GC-MS ANALYSIS OF METHANOLIC EXTRACT OF LEAVES OF RHODODENDRON ARBOREUM

  • Sakshi Painuli Department of Biotechnology, Graphic Era University. Dehradun, Uttarakhand
  • Nishant Rai Department of Biotechnology, Graphic Era University. Dehradun, Uttarakhand
  • Navin Kumar Department of Biotechnology, Graphic Era University. Dehradun, Uttarakhand

Abstract

ABSTRACT

Objective: The objective of the present study is to determine the possible phytoconstituents identified by gas chromatography and mass spectrometry
(GC-MS) analysis of a methanolic leaf extract of Rhododendron arboreum (RAM).
Method: The extraction of RAM was done by accelerated solvent extraction system at room temperature and high pressure using methanol as a
solvent. GC-MS analysis of lyophilized methanolic leaf extract of plant samples was carried out by GCMS-QP2010 plus (Shimadzu, Kyoto, Japan).
Results: In RAM, 34 phytochemicals were identified among which 22-stigmasten-3-one showed the highest area (14.59%) and benzyl acetate showed
the lowest area (0.15%). The major compounds identified were 22-stigmasten-3-one, 1,1,6-trimethyl-3-methylene-2-(3,6,10,13,14-pentamethyl3-ethenyl-pentadec-4-enye)cyclohexane,
alpha-amyrin,
beta-amyrin,
linoleyl
alcohol,
linoleic,
beta-citronellol,
tetradecane,
9,12-octadecadienoic
acid,
methyl
ester,
dibutyl
phthalate,
L-ascorbic
acid 2, 6-dihexadecanoate,
dodecane, and heptadecane.
Conclusion: GC-MS analysis revealed the presence of hydrocarbon alkane, ester, terpenes, flavonoids, organic compounds, steroids, and fatty acids in
RAM. These active phytoconstituents contribute to the medicinal efficacy of the plant and the plant can be used for the sourcing of these compounds.
Keywords: Rhododendron arboreum, Gas chromatography-mass spectrometry, Phytoconstituents.

Author Biography

Navin Kumar, Department of Biotechnology, Graphic Era University. Dehradun, Uttarakhand
Assistant professor

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How to Cite
Painuli, S., N. Rai, and N. Kumar. “GC-MS ANALYSIS OF METHANOLIC EXTRACT OF LEAVES OF RHODODENDRON ARBOREUM”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 9, no. 1, Jan. 2016, pp. 101-4, https://innovareacademics.in/journals/index.php/ajpcr/article/view/8722.
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Original Article(s)