THE COMPARISON OF THE EFFECT BETWEEN ALGINATE-BASED RAFT-FORMING LIQUID AND ALGINATE LIQUID ON GASTROESOPHAGEAL REFLUX DISEASE AND GASTRIC ULCER IN RATS


Hakim Bangun, Anayanti Arianto, Ririn Astya, Gontar A Siregar

Abstract


Objective: The objective of the study was to compare the effect between alginate (Alg)-based raft-forming and Alg liquid on healing gastroesophageal reflux disease (GERD) and gastric ulcer in rats.

Methods: Each of the 18 fasted rats was given 1 ml acidified pepsin. Then, rats were divided into three groups. Each group consisted of six rats. Group 1 (negative control) was orally given 1 ml distilled water, Group 2 was given 1 ml Alg-based raft-forming liquid, and Group 3 was given 1 ml Alg liquid. Then, the abdomen of rats was incised under anesthesia with ketamine, and then both their pylorus and the forestomach were ligated to form gastric reflux. After 4 hrs, all rats were killed with chloroform and their esophagus and stomach were examined macroscopically and microscopically (histopathology).

Results: On macroscopic observation, all of the Group 1 rats (negative control) showed esophageal lesions and gastric lesions. Four rats of Group 2 (given Alg-based raft-forming) showed no esophageal lesion and two more rats showed a slight lesion, but all of the tested rats showed gastric lesions. All of the rats of the Group 3 (given Alg liquid) showed esophageal lesions, but no gastric lesion on four rats and slight lesion on two rats. Microscopic observations showed that all of the Group 1 rats (negative control) showed esophageal erosion and gastric mucosa lesions. Rats of the Group 2 (given Alg-based raft-forming) showed almost no esophageal erosion, but all of them showed erosion of gastric mucosa. Rats of the Group 3 (given Alg liquid) showed esophageal erosion in all tested rats, but almost no gastric mucosa lesion.

Conclusion: Alg-base raft-forming liquid is more effective in healing of GERD than Alg liquid. However, Alg-liquid is more effective in healing of gastric ulcer than Alg-based raft-forming liquid.


Keywords


Alginate-Based, Raft-Forming, Alginate Liquid, GERD, Gastric Ulcer, Healing, Comparison

| PDF |

References


APhA (American Pharmaceutical Association). Handbook of Nonprescription Drugs. 8th ed. Washington, DC: American Pharmaceutical Association; 1986. p. 22.

McPhee JS, Ganong FW. Pathophysiology of Disease: An Introduction to Clinical Medicine. 5th ed. Ch. 13. USA: The McGraw-Hill; 2006.

Katzung GB, Trevor JA. Basic and Clinical Pharmacology. 13th ed. New York: McGraw-Hill; 2015. p. 1429.

Nasa P, Mahant S, Sharma D. Floating system: A novel approach towards gastroretentive drug delivery system. Int J Pharm Pharm Sci 2010;2(3):2-7.

Mandel KG, Daggy BP, Brodie DA, Jacoby HI. Review article: Alginate-raft formulations in the treatment of heartburn and acid reflux. Aliment Pharmacol Ther 2000;14(6):669-90.

Aryana, Sinurat D, Revina I, Bangun H. Formulation and in vitro evaluation of alginate based metronidazole periodontal gel. Asian J Pharm Clin Res 2014;7(1):223-7.

Adlin N, Bangun H, Karsono. Preparation and evaluation of floating-mucoadhesive alginate beads as gastroretentive drug delivery system of antacids. Int J PharmTech Res 2016;9(5):212-22.

Mariadi, Bangun H, Karsono. Formulation and in vitro evaluation of gastroretentive drug delivery system of antacids using alginate-chitosan films. Int J PharmTech Res 2015;8(9):1-12.

Kulkarni P, Keshavayya J. Chitosan-sodium alginate biodegradable interpenetrating polymer network (IPN) for delivery of ofloxacin hydrochloride. Int Pharm Pharm Sci 2010;2(2):77-82.

