ANALYSIS OF PROTEIN ISOLATE FROM QUINOA (CHENOPODIUM QUINOA WILLD)

Authors

Abstract

ABSTRACT
Objective: The aim of this study was to obtain protein isolate from quinoa using alkaline pH at different pHs of precipitation and to analyze protein
isolate with electrophoresis.
Methods: Quinoa protein isolates were obtained using isoelectric precipitation method at different pHs. Proteins were analyzed using electrophoresis
native-polyacrylamide gel electrophoresis (PAGE) and sodium dodecyl sulfate - PAGE.
Results: A yield of 6.29% of protein isolate of defatted quinoa at pH 4.0 was obtained. The content of protein isolate was higher than 64% in all
pH assays. Globulins and albumins in protein isolate at different pHs were observed. One band near 130 kDa was found. A band with MW 60 kDa
corresponding to 7S globulin was found. The bands, MW 33-36 kDa and MW 20-22 kDa, correspond to 11S globulin. Bands less to 15.4 kDa correspond
to albumins.
Conclusions: Quinoa is a good source of proteins. Globulins and albumins were identified in the quinoa protein isolate.
Keywords: Quinoa, Globulins, Albumins, Polypeptides, Protein isolate.

Author Biography

CARRILLO W, Universidad Técnica de Ambato

Laboratorio de alimentos funcionales. Facultad de Ciencia e Ingeería en Aliementos

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Published

2016-03-01

How to Cite

A, T., C. C, V. R, and C. . W. “ANALYSIS OF PROTEIN ISOLATE FROM QUINOA (CHENOPODIUM QUINOA WILLD)”. Asian Journal of Pharmaceutical and Clinical Research, vol. 9, no. 2, Mar. 2016, pp. 332-4, https://innovareacademics.in/journals/index.php/ajpcr/article/view/10773.

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