THE EFFECT OF TWEEN 80, PALM KERNEL OIL, AND ITS CONVERSION PRODUCT ON IN VITRO PENETRATION ENHANCEMENT OF INDOMETHACIN THROUGH RABBIT SKIN
Keywords:Indomethacin, Penetration, Tween 80, Palm kernel oil, Palm kernel oil conversion products
Objective: Objective of the study was to evaluate the effect of Tween 80, palm kernel oil (PKO), and its conversion products (hydrolyzed and transesterificated products) on the in vitro rabbit skin penetration of indomethacin from hydrocarbon ointment base.
Materials and Methods: In vitro penetration of indomethacin through hairless rabbit skin from hydrocarbon ointment base (white petrolatum) was evaluated using a diffusion cell. The effects of Tween 80, PKO, and its conversion products at various concentrations were evaluated.
Results: The higher of the concentration of Tween 80 until 5%, the higher of the indomethacin penetration. However, at the concentration above 5%, the penetration was decreased with the increasing concentration of Tween 80 used. The amount of indomethacin penetrated increased with the increasing of the concentration of PKO and its conversion products used. Hydrolyzed product showed the higher indomethacin penetration enhancing effect than PKO. Transesterificated product showed the highest penetration enhancing effect. However, the combination of Tween 80 with transesterificated product showed lower penetration enhancing effect compared to transesterificated product only.
Conclusion: Tween 80 at low concentrations, PKO, and its conversion products could be used to enhance indomethacin penetration.
Insel PA. Analgetics-antipyretics and anti-inflamatory agents: Drugs employed in the treatment of rheumatoid arthritis and gout. In: Gilman AG, Rall TW, Nies AS, Taylor P, editors. The Pharmacological Basic of Therapeutics. 8th ed. New York: Pergamon Press; 1990. p. 639-81.
Trommer H, Neubert RH. Overcoming the stratum corneum: The modulation of skin penetration. A review. Skin Pharmacol Physiol 2006;19(2):106-21.
Moffat AC, Osselton MD, Widdop B. Clarkeâ€™s Analysis of Drugs and Poisons. London: Pharmaceutical Press; 2005. p. 366-9.
Barry BW. Dermatological Formulation: Percutaneous Absorption. New York: Marcel Dekker, Inc.; 1983. p. 8-13.
Williams AC, Barry BW. Penetration enhancers. Adv Drug Deliv Rev 2004;56:603-18.
Jona JA, Dittert LW, Crooks PA, Milosovich SM, Hussain AA. Design of novel prodrugs for the enhancement of the transdermal penetration of indomethacin. Int J Pharm 1995;123(1):127-36.
Prakash PR, Rao NG, Soujanya C. Formulation, evaluation and anti-inflamatory activity of topical etoricoxib gel. Asian J Pharm Clin Res 2010;3(2):126-9.
Alamu OJ, Waheed MA, Jekayinfa SO. Effect of ethanol palm kernel oil ratio on alkali-catalyzed biodiesel yield. Fuel 2008;87(8-9):1529-33.
Shinde M, Gharge V, Pimple S, Gurjar M, Shah M. Effect of penetration enhancer on the in vitro ex vivo permeation of diclofenac gel. Asian J Pharm Clin Res 2014;7(5):255-9.
Akhtar N, Rehman MU, Khan HM, Rasool F, Saeed T, Murtaza G. Penetration enhancing effect of polysorbate 20 and 80 on the in vitro percutaneous absorption of L-ascorbic acid. Trop J Pharm Res 2011;10(3):281-8.
Agus D, Anayanti A, Hakim B. Study of the effects of tween 80 and palm kernel oil on in vitro ascorbic acid penetration through rabbit skin. Int J PharmTech Res 2013;5(3):965-72.
Patel KN, Patel HK, Patel VA. Formulation and characterization of drug in adhesive transdermal patches of diclofenac acid. Int J Pharm Pharm Sci 2012;4(1):26-34.
Denge MS, Walde SR, Ittadwar AM. Development and characterization of transdermal patches of ondansetron hydrochloride. Int J Pharm Pharm Sci 2012;4 Suppl 5:293-8.
Patrick JS, Yashveer S. Martinâ€™s Physical Pharmacy and Pharmaceutical Sciences. 6th ed. Baltimore, MD: Lippincott Williams & Wilkins; 2011. p. 389-90.
Golden GM, McKie JE, Potts RO. Role of stratum corneum lipid fluidity in transdermal drug flux. J Pharm Sci 1987;76(1):25-8.
Komata Y, Kaneko A, Fujie T. Effect of fatty acid on the accumulation of thiamine disulfide in rat skin. Biol Pharm Bull 1994;17(5):705-8.
David F, Paul C, Jorge H. Simple alkyl esters as skin permeation enhancers. J Control Release 1989;9(1):33-41.
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