ANTI HYPERGLYCEMIC EVALUATION OF TERMINALIA CHEBULA LEAVES

  • Jayashree Dutta Department of Biotechnology, Gauhati University 781014
  • Mohan Chandra Kalita Department of Biotechnology, Gauhati University 781014

Abstract

Objective: The antihyperglycaemic potentiality of Terminalia chebula leaves has not yet been investigated thoroughly compared to its fruit counterpart. Therefore, the purpose of this study was to assess the hypoglycaemic potentiality of Terminalia chebula Retz leaves both in vitro and in vivo.


Methods: Fresh leaves of T. chebula were collected, authenticated and grounded to a fine powder. The powdered material was extracted in methanol. The hypoglycaemic potentiality of the extract was accessed in vitro using enzyme alpha-amylase and alpha-glucosidase. The antihyperglycaemic activity of the methanol extract active fraction was accessed in vitro and in vivo. The active fraction thus obtained was partially characterized using Fourier transform infrared spectroscopy (FTIR) and High-performance liquid chromatography (HPLC) analysis.


Results: The crude leave methanol extract of Terminalia chebula demonstrated 100% α glucosidase inhibition with IC50–0.956±0.342 mg/ml compared to standard drug acarbose. Oral administration of the active fraction to diabetic rats loaded with maltose significantly (P<0.05) retarded the postprandial spike of blood glucose level compared to standard drug acarbose. Partial characterization of the fraction reveals the presence of hydrosoluble tannin gallic acid.


Conclusion: The study provides an in vitro and in vivo rationale evidence of Terminalia chebula leaves to retard postprandial hyperglycemia.

Keywords: Terminalia chebula, Postprandial hyperglycemia (PPHG), α amylase, α glucosidase, Active fraction (f5)

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Author Biographies

Jayashree Dutta, Department of Biotechnology, Gauhati University 781014

Research Associate, Department of Biotechnology

Gauhati University

Mohan Chandra Kalita, Department of Biotechnology, Gauhati University 781014

Professor, Department of Biotechnology

Gauhati University

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Dutta, J., and M. C. Kalita. “ANTI HYPERGLYCEMIC EVALUATION OF TERMINALIA CHEBULA LEAVES”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 10, no. 11, Nov. 2018, pp. 43-48, doi:10.22159/ijpps.2018v10i11.28167.
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