• Mounir M El-safty Department of Evaluation of Inactivated Viral Poultry Vaccines, Central Laboratory for Evaluation of Veterinary Biologics, Abbassia 13181, Cairo, Egypt.
  • Hala Mahmoud Department of Evaluation of Inactivated Viral Poultry Vaccines, Central Laboratory for Evaluation of Veterinary Biologics, Abbassia 13181, Cairo, Egypt.
  • Eman Sa Zaki Department of Aerobic Bacterial Vaccines, Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt.
  • Howaida I Abd-alla Department of Chemistry of Natural Compounds, Pharmaceutical Industries Division, National Research Centre, Dokki-Giza 12622,



 Objective: Salmonella enteritidis ghosts (SEGs) is a non-living empty bacterial cell envelopes which were generated using a different concentration of sodium hydroxide (NaOH) 6.4 mg/mL and evaluated as a vaccine candidate in specific pathogen-free (SPF) chicken. SEGs have been produced by chemical-mediated lysis and evaluated the potential efficacy of chemically induced SEG vaccine and its ability to induce protective immune responses against virulent S. enteritidis challenge in SPF chickens.

Methods: SPF chickens were divided into three groups: Group A (non-vaccinated control), Group B (vaccinated with prepared vaccine), and Group C (vaccinated with commercial vaccine).

Results: Vaccination of SPF chicken with SEGs induced higher immune responses before and after virulent challenge. SPF chicken vaccinated with SEGs showed increasing in serum enzyme-linked immunosorbent assay (ELISA) antibodies. During the vaccination period, Groups B and C showed higher serum antibody titer compared to Group A. The minimal inhibitory concentration (MIC) of NaOH was capable of inducing non-living SEGs, and it has successfully generated non-living SEGs by MIC of NaOH.

Conclusion: It is a one-step process which means easy manufacturing and low production cost compared to protein E-mediated lysis method. Chemically induced SEG vaccine is a highly effective method for inducing protective immunity. This study strongly suggests that SEGs will be a permissive vaccine, as the method of inhibition of S. enteritidis was safe and cheaper than other methods, and it gave a good protection.

Keywords: Salmonella enteritidis ghost, Specific pathogen-free, Vaccine, Enzyme-linked immunosorbent assay, Sodium dodecyl sulfate polyacrylamide gel electrophoresis.

Author Biography

Mounir M El-safty, Department of Evaluation of Inactivated Viral Poultry Vaccines, Central Laboratory for Evaluation of Veterinary Biologics, Abbassia 13181, Cairo, Egypt.
Biological study


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How to Cite
El-safty, M. M., H. Mahmoud, E. S. Zaki, and H. I. Abd-alla. “PREPARATION AND EVALUATION OF CHEMICALLY INACTIVATED SALMONELLA ENTERITIDIS VACCINE IN CHICKENS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, no. 11, Nov. 2017, pp. 341-6, doi:10.22159/ajpcr.2017.v10i11.20144.
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