ISOLATION OF PROTEINS FROM SACHA INCHI (PLUKENETIA VOLUBILIS L.) IN PRESENCE OF WATER AND SALT

  • Wilman Carrillo Universidad Técnica de Ambato http://orcid.org/0000-0002-7609-2939
  • María Fernanda Quinteros Universidad Técnica de Ambato
  • Cecilia Carpio Universidad Técnica de Ambato
  • Ruben Vilcacundo Universidad Técnica de Ambato

Abstract

Objective: The aim of this study was to obtain protein isolate from sacha inchi using alkaline pH at different pHs of precipitation with water and salt
and to analyze protein isolate with electrophoresis.
Methods: Sacha inchi protein isolates were obtained using isoelectric precipitation method at different pHs. Proteins were analyzed using
electrophoresis native-polyacrylamide gel electrophoresis (PAGE), one-dimensional, two-dimensional-sodium dodecyl sulfate-PAGE.
Results: A yield of 20.88% of protein isolate of defatted sacha inchi flour at pH 4.0 with a 75.31% of protein was obtained. The yield of protein isolate
using water and salt was similar. Polypeptides profile is between 14 and 70 kDa.
Conclusions: Sacha inchi seed is a good source of proteins. Globulins and albumins were identified in the sacha inchi protein isolate in the presence
of water and salt.
Keywords: Albumins, Globulins, Proteins, Protein isolate, Sacha inchi.

Author Biographies

Wilman Carrillo, Universidad Técnica de Ambato
Laboratorio de Alimentos Funcionales. Facultad de Ciencia e Ingeería en Aliementos
María Fernanda Quinteros, Universidad Técnica de Ambato
Laboratorio de Alimentos Funcionales. Facultad de Ciencia e Ingeniería en Alimentos.
Cecilia Carpio, Universidad Técnica de Ambato
Laboratorio de Alimentos Funcionales. Facultad de Ciencia e Ingeniería en Alimentos
Ruben Vilcacundo, Universidad Técnica de Ambato
Laboratorio de Alimentos Funcionales. Facultad de Ciencia e Ingnería en Alimentos

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How to Cite
Carrillo, W., M. F. Quinteros, C. Carpio, and R. Vilcacundo. “ISOLATION OF PROTEINS FROM SACHA INCHI (PLUKENETIA VOLUBILIS L.) IN PRESENCE OF WATER AND SALT”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 9, no. 3, May 2016, pp. 193-6, https://innovareacademics.in/journals/index.php/ajpcr/article/view/11007.
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