TOTAL PHENOLIC AND FLAVONOID CONTENTS AND ANTIOXIDANT ACTIVITY OF ETHANOL FRACTION OF PICRIA FEL-TERRAE (LOUR.) HERBS


Panal Sitorus, Poppy Anjelisazaitun Hasibuan, Denny Satria

Abstract


Objective: To evaluate total phenolic, flavonoid content (TFC and TPC) andantioxidant activity of ethyl acetate fraction (ETF) of Picria fel-terrae Lour. herbs.

Methods: TPC and TFC in ETF were determined by Folin–Ciocalteu and colorimetric methods, and antioxidant activity was determined by 1,1-diphenyl- 2-picrylhydrazyl (DPPH) method.

Results: ETF was found to contain low levels of phenolic (17.71±1.26 mg gallic acid equivalent/g), total flavonoid (14.43±0.03 mg quercetin equivalents/g). Antioxidant activity from DPPH assay was measured as inhibitory concentration 25.72±0.13 μg/mL.

Conclusions: The results reveal that ETF of P. fel-terrae Lour. herbs has strong antioxidant activity. Our further study is to the isolation of compounds which responsible for antioxidant components.


Keywords


Phenolic, Flavonoid, Antioxidant, P. fel-terrae Lour., Ethanol, Herbs.

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References


Kinnula VL, Crapo JD. Superoxide dismutases in malignant cells and human tumors. Free Radic Biol Med 2004;36(6):718-44.

Jamuna S, Pulsamy S, Karthika K. Screening of in vitro antioxidant activity of methanolic leaf and root extracts of Hypochaeris radicata L. (Asteraceae). J Appl Pharm Sci 2012;2(7):149-54.

Nagmoti DM, Khatri DK, Juvekar PR, Juvekar AR. Antioxidant activity free radical-scavenging potential of Pithecellobium dulce Benth seed extracts. Free Radic Antioxid 2012;2:37-43.

Rackova L, Oblozinsky M, Kostalova D, Kettman V, Bezakova L. Free radical scavenging activityand lipoxygenase inhibition of Mahonia aquifolium extract and isoquinoline alkaloids. J Inflam (Lond) 2007;4:15.

Rosidah, Yam MF, Sadikun A, Asmawi MZ. Antioxidant potential of Gyunura procumbens. Pharm Bio 2008;46(9):616-25.

Yang YC, Lu FH, Wu JS, Wu CH, Chang CJ. The protective effect of habitual tea consumption on hypertension. Arch Intern Med 2004;164(14):1534-40.

Anggraeni R, Hadisahputra S, Silalahi J, Satria D. Combinational effects of ethylacetate extract of Zanthoxylum acanthopodium DC. With doxorubicin on T47D breast cancer cells. Int J PharmTech Res 2014;6(7):2032-5.

Barnett GC, Shah M, Redman K, Easton DF, Ponder BA, Pharoah PD. Risk factors for the incidence of breast cancer: Do they affect survival from the disease? J Clin Oncol 2008;26(20):3310-6.

Perry LM. Medicinal Plants of East and Southeast Asia. London: The MIT Press; 1980.

Dalimunthe A, Urip H, Rosidah, Pandapotan NM. Evaluation of diuretic activity of Picria fel-terrae (Lour.) Leaves extracts. Asian J Pharm Clin Res 2015;8(4):204-5.

Huang Y, Cimanga K, Lasure A, Van Poel B, Pieters L, Berghe- Vanden D. Biological activities of Picria fel-terrae Lour. Pharm World Sci 1994;16(6):18.

Thuan ND, Ha do T, Thuong PT, Na MK, Lee JP, Lee JH, et al. A phenylpropanoid glycoside with antioxidant activity from Picria tel-ferae. Arch Pharm Res 2007;30(9):1062-6.

Zhong SQ, Zhang BN, Huang FX. An anti-tumor herb Cucao. China: ChinaTraditionally Herb Drugs Letter; 1979.

Zou JM, Wang LS, Niu XM, Sun HD, Guo YJ. Phenylethanoid glycosides from Picria felterrae Lour. J Integ Plant Bio 2005;47:632-6.

Harfina F, Bahri S, Saragih A. Pengaruh serbuk daun puguntano (Curanga fel-terrae Merr.) Pada pasien diabetes mellitus. J Pharm Pharmacol 2012;1(2):112-8.

Sitorus P, Harahap U, Pandapotan M, Barus T. Isolation of β-sitosterol from n-hexane of Picria fel-terrae Lour. Leave and study of its antidiabetic effect in alloxan induced diabetic mice. Int J Pharmtech Res 2014;6(1):137-41.

Sihotang YM, Silalahi J, Hadisahputra H, Hasibuan PA, Satria D. Cardioprotective effect of ethylacetate extract of poguntano (Picria fel-terrae Lour.) Against doxorubicin-induced cardiotoxicity in rats. Int J Pharm Clin Res 2016;8:466-70.

Patilaya P, Dadang IH. Preliminary study on the anthelmintic activity of the leaf ethanolic extract of Indonesian Curanga fel-terrae (Lour.) Merr. Int J Pharmtech Res 2015;8(3):347-51.

Harahap U, Husori DI, Marianne, Yuliasmi S, Patilaya P, Laila L, et al. Inhibitory effect of ethanolic extract of Curanga fel-terrae (pugun tano) leaves on acetylcholine muscarinic-3 receptors induced on isolated guinea pig tracheal. Asian J Pharm Clin Res 2017;10(1):95-8.

