PRELIMINARY PHYTOCHEMICAL ANALYSIS AND CYTOTOXICITY POTENTIAL OF PINEAPPLE EXTRACT ON ORAL CANCER CELL LINES
Objective: This study aims at performing a preliminary phytochemical analysis to evaluate the phytochemical composition of pineapple extract and
its cytotoxicity potential on oral cancer cell lines.
Methods: Preliminary phytochemical analysis of pineapple extract was done, 3-(4, 5-Dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide
assay for evaluating the cytotoxicity potential of the extract on oral cancer cell lines was performed.
Results: Phytoconstituents such as flavonoids, coumarins, and phenols were present in the pineapple extract. The extract also exhibited increased
cytotoxicity with increased concentration.
Conclusion: This study is conducted to see if pineapple extract is effective in treating oral cancer in a natural way instead of harmful treatments.
Keywords: Cytotoxicity, Pineapple extract, Anticancer drug.
1. Riss TL, Moravec RA. Use of multiple assay endpoints to investigate
the effects of incubation time, dose of toxin, and plating density in cellbased
cytotoxicity assays. Assay
Drug Dev Technol
2. Decker T, Lohmann-Matthes ML. A quick and simple method for
the quantitation of lactate dehydrogenase release in measurements
of cellular cytotoxicity and tumor necrosis factor (TNF) activity.
J Immunol Methods 1988;115(1):61-9.
3. Niles AL, Moravec RA, Eric Hesselberth P, Scurria MA, Daily WJ,
Riss TL. A homogeneous assay to measure live and dead cells in the
same sample by detecting different protease markers. Anal Biochem
4. Fan F, Wood KV. Bioluminescent assays for high-throughput screening.
Assay Drug Dev Technol 2007;5(1):127-36.
5. Phytotoxicity - Pesticides. Province of British Columbia, 2007.
6. Krogmeier MJ, McCarty GW, Bremner JM. Phytotoxicity of foliar-
applied urea. Proc Natl Acad Sci U S A 1989;86(21):8189-91.
7. Krstic D, Bjelic D, Nikezic D, Djalovic I. Aluminium in Acid Soils:
Chemistry, Toxicity and Impact on Maize Plants. Rijeka, Croatia:
INTECH Open Access; 2012.
8. Buczacki S, Harris K. Disorders. Pests, Diseases & Disorders of Garden
Plants. London: Collins; 1998. p. 609.
9. Sofowora A. Screening plants for bioactive agents. Medicinal Plants
and Traditional Medicinal in Africa. 2
ed. Ibadan, Nigeria: Spectrum
Books Ltd., Sunshine House; 1993. p. 134-56.
10. Harborne JB. Phytochemical Methods: A Guide to Modern Techniques
of Plant Analysis. 3
ed. New York: Chapman and Hall; 1973. p. 279.
11. Smolenski SJ, Silinis H, Farnsworth NR. Alkaloid screening. V. Lloydia
12. Kapoor LD, Singh A, Kapoor SL, Srivastava SN. Survey of
Indian plants for saponins, alkaloids and flavonoids. I. Lloydia
13. Jana S, Shekhawat GS. Phytochemical analysis and antibacterial
screening of in vivo and in vitro extracts of Indian medicinal herbs:
Anethum graveolens. Res J Med Plants 2010;4(4):206-12.
14. Ayoola GA, Coker HA, Adesegun SA, Adepoju-Bello AA, Obaweya K,
Ezennia EC, et al. Phytochemical screening and antioxidant activities
of some selected medicinal plants used for malaria therapy in
Southwestern Nigeria. Trop J Pharm Res 2008;7(3):1019-24.
15. Sureshkumar CA, Varadharajan R, Muthumani P, Meera R, Devi P,
Kameswari B. Pharmacognostic and preliminary phytochemical
investigations on the stem of Saccharum spontaneum. J Pharm Sci Res
16. Boxi M, Rajesh Y, Rajakumar V, Praveen B, Mangamma K. Extraction,
phytochemical screening and in-vitro evaluation of anti-oxidant
properties of commicarpuschinesis (aqueous leaf extract). Int J Pharm
Bio Sci 2010;1(4):975-6299.
17. Kolawole OM, Oguntoye SO, Agbede O, Olayemi AB. Studies on the
efficacy of Bridelia ferruginea Benth. Bark extract in reducing the
coliform load and BOD of domestic waste water. Ethnobotanical Lealf
18. Mosmann T. Rapid colorimetric assay for cellular growth and survival:
Application to proliferation and cytotoxicity assays. J Immunol
19. Rajeshwari A. Evaluarion of phytochemical constituents, quantitative
analysis and antimicrobial efficacy of potential herbs
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