Arianto A. Antiulcer effect of gastroretentive spherical matrices of alginate-chitosan containing ranitidine HCL. Int J PharmTech Res 2016;9(5):342-52.

Arianto A, Bangun H, Yohana A, Silalahi J. Floating gastroretentive of amoxicillin using hard alginate capsules and antibacterial activities. Asian J Pharm Clin Res 2017;10(5):413-9.

Hasan JM, Kamal AB. Formulation and evaluation of ranitidine hydrochloride as floating in situ gel. Int J Pharm Pharm Sci 2014;6 Suppl 2:401-15.

Morsi N, Ghorab D, Refai H, Teba H. Preparation and evaluation of alginate/chitosan nanodispersion for ocular delivery. Int J Pharm Pharm Sci 2015;7(7):234-40.

Sun J, Huaping T. Alginate-based biomaterial for regenerative medicine applications. J Mater 2013;6:1285-309.

Arianto A, Bangun H. Healing effect of alginate liquid against HCL-induced gastric mucosal lesion in rats. Int J PharmTech Res 2016;9(3):287-96.

Smidsrød O, Skjåk-Braek G. Alginate as immobilization matrix for cells. Trends Biotechnol 1990;8(3):71-8.

Withington R. Pharmaceutical Compositions for Use in the Suppression of Gastric Reflux. 1979; United States Patent. p. 1-5.

Takeuchi K, Nagahama K. Animal model of acid-reflux esophagitis: Pathogenic roles of acid/pepsin, prostaglandins, and amino acids. Biomed Res Int 2014;2014:532594.

Price SA, Wilson LM. Pathophysiology: Clinical Concepts of Disease Processes. 3rd ed. New York: McGraw-Hill Book Company; 1986. p. 242-58.

Arianto A, Bangun H, Harahap U, Ilyas S. The comparison of swelling, mucoadhesive, and release of ranitidine from spherical matrices of alginate, chitosan, and calcium alginate-chitosan. Int J PharmTech Res 2014;6(7):2054-63.

Daigo K, Wada Y, Yamada C, Yamaji M, Okuda S, Okada M, et al. Pharmacological studies of sodium alginate. I. Protective effects of sodium alginate on mucous membranes of uppergastrointestinaal tract. Yakugaku Zasshi 1981;101(5):452-7.




About this article

Title

THE COMPARISON OF THE EFFECT BETWEEN ALGINATE-BASED RAFT-FORMING LIQUID AND ALGINATE LIQUID ON GASTROESOPHAGEAL REFLUX DISEASE AND GASTRIC ULCER IN RATS

Topics

Pharmacy

Keywords

Alginate-Based, Raft-Forming, Alginate Liquid, GERD, Gastric Ulcer, Healing, Comparison

DOI

10.22159/ajpcr.2017.v10i12.21096

Date

01-12-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 12 December 2017 Page: 105-110

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

15 Views | 32 Downloads

Authors & Affiliations

Hakim Bangun
Department of Pharmaceutical Technology, Faculty of Pharmacy, Nanomedicine Center, University of Sumatera Utara, Jl. Tri Dharma No. 5, Kampus USU, Medan, Indonesia.
Indonesia

Anayanti Arianto
Department of Pharmaceutical Technology, Faculty of Pharmacy, Nanomedicine Center, University of Sumatera Utara, Jl. Tri Dharma No. 5, Kampus USU, Medan, Indonesia.
Indonesia

Ririn Astya
Department of Pharmaceutical Technology, Faculty of Pharmacy, Nanomedicine Center, University of Sumatera Utara, Jl. Tri Dharma No. 5, Kampus USU, Medan, Indonesia.
Indonesia

Gontar A Siregar
Department of Internal Medicine, Faculty of Medicine, University of Sumatera Utara, Jl Dr. T. Mansyur No. 5, Kampus USU, Medan, Indonesia.
Indonesia


Article Tools


Email this article (Login required)
Email the author (Login required)

Refbacks

  • There are currently no refbacks.