AMELIORATING THE METABOLIC DISORDER IN STREPTOZOTOCIN-INDUCED DIABETIC RATS BY HAEMATOCOCCUS PLUVIALIS EXTRACT


Farouk Kamel Elbaz, Hanan F Aly

Abstract


Objective: The purpose of the current study is to evaluate the antidiabetic influence of Haematococcus pluvialis (H. pluvialis) ethanolic extract on streptozotocin (STZ)-induced rats.

Methods: This influence was assessed by measuring; asymmetric dimethylarginine (ADMA), von willebrand factor (vWf), 15-lipoxygenase (15-LOX), 5-lipoxygenase (5-LOX), epidermal growth factor (EGF) and lipoxin-A4 (LXA4). The histopathological examinations of cardiac and renal tissues were also performed.

Results: The presented  results showed an elevation in the levels of ADMA, vWf, 15-LOXand 5-LOX with percentage increase  430.30, 77.54, 61.05 and 81.09 %, respectively in diabetic rats. However, the EGF and LXA4 levels were decreased significantly (44.17 and51.94%, respectively) compared to normal control rats. On the other hand, treatment of diabetic rats with H. pluvialis improved ADMA, vWf, 15-LOX, 5LOX, EGF and LXA4 levels with amelioration percentages; 269.69, 54.77, 55.78, 72.68, 53.39 and 56.58%, respectively. Additionally, the histopathological examination of diabetic cardiac tissue revealed congestion of myocardial blood vessel, vacuolation of the sarcoplasm of cardiac myocytes and focal necrosis of myocytes associated with inflammatory cells infiltration. Beside, vacuolation of epithelial lining of renal tubules and endothelial lining glomerular tuft as well as cystic dilatation of renal tubules were detected. However, cardiac tissue  and renal tubules of diabetic rats treated with H. pluvialis ethanolic extract (150 mg/kg b.wt.) showed normal cardiac myocytes, vacuolation of epithelial lining renal tubules and endothelial lining glomerular tuft, congestion of glomerular tuft and intertubular renal blood vessels.

Conclusion: These results confirmed the improving role of H. pluvialis in controlling type 2diabetes.

 


Keywords


Haematococcus pluvialis, Streptozotocin, Type 2 diabetes, Asymmetric dimethylarginine, Von Willebrand factor, Lipoxygenases, Histopathological examination.

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References


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About this article

Title

AMELIORATING THE METABOLIC DISORDER IN STREPTOZOTOCIN-INDUCED DIABETIC RATS BY HAEMATOCOCCUS PLUVIALIS EXTRACT

Keywords

Haematococcus pluvialis, Streptozotocin, Type 2 diabetes, Asymmetric dimethylarginine, Von Willebrand factor, Lipoxygenases, Histopathological examination.

DOI

10.22159/ajpcr.2017.v10i6.17785

Date

01-06-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 6 June 2017 Page: 72-77

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

102 Views | 135 Downloads

Authors & Affiliations

Farouk Kamel Elbaz
Department of Plant Biochemistry, National Research Centre, Giza, Egypt.
Egypt

Hanan F Aly
Department of Therapeutic Chemistry, National Research Centre, Giza, Egypt
Egypt


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