ISOLATION OF HEN EGG WHITE LYSOZYME BY CATION EXCHANGE CHROMATOGRAPHY, ANALYSIS OF ITS DIGESTIBILITY AND EVALUATION OF THE INHIBITION LIPID PEROXIDATION IN THE ZEBRAFISH MODEL

  • Wilman Carrillo Universidad Técnica de Ambato http://orcid.org/0000-0002-7609-2939
  • Jessica Tubón Universidad Técnica de Ambato
  • Rubén Vilcacundo Universidad Técnica de Ambato

Abstract

Objective: The aim of this study was to separate and identify lysozyme using cation exchange chromatography, evaluate the protein digestibility and
analyze the inhibition lipid peroxidation in the zebrafish model.
Methods: Hen egg white lysozyme was isolate with cation exchange chromatography. Residual muramidase activity was evaluated with the
spectrophotometric method. Isolate lysozyme (ILZ) and hydrolysates were analyzed with sodium dodecyl sulfate-polyacrylamide gel electrophoresis
(SDS-PAGE) electrophoresis and reversed-phase high-performance liquid chromatography (RP-HPLC).
Results: To identify the protein, the sample was isolated with cationic exchange chromatography, sample was analyzed using RP-HPLC and SDS-PAGE
confirming that the fraction was hen lysozyme egg white. The enzymatic activity of the isolated protein was normal compared to the commercial
lysozyme activity. Hydrolysates had no muramidase activity and were able to inhibit lipid peroxidation in zebrafish larvae.
Conclusions: Cation exchange chromatography is a good method to ILZ from egg white. Hydrolysates of lysozyme were effective to inhibit lipid
peroxidation in zebrafish model.
Keywords: Lysozyme, Cation exchange chromatography, Enzymatic hydrolysis, Muramidase activity.

Author Biographies

Wilman Carrillo, Universidad Técnica de Ambato
Facultad de Ciencia e Ingeería en Aliementos
Jessica Tubón, Universidad Técnica de Ambato
Facultad de Ciencia e Ingenería en Alimentos
Rubén Vilcacundo, Universidad Técnica de Ambato
Facultad de Ciencia e Ingenría en Alimentos

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How to Cite
Carrillo, W., TubónJ., and R. Vilcacundo. “ISOLATION OF HEN EGG WHITE LYSOZYME BY CATION EXCHANGE CHROMATOGRAPHY, ANALYSIS OF ITS DIGESTIBILITY AND EVALUATION OF THE INHIBITION LIPID PEROXIDATION IN THE ZEBRAFISH MODEL”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 9, no. 3, May 2016, pp. 345-9, https://innovareacademics.in/journals/index.php/ajpcr/article/view/11462.
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