CYTOTOXICITY OF STRAWBERRY EXTRACT ON ORAL CANCER CELL LINE
Keywords:Apoptosis, Berries, Cancer, Cytotoxic effect, (3-[4, 5-Dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide), Neutral red
Objective: Berries including strawberry may have beneficial effects against oxidative stress-mediated diseases such as cancer. The purpose of the present study was to investigate the cytotoxic effects of strawberry extract on oral cancer cell line.
Methods: Strawberry methanolic extract (SBE) was prepared, and cytotoxic activity of different concentration of SBE on KB cell lines was determined by (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay and neutral red dye incorporation test.
Results: Strawberry extract exhibits cytotoxic activity over the oral cancer cell lines. On administration of about 100 Î¼g/ml of strawberry extract, about 50% of cell viability could be observed and assessed from the cell lines.
Conclusion: Strawberries have a cytotoxic effect on oral cancer cell line due to the presence of anticancer constituents in the berries. These berries can be used as a natural medicine for cancer sufferers.
Packyanathan JS, Gayathri R, Vishnupriya V. Preliminary phytochemical analysis and cytotoxicity potential of Bacopa monnieri on oral cancer cell lines. Int J Pharm Sci Rev Res 2016;39:4-8.
Rahman M, Islam SN. Effect of serum antioxidants (vitamin E, C and A) in lung cancer patients. Int J Pharm Pharm Sci 2014;6:578-80.
Weisburger JH. Mechanisms of action of antioxidants as exemplified in vegetables, tomatoes and tea. Food Chem Toxicol 1999;37:943-8.
Fuchs-Tarlovsky V. Role of antioxidants in cancer therapy. Nutrition 2013;29:1521.
Masters RW. Animal cell culture, cytotoxicity and viability assays. 3rd ed. Oxford: Oxford University; 2000. p. 202-203.
Tau sigma SJ, Batkin S. Bromelain, the enzyme complex of pineapple (Ananas comosus) and its clinical application. An update. J Ethnopharmacol 1988;22:191-203.
Kresty LA, Morse MA, Morgan C, Carlton PS, Lu J, Gupta A, et al. Chemoprevention of esophageal tumorigenesis by dietary administration of lyophilized black raspberries. Cancer Res 2001;61:6112-9.
Eberhardt MV, Lee CY, Liu RH. Antioxidant activity of fresh apples. Nature 2000;405:903-4.
Sun J, Chu YF, Wu XZ, Liu RH. Antioxidant and anti proliferative activities of vegetables. J Agric Food Chem 2002;50:6910-16.
Steinmetz KA, Potter JD. Vegetables, fruit and cancer. II mechanisms. Cancer Causes Control 1991;2:427-42.
Willett WC. Diet, nutrition, and avoidable cancer. Environ Health Perspect 1995;103:165-70.
Block G. Fruit, vegetables and cancer prevention: A review ofthe epidemiological evidence. Nutr Cancer 1992;18:1-29.
Ames BN. Endogenous mutagens and the causes of aging and cancer. Mutat Res 1991;250:3-16.
World Cancer Research Fund/American Institute for Cancer Research. Food, Nutrition, Physical Activity, and the Prevention of Cancer: A Global Perspective. AICR, Washington, DC; 2007.
Wedge DE, Meepagala KM, Magee JB, Smith SH, Huang G, Larcom LL. Anticarcinogenic activity of strawberry, blueberry, and raspberry extracts to breast and cervical cancer cells. J Med Food 2004;4:49-51.
Fotakis G, Timbrell JA. In vitro cytotoxicity assays: Comparison of LDH, neutral red, MTT and protein assay in hepatoma cell lines following exposure to cadmium chloride. Toxicol Lett 2006;160:171-7.
Menon A, Vishnupriya V, Gayathri R. Preliminary phytochemical analysis and cytotoxicity potential of pineapple extract on oral cancer cell lines. Asian J Pharm Clin Res 2016;9:140-3.
Chen T, Rose ME, Hwang H, Nines RG, Stoner GD. Black raspberries inhibit N-nitroso methylbenzylamine (NMBA)-induced angiogenesis in rat esophagus parallel to the suppression of COX-2 and iNOS. Carcinogenesis 2006;27:2301-7.
Hong JY, Song SH, Park HJ, Cho YJ, Pyee JH, Lee SK. Antioxidant, ant inflammatory, and ant proliferative activities of strawberry extracts. Biomol Ther 2008;16:286-92.
Carlton PS, Kresty LA, Siglin JC, Morse MA, Lu J, Morgan C, et al. Inhibition of N-nitro so methyl benzyl amine-induced tumorigenesis in the rat esophagus by dietary freeze-dried strawberries. Carcinogenesis 2001;22:441-6.
Prashanth NV, Dilip C, Dev KT, Augustine L, Saraswathi R. Research Article Evaluation of cytotoxic, in vitro antioxidant activities of Ipomea batatas. Int J Pharm Pharm Sci 2010;2:91-2.
Rao SJ, Ramesh CK, Mahmood R, Jamuna KS, Prabhakar BT. Antitumor activity of two species of mulberry against eat cell lines in mice. World J Pharm Res 2015;4:1934-43.
Zhang Y, Seeram NP, Lee R, Feng L, Heber D. Isolation and identification of strawberry phenolics with antioxidant and human cancer cell antiproliferative properties. J Agric Food Chem 2008;56:670-5.
Seeram NP, Lee R, Scheuller HS, Heber D. Identification of phenolic compounds in strawberries by liquid chromatography electrospray ionization mass spectroscopy. Food Chem 2006;97:1-11.
Xue H, Aziz RM, Sun N, Cassady JM, Kamendulis LM, Xu Y, et al. Inhibition of cellular transformation by berry extracts. Carcinogenesis 2001;22:351-6.
Arfan M, Khan R, Rybarczyk A, Amarowicz R. Antioxidant activity of mulberry fruit extracts. Int J Mol Sci 2012;13:2472-80.
Stoner GD, Wang LS, Casto BC. Laboratory and clinical studies of cancer chemoprevention by antioxidants in berries. Carcinogenesis 2008;29:1665-74.
Hasan S, Elongovan S. Conventional and advanced diagnostic aids in oral cancer screening-the journey so far. Int J Pharm Pharm Sci 2015;7:29-33.
Umesh TG. In vitro antioxidant potential, free radical scavenging and cytotoxic activity of Simarouba gluaca leaves. Int J Pharm Pharmaceu Sci 2015;7:411-16.
Tsao R. Chemistry and biochemistry of dietary polyphenols. Nutrients 2010;2:1231-46.
Gug K. Physiological and whitening effects of Morus alba extracts. J Chosun Nat Sci 2012;5:46-52.
How to Cite
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.