COMAPARTIVE STUDY OF ANTIBACTERIAL AND ANTI-PROLIFERATIVE POTENTIAL OF GREEN TEA FROM DIFFERENT GEOGRAPHICAL LOCATIONS IN INDIA
Objective: The aim of this study was to investigate the in vitro synergistic antibacterial effect of different solvent extracts of green tea (GT) with
ampicillin and interpreting the anti-proliferative activity of purified GT catechins.
Methods: The methanolic, acetone, and aqueous extracts of GT leaves consumed in Assam (AT), Himachal Pradesh (HP) and
Uttarakhand regions (IP and PN) of North India were used in the study. The synergistic antibacterial properties of GT with ampicillin were determined
against Pseudomonas aeruginosa microbial type culture collection (MTCC) 4306, Staphylococcus aureus MTCC 6908 and Escherichia coli MTCC 1698
using agar well diffusion assay. The purified catechins from different GT leaves were assessed for their anti-proliferative potential on C6 glioma cell
lines using 3-(4, 5-dimethyl thiazol-2-yl)-5-diphenyltetrazolium bromide (MTT) assay.
Results: The aqueous extract of different GT alone showed no inhibition against all bacterial strains used, whereas methanolic and acetone extracts
revealed significant antibacterial activity (p<0.05). Combination of methanolic extracts of AT with ampicillin showed highest synergistic inhibition
of 39Â±1.414 mm and 36Â±0.070 mm against S. aureus and P. aeruginosa respectively with an increase of 290Â±0.070% and 260Â±0.070% inhibition
with respect to their individual effect. MTT assay depicted higher growth inhibition of 95.75Â±0.862-89.47Â±0.14% on treated glial cells. However, a
significant difference in the inhibitory concentration (IC90) of the purified catechins from different GT leaves (p<0.05) was observed.
Conclusion: GT leaves have potential anti-proliferative activity and synergistic antibacterial activity. Further in vivo and clinical studies are required
to confirm its therapeutic efficacy.
Keywords: Green tea catechins, Antibacterial, Anti-proliferative, MTT assay, Antibiotic resistance.
2. Lamichhane B, Thakur C, Jain SK. Antibiotic resistance patterns of Gram-negative isolates in a tertiary care hospital of Nepal. Asian J Pharm Clin Res 2014;7:30-3.
3. Pradeepa S, Subramanian S, Kaviyarasan V. Evaluation of antimicrobial activity of Pithecellobium dulce pod pulp extract. Asian J Pharm Clin Res 2014;7:32-7.
4. Torella JP, Chait R, Kishony R. Optimal drug synergy in antimicrobial treatments. PLoS Comput Biol 2010;6:1000796.
5. zur Wiesch PA, Kouyos R, EngelstÃ¤dter J, Regoes RR, Bonhoeffer S. Population biological principles of drug-resistance evolution in infectious diseases. Lancet Infect Dis 2011;11:236-47.
6. Anand J, Upadhyaya B, Rawat P, Rai N. Biochemical characterization and pharmacognostic evaluation of purified catechins in green tea (Camellia sinensis) cultivars of India. 3 Biotech 2014; doi: 10.1007/s13205-014-0230-0:S13205. (In Press)
7. Radji M, Agustama RA, Elya B, Tjampakasari CR. Antimicrobial activity of green tea extract against isolates of methicillin resistant Staphylococcus aureus and multi-drug resistant Pseudomonas aeruginosa. Asian Pac J Trop Biomed 2013;3:663-7.
8. Chan EW, Soh EY, Tie PP, Law YP. Antioxidant and antibacterial properties of green, black, and herbal teas of Camellia sinensis. Pharmacogn Res 2011;3(4):266-72.
9. Koh GY, Chou G, Liu Z. Purification of a water extract of Chinese sweet tea plant (Rubus suavissimus S. Lee) by alcohol precipitation. J Agric Food Chem 2009;57(11):5000-6.
10. Archana S, Jayanthi A. Comparative analysis of antimicrobial activity of leaf extract from fresh green tea, commercial green tea and black tea on pathogens. J Appl Pharm Sci 2011;1:149-52.
11. Ofokansi KC, Attama AA, Uzor PF, Ovri MO. Evaluation of the combined antimicrobial activity of the leaf extract of phyllantus muellerianus with ciprofloxacin. J Pharm Technol Drug Res 2013;2:16.
12. Geetha B, Santhy KS. Anti-proliferative activity of green tea extract in human cervical cancer cells (HeLa). Int J Curr Microbiol Appl Sci 2013;2:341-6.
13. Hill JA, Ammar R, Torti D, Nislow C, Cowen LE. Genetic and genomic architecture of the evolution of resistance to antifungal drug combinations. PLoS Genet 2013;9(4):1003390.
14. Tsuchiya H, Sato M, Miyazaki T, Fujiwara S, Tanigaki S, Ohyama M, et al. Comparative study on the antibacterial activities of phytochemical flavanones against methanolic resistance Staphylococcus aureus. J Ethanopharmacol 1996;50:27-34.
15. Julieth C, Veronica T, Camilo D, Elena S, Ana MA, et al. In vitro antifungal activity and cytotoxicity effect of essential oils and extracts of medicinal and aromatic plants against Candida krusei and Aspergillus fumigatus. Rev Bras Farmacogn 2010;20:734-1.
16. Anand A, Rai N, Kumar N, Gautam P. Green tea: A magical herb with miraculous outcomes. Int Res J Pharm 2012;3:139-47.
17. Haghjoo B, Lee LH, Habiba U, Tahir H, Olabi M, Chu T. The synergistic effects of green tea polyphenols and antibiotics against potential pathogens. Adv Biosci Biotechnol 2013;4:956-67.
18. Ledy GB, Catalina LR, Azacena OC, Catalina RM. Free radical scavenging and cytotoxic activity of five commercial standardized extracts (red wine, green tea, pine bark, polygonum and pomegranate). Afr J Biotechnol 2012;11:16725-30.
19. Hsu S, Bollag WB, Lewis J, Huang Q, Singh B, Sharawy M, Yamamoto T, Schuster G. Green tea polyphenols induce differentiation and proliferation in epidermal keratinocytes. J Pharmacol Exp Ther 2003; 306(1):29-34.
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