• Ibrahim Siddig Hamid University of medical sciences and technology (UMST)
  • Ikram Mohamed Eltayeb University of medical sciences and technology (UMST)


Objective: This study represents the first attempt to investigate the antimicrobial activity of Peganum harmala, and Trachyspermum ammi seeds extract against the isolated bacillary dysentery-causing microorganisms.

Methods: T. ammi and P. harmala were extracted by 96% ethanol using Soxhlet apparatus. The extracts were screened for their phytochemical constituents. Their antimicrobial activity against the isolated dysentery-causing microorganisms was evaluated using the agar diffusion method.

Results: The antimicrobial activity result showed that, the two isolated bacteria, Shigella flexneri, and Shigella dysenteriae were found to be sensitive to the extract of T. ammi seed with inhibition zones up to 25 mm, compared to the inhibition zone of 20 mm produced by Gentamycin standard drug, this is mainly due to the presence of the different phytochemical in the extract such as tannin, flavonoids, terpenoids which are well known for their antimicrobial effects. The two isolated bacteria were found to be insensitive (zero mm) to P. harmala extract, Amoxicillin, and Amoclan (Amoxicillin+clavulanic acid) standard drugs, this is due to the fact that, the phytochemicals constituents of P. harmala possess the antagonistic effect to each other’s. Addition to; these bacteria became resistant to both Amoxicillin and Amoclan.

Conclusion: From the results it concludes, T. ammi seeds extract had a considerable level of antimicrobial activity against bacillary dysentery-causing microorganisms resistant to Amoxicillin and Amoxicillin+clavulanic acid drugs.

