POPULATION STRUCTURE OF KAEMPFERIA GALANGA L. FROM EASTERN INDIA
Objective: India has been a producer of a large number of aromatic medicinal plants which serves as a valuable genetic resource for future quality improvement to meet the ever-growing demand of human essential products. Thus, an urgent need arises for germplasm conservation of these high yielding varieties to help the pharmaceutical and other industries. For this understanding, the population structure is essential in order to explore their genetic identification by fingerprinting and molecular characterization.
Methods: In the present study DNA was isolated using modified Cetyl Trimethyl Ammonium Bromide (CTAB) method and Polymerase Chain Reaction (PCR) was performed according to standardized method along with its data analysis. This study was undertaken to characterize the highly medicinal Kaempferia galanga collected from 4 different populations of Odisha using the molecular markers as Random Amplified Polymorphic DNA and Inter-Simple Sequence Repeats for the first time.
Results: A dendrogram constructed through Sequential Agglomerative Hierarchical and Nested (SAHN) clustering and Unweighted Pair Group Method with Arithmetic mean (UPGMA) analysis showed an average similarity of 0.993 ranging between 0.967 to 1.000. Jaccard’s similarity coefficient of combined markers segregated the genotypes into two main clusters, 1 with six samples and the others at 0.98 similarity coefficient.
Conclusion: Hence, the molecular analysis could be further used for the identification of important novel gene present in Kaempferia galanga which can be utilized for future crop improvement as well as pharmacological activities.
2. Rahman MM, Amin MN, Ahamed T, Ali MR, Habib A. Efficient plant regeneration through somatic embryogenesis from leaf base derived callus of Kaempferia galangal L. Asian J Plant Sci 2004;3:675-8.
3. Sulaiman MR, Zakaria ZA, Duad IA, Hidayat MT. Antinociceptive and anti-inflammatory activities of the aqueous extract of Kaempferia galangal leaves in animal models. J Nat Med 2008;62:221-7.
4. Chan EWC, Lim YY, Wong SK, Lim KK, Tan SP, Lianto FS, et al. Effects of drying methods on the antioxidant properties of leaves and tea of ginger species. Food Chem 2009;113:166-72.
5. Sumazian Y, Syahida A, Hakiman M, Maziah M. Antioxidant activities, flavonoids, ascorbic acid and phenolic contents of Malaysian vegetables. J Med Plants Res 2010;4:881-90.
6. Sulaiman MR, Zakaria ZA, Duad IA, Hidayat MT. Antinociceptive and anti-inflammatory activities of the aqueous extract of Kaempferia galangal leaves in animal models. J Nat Med 2008;62:221-7.
7. Chan EWC, Lim YY, Wong SK, Lim KK, Tan SP, Lianto FS, et al. Effects of drying methods on the antioxidant properties of leaves and tea of ginger species. Food Chem 2009;1:166-72.
8. Kanjanpothy D, Kanjanapothi A, Panthong N, Lertprasertsuke T, Taesotikul C, Rujjanawate D, et al. Toxicity of crude rhizome extract of Kaempferia galanga L. (Proh Hom). J Ethnopharmacol 2004;90:359–65.
9. Wahab SI, Ain NM, Abdul AB, Elhassan MM, Ibrahim TA. Energy-dispersive X-ray microanalysis of elements content of medicinal plants used traditionally as anticancer cure. Res J Biol Sci 2009;4:547-9.
10. Shirin F, Kumar S, Mishra Y. In vitro plantlet production system for Kaempferia galanga, a rare Indian medicinal herb. Plant Cell Tiss Org Cult 2000;63:193-7.
11. Doyle JJ, Doyle JL. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 1990;19:11-5.
12. Williams JGK, Kubelik AR, Liva KJ, Rafalski JA, Tingey SV. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl Acid Res 1990;18:6531-5.
13. Zeitkiewicz E, Rafalski A, Labuda D. Genome fingerprinting by simple sequence repeat (SSR)-anchored PCR amplification. Genomics 1994;20:176-83.
