:287-91.
22. Lim SH, Darah, Jain K. Antimicrobial activities of tannins extracted from Rhizophora apiculata barks. J Trop Forest Sci 2006;18:59-65.
23. Doss A, Mohammed MH, Dhanabalan R. Antibacterial activity of tannins from the leaves of Solanum trilobatum L. Ind J Sci-Tech 2009;2:41-3.
24. Scalbret A. Antimicrobial properties of tannins. Phytochemistry 1991;30:3875-83.
25. Giovana MLF, Ana LFV, Bianca WB, Silvana G, Saulo FA, Suzelei de CF, et al. Antimicrobial activity and rates of tannins in Stryphnodendron adstringens Mart. Accessions collected in the Brazilian Cerrado. Am J Pant Sci 2013;4:2193-8.