ANALYSIS OF PROTEIN ISOLATE FROM QUINOA (CHENOPODIUM QUINOA WILLD)

Abstract

ABSTRACT
Objective: The aim of this study was to obtain protein isolate from quinoa using alkaline pH at different pHs of precipitation and to analyze protein
isolate with electrophoresis.
Methods: Quinoa protein isolates were obtained using isoelectric precipitation method at different pHs. Proteins were analyzed using electrophoresis
native-polyacrylamide gel electrophoresis (PAGE) and sodium dodecyl sulfate - PAGE.
Results: A yield of 6.29% of protein isolate of defatted quinoa at pH 4.0 was obtained. The content of protein isolate was higher than 64% in all
pH assays. Globulins and albumins in protein isolate at different pHs were observed. One band near 130 kDa was found. A band with MW 60 kDa
corresponding to 7S globulin was found. The bands, MW 33-36 kDa and MW 20-22 kDa, correspond to 11S globulin. Bands less to 15.4 kDa correspond
to albumins.
Conclusions: Quinoa is a good source of proteins. Globulins and albumins were identified in the quinoa protein isolate.
Keywords: Quinoa, Globulins, Albumins, Polypeptides, Protein isolate.

Author Biography

Wilman Carrillo, Universidad Técnica de Ambato
Laboratorio de alimentos funcionales. Facultad de Ciencia e Ingeería en Aliementos

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How to Cite
Carrillo, W., C. C, T. A, and V. R. “ANALYSIS OF PROTEIN ISOLATE FROM QUINOA (CHENOPODIUM QUINOA WILLD)”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 9, no. 2, Mar. 2016, pp. 332-4, https://innovareacademics.in/journals/index.php/ajpcr/article/view/10773.
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