14. Gherardi M, Mangin B, Goffinet B, Bonnet D, Huguet T. A method to measure genetic distance between allogamous populations of alfalfa (Medicago sativa) using RAPD molecular markers. Theor Appl Genet 1988;96:406-12.
15. Prevost A, Wilkins MJ. A new system for comparing PCR primers applied to ISSR fingerprinting of potato cultivars. Theor Appl Genet 1999;98:661-8.
16. Smith JSC, Chin ECL, Shu H, Smith OS, Wall SJ, Senior ML, et al. An evaluation of the utility of SSR loci as molecular markers in maize (Zea mays L.): comparison of data from RFLPs and pedigree. Theor Appl Genet 1997;95:163-73.
17. Ghislain M, Zhang D, Fazardo D, Huamann Z, Hismans RH. Marker-assisted sampling of the cultivated andean potato Solanum fureja collection using RAPD markers. Genetic Res Crop Evol 1999;46:547-55.
18. Jaccard P. Nouvelles recherché sur Ia distribution florale. Bull Soc Vaudense Des Sci Naturelles 1908;44:223-70.
19. Sneath PHA, Sokal RR. Numerical Taxonomy, Freeman, San Francisco, California; 1973. p. 573.
20. Rohlf FJ. NTSYS-pc Numerical taxonomy and multivariate analysis system. Version 2.02e. Exeter Software, Setauket, New York; 1997.
21. Reddy P, Sarla N, Siddiq EA. Inter-simple sequence repeat (ISSR) polymorphism and its application in plant breeding. Euphytica 2002;28:9–17.
22. Nayak S, Naik PK, Acharya LK, Mukherjee AK, Panda PC, Das P. Assessment of genetic diversity among 16 promising cultivars of ginger using cytological and molecular markers. Z Naturforsch 2005;60:485-92.
23. Sasikumar B. Genetic resources of Curcuma: diversity, characterization, and utilization. Plant Genetic Res 2005;3:230-51.
24. Kress WJ, Prince LM, Williams KJ. The phylogeny and a new classification of the gingers (Zingiberaceae): evidence from molecular data. Am J Bot 2002;89:1682–96.
25. Techaprasan J, Klinbunga S, Ngamriabsakul C, Jenjittikul T. Genetic variation of Kaempferia (Zingiberaceae) in Thailand based on chloroplast DNA (psbA-trnH and petA-psbJ) sequences. Gen Mol Res 2010;9:1957-73.
26. Preetha TS, Hemanthakumar AS, Padmesh P, Krishnan PN. Genetic uniformity analysis of cryopreserved in vitro plantlets of Kaempferia galanga L.-an endangered medicinal species in tropical Asia. Indian J Biotech 2015;14:425-8.
27. Preetha TS, Hemanthakumar AS, Krishnan PN. A comprehensive review of Kaempferia galanga L. (Zingiberaceae): a high sought medicinal plant in tropical Asia. J Med Plant Stud 2016;4:270-6.
28. Jatoi SA, Kikuchi A, Yi SS, Naing KW, Yamanka S, Wataanbe JA, et al. Use of rice SSR markers as RAPD markers for genetic diversity analysis in Zingiberaceae. Breeding Sci 2006;56:107-11.
29. Siriluck I, Ratchanok T, Worakij H, Thitamin K. Identification of 24 species of Zingiberaceae in Thailand using ISSR technique. Thai J Agric Sci 2014;1:1-6.
30. Amalia SK, Viktoria ME, Amarila M. Rapid PCR–based detection optimization of porcine DNA in the gelatin capsule shell. Int J Appl Pharm 2018;10:217-23.
31. Prabu P, Edayadulla N, Anand S. In vitro antibacterial activity and gas chromatography-mass spectrometry analysis of an ethanolic extract of leaves of Elettaria cardamomum L. maton. Asian J Pharm Clin Res 2019;12:73-5.