Zeng J, Pan X, Yang K, Wei Z, Chen C. ???. Chin Med Her 2010;7:27-9.

Satria D, Furqan M, Hadisahputra S, Rosidah. Combinational effects of ethylacetate extract of Picria fel-terrae Lour. And doxorubicin on T47D breast cancer cells. Int J Pharm Pharm Sci 2015;7:73-6.

Lestari P. Efek Kombinasi Ekstrak Aktif Daun Poguntano (Picria fel-terrae Lour.) Dengan Doksorubisin Terhadap sel Kanker Payudara Secara in vitro. Thesis. Medan. Faculty of Pharmacy. University of Sumatera Utara; 2013.

Hasibuan, PA, Denny S, Urip H, Jansen S. In silico analysis of Picfeltarraenin IA and IB as potential PI3K and EGFR inhibitor. Pharm Chem 2016;8(19):666-70.

Hasibuan PA, Jessy C, Denny S. Combination effect of ethylacetate extracts of Plectranthus ambonicius (Lour.) Spreng. With doxorubicin againts T47D breast cancer cells. Int J Pharm Pharm Sci 2015;7(10):155-9.

Settharaksa S, Madaka F, Chakree K, Charoenchai L. Total phenolic and flavonoid contents and antioxidant properties of Thai traditional herbal. Int J Pharm Pharm Sci 2014;6(9):564-6. 26. Diab KA, Shafik RE, Yasuda S. In vitro antioxidant and antiproliferative activities of novel orange peel extract and it’s fractions on leukemia HL-60 cells. Asian Pac J Cancer Prev 2015;16(16):7053-60.

Jebitta R, Allwin J. Antioxidant activity, total phenolic, flavonoid, and anthocyanin contents of Jamun (Syzygium cumini) pulp powder. Asian J Pharm Clin Res 2016;9:361-3.

Cicco N, Lanorte MT, Paraggio M, Viggianoa M, Lattanzio V. A reproducible, rapid, and inexpensive folin-ciocalteu micro-method in determining phenolies of plant methanol extracts. Microchem J 2009;91(1):107-10.

Shahidi F, Wanasundara PK. Phenolic antioxidants. Crit Rev Food Sci Nutr 1992;32(1):67-103.

Hatano T, Edamatsu R, Mori A. Effect of interaction of tannins and related poyphenols on superoxide anion radical and on DPPH radical. Chem Pharm Bull 1989;37:2016-21.

Umamaheswari M, Chatterjee TK. In vitro antioxidant activities of the fractions of Coccinia grandis L. Leaf extract. Afr J Tradit Complement Altern Med 2007;5(1):61-73.

Cao G, Sofic E, Prior RL. Antioxidant and pro-oxidative behavior of flavonoids: Structure activity relationships. Free Radic Biol Med 1997;22(5):749-60.

Pan Y, Wang K, Huang S, Wang H, Mu X, He C, et al. Antioxidant activity of microwave-assisted extract of longan (Dimocarpus longum Lour.) Peel. Food Chem 2008;106(3):1264-70.

Hasan MS, Ahmed MI, Mondal S, Uddin SJ, Masud MM, Sadhu SK, et al. Antioxidant, antinociceptive activity and general toxicity study of Dendrophthoe falcata and isolation of quercitin as the major component. Orient Pharm Exp Med 2006;6(4):355-60.

Koleva II, van Beek TA, Linssen JP, de Groot A, Evstatieva LN. Screening of plant extracts for antioxidant activity: A comparative study on three testing methods. Phytochem Anal 2002;13(1):8-17.

Meir S, Kanner J, Akiri B, Hada SP. Determination and involvement of aqueous reducing compounds in oxidative defense system of various senescing leaf. J Agric Food Chem 1995;43(7):1813-9.

Dalimunthe A, Achmad S, Satria D. Phenolic, flavonoid content and antioxidant activities of ethylacetate extract of Litsea cubeba (Lour.) Pers. Barks. Pharm Chem 2016;8(19):466-8.

Shah A, Singh T, Rekha V. In vitro antioxidant properties and total phenolic and flavonoid contents of Rumex vesicaius L. Int J Pharm Pharm Sci 2015;7(7):81-4.

Satria D, Silalahi J, Haro G, Ilyas S, Hasibuan PA. Antioxidant and antiproliferative activities of an ethylacetate fraction of Picria fel-terrae Lour. Herbs. Asian Pac J Cancer Prev 2017;18(2):399-403.




About this article

Title

TOTAL PHENOLIC AND FLAVONOID CONTENTS AND ANTIOXIDANT ACTIVITY OF ETHANOL FRACTION OF PICRIA FEL-TERRAE (LOUR.) HERBS

Keywords

Phenolic, Flavonoid, Antioxidant, P. fel-terrae Lour., Ethanol, Herbs.

DOI

10.22159/ajpcr.2017.v10i7.17982

Date

01-07-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 7 July 2017 Page: 243-245

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

41 Views | 52 Downloads

Authors & Affiliations

Panal Sitorus
Department of Pharmaceutical Biology, Faculty of Pharmacy, University of Sumatera Utara, Indonesia.
Indonesia

Poppy Anjelisazaitun Hasibuan
Department of Pharmacology, Faculty of Pharmacy, University of Sumatera Utara, Indonesia.
Indonesia

Denny Satria
Department of Pharmaceutical Biology, Faculty of Pharmacy, University of Sumatera Utara, Indonesia.
Indonesia


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