IMPACT OF FERMENTING AGENTS ON MARKETED PATENT PROPRIETARY FORMULATION ASHWAGANDHA KALPA
Â Objective: The present work is aimed to formulate Ashwagandha kalpa and evaluate the impact of fermenting agent.
Methods: A. kalpa is a marketed patent proprietary Ayurvedic product prepared by adopting the Ayurvedic principles of madya sandhana and it belongs to the class of formulations known as Sandhana kalpana. It is given to the patients suffering from hypertension, insomnia, paralysis, loss of concentration, etc. In the preparation of madya sandhana, the sandhana dravya (fermenting agents) play a key role. The present study was taken up to see the effect of three different sandhana dravya (fermenting agents), i.e., Dhataki pushpa (DAK), Madhuka (MAK), and yeast (YAK) on the formulation characterization of A. kalpa.
Results: The prepared samples were tested for their physicochemical parameters, i.e., total solid, pH, specific gravity, sugar content, alcohol content, refractive index, phytochemical screening, quantitative assay for secondary metabolites, and high-performance thin-layer chromatography (HPTLC) fingerprinting. The findings of phytochemical evaluation, quantitative assay, and HPTLC fingerprint show marked variation among three different prepared samples of A. kalpa. The preliminary phytochemical estimation for the detection of secondary metabolites was done. The study revealed the presence of highest percentage of alkaloids in YAK, tannins in DAK and saponins in YAK. Total solid content was found to the highest in MAK. Physicochemical parameters such as pH, total solid content, refractive index, alcohol content, and specific gravity also showed marked variation. Alcohol content for the DAK and YAK was found same.
Conclusion: sandhana dravya (fermenting agent) causes variations in different physicochemical and phytochemical parameters in the formulation and development of A. kalpa.
2. Chaudhary AK, Kanjiv L. Bhaishjya Ratnawali of Govinda Dasji. 1st ed., Vol. 3. Varanasi: Chaukhambha Sanskrit Sansthana; 2006. p. 100-54, 365-70.
3. Muralidhar R, Chaudhary A, Ravishankar B, Dey S, Prajapati PK. A comparative pharmaceutico-pharmacoclinical study of different samples of Shirisharishta and its shwashara effect. AYU 2004;7:45-9.
4. Lakhani R, Chaudhary AK. A comparative pharmaceutico-clinical study on Arka Kalpana and Arishta Kalpana W.S.R. to Jirakadyarka and Jirakadyarishta on Grahani. Sachitra Ayurveda 2004;8:145-8.
5. Bajaj V, Chaudhary AK. Validation process in Sandhan Kalpanaâ€ Sachitra. Ayurveda 2002;5:846-8.
6. Gandhi P, Chaudhary AK, Ravishankar B, Dey S, Prajapati PK. A Comparative Study of Different Formulations of Vasa (Avaleha, Sneha, Sandhan) WSR to its Swasahar Effect, MD (AY) Dissertation of IPGTRA. Jamnagar: Gujarat Ayurveda University; 2005.
7. Shastri MV. Vaidya Yoga Ratnavali. Madras: IMPCOPS; 1968. p. 6-10.
8. Vohra A, Satyanarayana T. Phytase production by the yeast Pichia anomala. Biotechnol Lett 2001;23:551-4.
9. Vohra A, Satyanarayana T. A cost-effective cane molasses medium for enhanced cell-bound phytase production by Pichia anomala. J Appl Microbiol 2004;97(3):471-6.
10. Hiremath SG, Joshi D. Role of different containers and methods on alcoholic preparations with reference to kutajarista. Anc Sci Life 1991;10(4):256-63.
11. William H. Official Method of Analysis. Washington: Association of Official Agricultural Chemists; 1960. p. 185.
12. Gupta AK, Tandan N, Sharma M. Quality Standards of Indian Medicinal Plants. Vol. 6. New Delhi: Medicinal Plants Unit, Indian Council of Medical Research; 2008. p. 290.
13. Anonymous. The Ayurvedic Pharmacopoeia of India. Part-1. Vol. 1. Ministry of Health and Family Welfare, Government of India, Department of ISM & H; 1999. p. 140-3.
14. Anonymous. Protocol for Testing of Ayurvedic, Siddha and Unani Medicines. Pharmacopoeial Laboratory for Indian Medicine, AYUSH. Ghaziabad: Ministry of Health and Family Welfare, Government of India; 2011.
15. Ministry of Health and Family Welfare, Government of India. Pharmacopoeial Standards for Ayurvedic Formulations CCRAS. New Delhi: Ministry of Health and Family Welfare, Government of India; 1987.
16. Shankar R. Text Book of Pharmaceutical Analysis. Otacamund: Rx Publication; 2001.
17. Baxi AJ, Shukla VJ, Bhatt UB. Methods of Qualitative Testing of some Ayurvedic Formulation. Jamnagar: Gujarat Ayurved University; 2001.
18. Mendham J, Denney RC, Barnes JD, Thomas MJ. Vogelâ€™s Text Book of Quantitative Chemical Analysis. 6th ed. Essex: Pearson Education Asia; 2002.
19. Anonymous. Quality Standard of Indian Medicine Plants. Vol. 2. New Delhi: Indian Council of Medical Research; 2005.
20. Kasture AV, Wadodkar SG, Mahadik KR, More HN. Industrial methods. Pharmaceutical Analysis. Vol. 2. Pune: Nirali Prakashan; 2008.
21. Anonymous. Planner chromatography. Modern Thin Layer Chromatography. Switzerland: CAMAG Muttenz; 1999. p. 2-16.
22. Chahal MK, Ydav PK, Bumbrah GS, Sharma RM. Thin layer chromatographic analysis of some common over the counter (OTC) cough-cold preparations. Egypt J Forensic Sci 2016;6:351-60.
23. Stahl E. Thin-Layer-Chromatography: A Laboratory Hand Book. Translated by Ashworth MR. 2nd ed. Berlin, Heidelberg, New York: Spring-Verag; 1969.
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