ANTIOXIDANT ACTIVITY OF ALKALOID FRACTIONS OF LITSEA CUBEBA LOUR. FRUITS
Keywords:Antioxidant, Litsea cubeba, Fruits, Alkaloid, Fractions
Â Objective: The oxidation induced by free radicals results in many degenerative disease. The purpose of this study is to determine antioxidant activities of alkaloid fractions of Litsea cubeba (Lour.) fruit.
Methods: L. cubeba Lour. was extracted by maceration. Ethanol extract was fractionated with liquid-liquid extraction using n-hexane and chloroform at pH 3, 7, 9, and 11 to obtained alkaloid fractions. Antioxidant activity for extract and fractions was determined with 1,1-diphenyl-2-picrylhydrazyl.
Results: The IC50 of extract and fractions was 219.43Â±0.43, 242.97Â±0.93, 92.38Â±0.17, 40.84Â±0.04, 103.83Â±3.29, and 103.75Â±0.42 Î¼g/mL, respectively.
Conclusion: The results reveal that alkaloid fractions of L. cubeba fruits have very strong antioxidant potential. Our further study is to isolate the alkaloid compounds.
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