FLOATING GASTRORETENTIVE OF AMOXICILLIN USING HARD ALGINATE CAPSULES AND ITS ANTIBACTERIAL ACTIVITIES

  • Anayanti Arianto Department of Pharmaceutical Technology, Faculty of Pharmacy, Nanomedicine Centre, University of Sumatera Utara, Indonesia.
  • Hakim Bangun Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Sumatera Utara.
  • Ade Yohana Department of Pharmaceutical Technology, Faculty of Pharmacy, Nanomedicine Centre, University of Sumatera Utara, Indonesia.
  • Jansen Silalahi Department of Pharmaceutical Technology, Faculty of Pharmacy, Nanomedicine Centre, University of Sumatera Utara, Indonesia.

Abstract

Objective: The aim of this study was to formulate floating gastroretentive of amoxicillin  using hard alginate capsules shell and to evaluate antibacterial activities of floating gastroretentive of amoxicillin.

Methods: Alginate capsules shell were made by using sodium alginate 80-120 cP. Amoxicillin was prepared in solid dispersion to obtain sustained release requirement for 12 hours. Solid dispersion was prepared by solvent method using polyvinylpyrrolidone (PVP) K30. The solid dispersion was characterized by  X-ray diffraction and FTIR analysis.  The drug release test was carried out by using USP paddle method in simulated gastric fluid. Concentrations of amoxicillin were determined by using spectrophotometer UV at 272 nm. The antibacterial activity of  aliquots  dissolution were assessed by using agar plate diffusion method against Staphylococcus aureus and Escherichia coli as bacterial model.

Results: The alginate capsules shell were made from sodium alginate 80-120 cP with size 0.  The dissolution test results showed that amoxicillin in the form of solid dispersions with weight  ratio amoxicillin with PVP K30 was 1:1 provided sustained release of amoxicillin during 12 hours, while the release of amoxicillin without solid dispersion was too slow.   The floating lag time was 0 minute and floating time was more than 12 hours. The X-ray diffraction pattern of amoxicillin solid dispersion had amorphous shape. Antibacterial activity test showed that the dissolution aliquots of amoxicillin solid dispersion were effective against  Staphylococcus aureus and Escherichia coli.

Conclusions: Based on the results of this study, it is concluded that the alginate capsules shell can be used for preparation of floating gastroretentive of amoxicillin using amoxicillin solid dispersion and the dissolution aliqouts give antibacterial effect.

 

Keywords: Floating, gastroretentive, alginate capsule, amoxicillin, solid dispersion, release, antibacterial

References

REFERENCES

1. Sukandar YE, Andrajati R, Setiadi P, Kusnandar. ISO Pharmacotherapy. Jakarta: PT. ISFI; 2008.
2. Rani AA, Fauzi. Gastric Ulcer. In: Sudoyo AW, Setiyohadi B, Alwi I, Simadibrata M, Setiati S. Internal Medicine Text book. 5th ed. Jakarta: Publisher Center of Internal Medicine; 2009.
3. Kaur SP, Rekha R, Sanju N. Amoxicilin: A broad spectrum antibiotic. International Journal of Pharmaceutical Sciences 2011; 3(3): 30-37.
4. Murakami K, Okimoto T, Kodama M, Sato R, Miyajima H, Ono M. Comparison of amoxicillin, metronidazole plus famotidine or lansoprazole for amoxicillin, clarithromycin, proton pump inhibitor treatment failures for Helicobacter pylori infection. Helicobacter 2006; 11(5):436-40.
5. Cuna M, Alonso MJ. Torres D. Preparation and in vivo evaluation of mucoadhesive microparticles containing amoxicillin–resin complexes for drug delivery to the gastric mucosa. Eur. J. Pharm. Biopharm 2001; 51(3): 199–205.
6. Shah S. Qaqish R. Patel V. Amiji M. Evaluation of the factors influencing stomach-specific delivery of antibacterial agents for Helicobacter pylori infection. J.Pharm. Pharmacol 1999; 51(6): 667-72.
7. Boyan B. Hooper J. Parisot J. Principles of assessing bacterial, susceptibility to antibiotics using the agar diffusion method. Journal of Antimicrobial Chemotherapy 2008; 61: 1295–1301.
8. Emara LH. Aya RA. Ahmed AE. Rania MB. Nesrin FT. Nadia MM. In vitro release and evaluation of gastroretentive amoxicillin floating tablets employing a specific design of the flow-through cell. Dissolution Technologies 2013; 20(1): 27-34.
9. Gopalakrishnan. Chenthilnathan. Floating drug delivery system: a review. Journal of Pharmaceutical Science and Technology 2011; 3(2): 548-54.
10. Draget KI. Smidsrod O. Gudmund S. Alginate from Algae. Weinheim: Wiley Vch Verlag GmbH and Co; 2005.
11. Aryana. Sinurat D. Ervina I. Bangun H. Formulation of alginate based metronidazole periodontal gel. Asian Journal Pharmaceutical and Clinical research 2014; 7(1): 224-27.
12. Mariadi. Bangun H. Karsono. Formulation and in vitro evaluation of gastroretentive drug delivery system of antacids using alginate-chitosan films. International J. PharmTech Research 2015; 8(9): 1-12.
13. Adliani N. Bangun H. Karsono. Preparation and evaluation of floating-mucoadhesive alginate beads as gastroretentive drug delivery system of antacids. International J. PharmTech Research 2016; 9(5): 212-22.
14. Bangun H. Simanjuntak MT. Tarigan P. Ismanelly T. Preparation and characterization of gastric resistant alginate capsules. Media Farmasi 2005; 13(1): 70-79.
15. Murthy KS. Ghebre SI. Current prespectives on the solid dissolution stability of solid oral dosage forms. Journal of Pharmaceutical Sciences 1993; 82(2): 113-26.
16. Bangun H. Preparation, physical properties, and application of hard alginate capsules. Proceeding of International Seminar on Individualized Pharmaceutics for Optimized Drug Delivery, College of Pharmacy, Seoul National University 2012.
17. Kaewnopparat N. Kaewnopparat S. Jangwang A. Maneenaun D. Chuchome T. Panichayupakaranant P. Increased solubility, dissolution, and physicochemical studies of curcumin-polyvinylpyrrolidine K-30 solid dispersion. World Academic of Science, Engeneering, and Technology 2009; (3): 225-30.
Statistics
435 Views | 413 Downloads
Citatons
How to Cite
Arianto, A., H. Bangun, A. Yohana, and J. Silalahi. “FLOATING GASTRORETENTIVE OF AMOXICILLIN USING HARD ALGINATE CAPSULES AND ITS ANTIBACTERIAL ACTIVITIES”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, no. 5, May 2017, pp. 414-20, doi:10.22159/10.22159/ajpcr.2017.v10i5.17467.
Section
Original Article(s)