BIOINFORMATICS: INFLAMMATORY CYTOKINES AND ATTENUATION OF DIABETES HYPERCHOLESTEROLEMIA-INDUCED RENAL INJURY USING MORNING GLORY AND NECKLACE POD EXTRACTS

  • Hanan F Aly Department of Therapeutic Chemistry, National Research Centre, Dokki, Giza, Egypt http://orcid.org/0000-0001-9638-8481
  • Howaida I Abd-alla
  • Howaida I Abd-alla Department of Chemistry of Natural Compounds, National Research Centre, Dokki, Giza, Egypt
  • Sanaa A Ali
  • Sanaa A Ali Department of Therapeutic Chemistry, National Research Centre, Dokki, Giza, Egypt
  • Reda Aba Alez
  • Reda Aba Alez Department of Information Systems and Computer Engineering, Faculty of Engineering, Al-Azhar University, Cairo, Egypt
  • Mohammed T Abu-krisha
  • Mohammed T Abu-krisha Department of Information Systems and Computer Engineering, Faculty of Engineering, Al-Azhar University, Cairo, Egypt
  • Mohamed M Mamdouh
  • Mohamed M Mamdouh Department of Information Systems and Computer Engineering, Faculty of Engineering, Al-Azhar University, Cairo, Egypt

Abstract

 

 Objective: The present research in bioinformatics focuses on pharmacological effects of morning glory and necklace pod ethanolic extracts (MGE and NPE) on some biochemical parameters in high fat diet-induced hypercholesterolemia and streptozotocin-induced hyperglycemia in rats.

Methods: Compared to atorvastatin; an anti-hypercholesterolemic (HC) and glibenclamide; an antidiabetic drug. Endothelium activation markers of soluble vascular cell adhesion molecule (VCAM)-1 and intercellular adhesion molecule (ICAM)-1 were determined using enzyme-linked immunosorbent assay. Creatinine, urea, and inflammatory biomarkers; C-reactive protein (CRP) and pro-inflammatory cytokines including tumor necrosis factor alpha (TNF-α) and interleukin (IL)-10 levels were also measured in serum of different therapeutic groups.

Results: Significant decrease in ICAM-1 level with MGE and NPE supplemented to normal rats as compared to untreated control with percentages decrease 17.80 and 12.00% was observed. Insignificant change was detected in VCAM-1 level. Profound amelioration in CRP, total urea and creatinine levels by NPE treatment. Creatinine, urea, CRP, and TNF-α level were significantly increased in hyperglycemic (HG)-HC rats. However, IL-10 level showed a significant decrease. Meanwhile, histopathological investigation of the kidney and heart was carried out. Image recognition system for kidney and heart images was developed to diagnose their diseases. Tested extract attenuated creatinine, urea, CRP, and TNF-α level. Hyperglycemia and hypercholesterolemia linked kidney disorders were relieved.

Conclusion: In vivo oral administration with each extract declared suppression of cytokines mediated inflammation, vascular function leading to infiltration reduction of renal macrophage together with lowering in kidney indices and ameliorate renal tissues architectures in HG-HC rats.

Keywords: Morning glory, Necklace pod, Inflammatory, Cell adhesion, Kidney function, Statistic, Image recognition.

Author Biography

Hanan F Aly, Department of Therapeutic Chemistry, National Research Centre, Dokki, Giza, Egypt
Chemistry of Natural Compounds

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F Aly, H., H. I. Abd-alla, H. I. Abd-alla, S. A. Ali, S. A. Ali, R. A. Alez, R. A. Alez, M. T. Abu-krisha, M. T. Abu-krisha, M. M. Mamdouh, and M. M. Mamdouh. “BIOINFORMATICS: INFLAMMATORY CYTOKINES AND ATTENUATION OF DIABETES HYPERCHOLESTEROLEMIA-INDUCED RENAL INJURY USING MORNING GLORY AND NECKLACE POD EXTRACTS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, no. 11, Nov. 2017, pp. 347-55, doi:10.22159/ajpcr.2017.v10i11.20119.
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