• Bushra Khanam Birla Institute of Technology, Mesra 835215, Ranchi, Jharkhand, India
  • Ramesh Chandra Birla Institute of Technology, Mesra, Ranchi, India


Objectives: Isolation of endophytic bacteria from the of Beta vulgaris L. for production of pigment. Characterization of isolated bacterial endophyte with respect to morphological, biochemical and molecular biological aspects which lead to their identification.

Methods: Plant materials were sterilized by standard methods. Isolation of bacterial endophyte using nutrient media was carried out. Sterility check of explants was performed using two techniques. Morphological, biochemical and molecular biology methods were employed for identification of isolated endophyte. Light microscopy and scanning electron microscopy, molecular gene sequencing was performed on endophytic isolate KC1. Phylogenetic tree was constructed to find the similarity of KC1 and other bacteria.

Results: The isolate KC1 was found acoccobaccilus, gram negative, which gave a catalase positive reaction and after molecular identification, it was designated as Serratiamarcescens strain VIT-PTS (GenBank Accession Number: KJ716445.1)

Conclusion: The results indicate that 17 endophytic bacteria are isolated out of that one bacterial isolate KC1 secretes red pigment. Based on the phylogenetic analysis of 16S rRNA gene sequences, isolate KC1 and Serratiamarcescens were in the same terminal clade. This bacterial pigment, which is expected to be prodigiosin, is promising as potential textile and food colorant.


Keywords: Endophytic bacteria, Beta valgaris L., Identification, Serratiamarcescens, Red pigment


Download data is not yet available.

Author Biography

Ramesh Chandra, Birla Institute of Technology, Mesra, Ranchi, India
Department of Bio-Engineering, Associate Professor


1. Azevedo JL, Maccheroni J, Pereira O, Ara WL. Endophytic microorganisms: a review on insect control and recent advances on tropical plants. Electron J Biotechnol 2001;3:40-65.
2. Zhang HW, Song CY, Tan RX. Biology and chemistry of endophytes. Nat Prod Rep 2006;23:753-71.
3. Bandara WM, Seneviratne G, Kulasooriya SA. Interactions among endophytic bacteria and fungi: effects and potentials. J Biosci 2006;31:645-50.
4. Bills GF, Polishook JD. Microfungi from Carpinuscaroliniana. Can J Bot Canadian 1991;69:1477-82.
5. Kushwaha K, Saini A, Saraswat P, Agarwal MK, Saxena J. Colorful world of microbes: carotenoids and their applications. Adv Biomater 2014;24:4615–24.
6. Babu S, Shenolikar IS. Health and nutritional implications of food colours. Indian J Med Res 1995;102:245–9.
7. Johnson EA, Schroeder WA. Microbial carotenoids. Adv Biochem Eng Biotechnol 1996;53:119–78.
8. Gunasekaran S, Poorniammal R. Optimization of fermentation conditions for red pigment production from Penicilliumsp. under submerged cultivation. Afr J Biotechnol 2008;7:1894-8.
9. Jiang Y, Li HB, Chen F, Hyde KD. Production potential of water-soluble Monascus red pigment by a newly isolated Penicillium sp. J Agr Sci Tech 2005;1:113-26.
10. Strobel A. Endophytes as sources of bioactive products. Microbes Infect 2003;5:535–44.
11. Melvyn G. Pigments of fungi (Macromycetes). Nat Prod Rep 2003;20:615–39.
12. Pattnaik P, Roy U, Jain P. Biocolours: new generation additives for food. Indian Food Industry 1997;16:21-32.
13. Hallmann J, Quadt-Hallmann A, Rodrıguez KR, Kloepper JW. Interactions between Meloidogyne incognita and endophytic bacteria in cotton and cucumber. J Soil Biol Biochem 1997;30:925-37.
14. Suryanarayanan TS, Wiltlinger KS, Faeth HS. Endophytic fungi associated with cacti Arizona. Mycological Res 1998;109:635-9.
15. Vega FE, Pava-Ripoll M, Buyer JS. Endophytic bacteria in Coffeaarabica L. J Basic Microbiol 2005;45:371–80.
16. Pleban S, Ingel F, Chet I. Control of Rhizoctoniasolani and Sclerotiumrolfsiiin the greenhouse using endophytic Bacillus spp. Eur J Plant Pathol 1995;101:665-72.
17. McInroy JA, Kloepper JW. Studies on indigenous endophytic bacteria of sweet cornand cotton. In: O’Gara F, Dowling DN, Boesten B. eds. Molecular ecology of rhizosphere microorganisms. Weinheim VC. Germany; 1994. p.19–28.
18. Harley JP, Prescott L. Laboratory Exercises in Microbiology. Fourth Edition. McGraw-Hill; 1999.
19. Ho WH, Ranghoo VM, Hyde KD, Hodgkiss IJ. Ultrastructural study in Annulatascus hongkongensiss pnov., a freshwater ascomycete. Mycologia 1999;91:885–92.
20. Felsenstein J. Phylogensis inference package (PHYLIP), version 3.69. Department of genome sciences and Department of biology, university of Washington, seattle, WA, USA; 2008.
21. Saitou N, Nei M. The neighbor-joining method: A new method for reconstructing phylogenetic trees. J Mol Evol 1987;4:406-25.
22. Felsenstein J. Confidence limits on phylogenies: An approach using the bootstrap. J Mol Evol 1985;39:783-91.
23. Kimura M. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980;16:111-20.
24. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. J Mol Evol 2011;28:2731-9.
25. Baayen RP, Bonants PJM, Verkley G. Nonpathogenic isolates of the citrus black spot fungus, Guignardiacitricarpa, identified as a cosmopolitan endophytes of woody plants, G. magniferae (Phylostidacapilalensis). Phytopathol 2002;92:4644-477.
26. Sameer ASM, Kristian FN, Thomas OL, Jens CF, Anne SM, Ulf T. Exploring fungal biodiversity for the production of water soluble pigments as potential natural food colourants. Curr Opin Biotechnol 2005;16:231-8.
1036 Views | 2068 Downloads
How to Cite
Khanam, B., and R. Chandra. “ISOLATION AND IDENTIFICATION OF ENDOPHYTIC BACTERIA PRODUCING BRIGHT RED PIGMENT FROM THE DYE YIELDING PLANT BETA VULGARIS L”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 7, no. 5, Mar. 2015, pp. 220-4,
Original Article(s)