Keywords: Trachyspermu, ammi, Peganum, Harmala, Shigella, Flexneri, Dysenetry


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1. Arunkumar K, KR Chandrashekar. Phytochemical evaluation and in vitro antimicrobial and antioxidant studies of leaf and stem bark extracts of Polyalthia fragrans (dalz.) bedd–an endemic species of Western ghats. Int J Pharm Pharm Sci 2017;9:20-4.
2. Benahmed Bouhafsoun A, Djied S, Mouzaz F, Kaid Harche M. Phytochemical composition and in vitro antioxidant activity of Chamaerops humilis L. extracts. Int J Pharm Pharm Sci 2013;5:741-4.
3. Al-khazraji S, Khalil I. Antinociceptive activity of the alcoholic extract of trachyspermum ammi (L) (Ajwain) in rats. Halabja University J 2016;1:423-44.
4. Mahtab Alam Khan. Introduction and importance of medicinal plant and herbs. Published by Zahid; 2016.
5. Singh I, Singh VP. Antifungal properties of aqueous and organic solution extracts of seed plants against Aspergillus flavus and A. niger. Phytomorphology 2000;50:151-7.
6. Bairwa R, Sodha RS, Rajawat BS. Trachyspermum ammi reviews. Pharmacognosy 2012;6:56.
7. Choudhury S, Ahmed R, Kanjilal PB, Leclercq PA. Composition of the seed oil of Trachyspermum ammi (L.) sprague from Northeast India. J Essential Oil Res 1998;10:588-90.
8. Sivropoulou A, Papanikolaou E, Nilolaou C, Kokkini S, Lanaras T, Arsenakis M. Antimicrobial and cytotoxic activities of origanium essential oils. J Agric Food Chem 1996;44:1202-5.
9. Vasudevan K, Vembar S, Veeraraghavan K, Haranath PS. Influence of intragastric perfusion of aqueous spice extracts on acid secretion in anesthetized albino rats. Indian J Gastroenterol: Official J Indian Soc Gastroenterol 1999;19:53-6.
10. Gilani AH, Jabeen Q, Ghayur MN, Janbaz KH, Akhtar MS. Studies on the antihypertensive, antispasmodic, bronchodilator and hepatoprotective activities of the carum copticum seed extract. J Ethnopharmacol 2005;98:127-35.
11. Ahsan SK, Shah AH, Tanira MOM, Ahmad MS, Tariq M, Ageel AM. Studies on some herbal drugs used against kidney stones in Saudi folk medicine. Fitoterapia 1990;61:435-8.
12. Nath D, Sethi N, Srivastava S, Jain AK, Srivastava R. Survey on indigenous medicinal plants used for abortion in some districts of Uttar Pradesh. Fitoterapia 1997;68:223-5.
13. Kaur H. Estrogenic activity of some herbal galactagogue constituents. Indian J Animal Nutr 1998;15:232-4.
14. Srivastava KC. Extract of a spice-omum (Trachyspermum ammi)-shows antiaggregatory effects and alters arachidonic acid metabolism in human platelets. Prostaglandins Leukotrienes Essent Fatty Acids 1988;33:1-6.
15. Thangam C, Dhananjayan R. Antiinflammatory potential of the seeds of carum copticum linn. Indian J Pharmacol 2003;35:388-91.
16. Boskabady MH, Jandaghi P, Kiani S, Hasanzadeh L. Antitussive effect of Carum copticum in guinea pigs. J Ethnopharmacol 2005;97:79-82.
17. Mathew N, Misra-Bhattacharya S, Perumal V, Muthuswamy K. Antifilarial lead molecules isolated from trachyspermum ammi. Molecules 2008;13:2156-68.
18. Ramaswamy S, Sengottuvelu S, Sherief SH, Jaikumar S, Saravanan R, Prasadkumar C, et al. Gastroprotective activity of ethanolic extract of Trachyspermum ammi fruit. Int J Pharma Bio Sci 2010;1:1-15.
19. Priestley CM, Williamson EM, Wafford KA, Sattelle DB. Thymol, a constituent of thyme essential oil, is a positive allosteric modulator of human GABAA receptors and a homo‐oligomeric GABA receptor from Drosophila melanogaster. Br J Pharmacol 2003;140:1363-72.
20. Velazhahan R, Vijayanandraj S, Vijayasamundeeswari A, Paranidharan V, Samiyappan R, Iwamoto T, et al. Detoxification of aflatoxins by seed extracts of the medicinal plant, Trachyspermum ammi (L.) Sprague ex Turrill–structural analysis and biological toxicity of degradation product of aflatoxin G1. Food Control 2010;21:719-25.
21. Anilakumar KR, Saritha V, Khanum F, Bawa A. SAmeliorative effect of ajwain extract on hexachlorocyclohexane-induced lipid peroxidation in rat liver. Food Chem Toxicol 2009;47:279-82.
22. harmala information from NPGS/GRIN [Last accessed on 17 Feb 2008.
23. Prashanth D, John S. Antibacterial activity of peganum harmala. Fitoterapia 1999;70:438-9.
24. Panda H. Book of herbs cultivation and medicinal uses. Delhi: National Institute of Industrial Research; 2000. p. 435.
25. El-Dwairi QA, Banihani SM. Histo-functional effects of Peganum harmala on male rat's spermatogenesis and fertility. Neurol Endocrinol Lett 2007;28:305-10.
26. Monsef HR, Ghobadi A, Iranshahi M, Abdollahi M. Antinociceptive effects of peganum harmala L. alkaloid extract on mouse formalin test. J Pharm Pharm Sci 2004;7:65-9.
27. M Abdolahi. Peganum harmala L. alkaloid extract on mouse formalin test. J Pharm Pharm Sci 2008;7:65–9.
28. Splettstoesser F, Bonnet U, Wiemann M, Bingmann D, Busselberg D. Modulation of voltage‐gated channel currents by harmaline and harmane. Br J Pharmacol 2005;144:52-8.
29. Azam Fatahi, Ali Ajami, Mojgan Bozorgzad, Shahram Nekoeian, Atefeh Khazeni. A comparison between culture and multiplex PCR for detection and identification of Shigella species in patients with Shigellosis from Isfahan province in 2014-2015. J Med Microbiol Infection Diseases 2015;3:23-8.
30. D Riding. Acute bacillary dysentery in khartoum province, sudan, with special reference to bacteriophage treatment: bacteriological investigation. J Hygiene 1930;30:387-401.
31. Hale TL, Keusch Gt. Shigella. In: Baron S. Editor. Medical Microbiology. 4th Edition. Galveston (Tx): University of Texas Medical Branch at Galveston: Chap2; 1996.
32. Evans, William Charles. Trease and Evans' Pharmacognosy. 11 edition. Elsevier Health Sciences; 2009.
33. Sofowora A. Recent trends in research into African medicinal plants. J Ethnopharmacol 1993;38:197-208.
34. Wayne, Pensilvania document M100-S9. Performance standards for antimicrobial susceptibility testing. Vol. 19. National Committee for Clinical Laboratory Standards (NCCLS); 1999.
35. Ishikawa T, Sega Y, Kitajima J. Water-soluble constituents of ajowan. Chem Pharm Bull 2001;49:840-4.
36. Mosa Elhady Osman, Hanadi Hassan Yassen, Justin Deng Deng, Remaz Omar Mustafa, Saad Mohamed Hussien, General phytochemical screening and antioxidant of some sudanese medicinal plants. J For Prod Ind 2014;3:292-5.
37. Prescott LMH, Arley JP, Klein D. Microbiology (International Edition). Fifth edition. Published By Mc Graw Hill Book Company; 2002. p. 809-19.
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How to Cite
Hamid, I., and I. Eltayeb. “IN VITRO SUSCEPTIBILITY OF ISOLATED SHIGELLA FLEXNERI AND SHIGELLA DYSENTERIAE TO THE ETHANOLIC EXTRACTS OF TRACHYSPERMUM AMMI AND PEGANUM HARMALA”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 11, no. 1, Jan. 2019, pp. 103-6, doi:10.22159/ijpps.2019v11i1.29411.
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