GAS CHROMATOGRAPHY-MASS SPECTROSCOPY ANALYSIS OF PHYTOCHEMICAL CONSTITUENTS OF METHANOLIC EXTRACT OF NEEDLES OF PINUS WALLICHIANA
Objective: To investigate the phytoconstituents of methanolic extracts of a conifer Pinus wallichiana using gas chromatography-mass spectroscopy
(GC-MS), which is famous for its timber quality, resins and oils.
Methods: The present investigation was carried out to determine the possible chemical constituents present in the methanolic extracts in the needles
of P. wallichiana.
Results: The result of the GC-MS confirmed the presence of 38 compounds. Momeinositol (43.27%), 9, 12-octadecanoic acid, 2 (12.52%) pentadecanoic
acid (4.12%) and dibutyl phthalate (3.34%) were the major compound found through the GC-MS analysis.
Conclusion: The presence of various compounds in the methanolic extract of P. wallichiana confirms its relevance not only for pharmaceutical
purpose but for various industrial purposes too.
Keywords: Pinus wallichiana, Gas chromatography-mass spectroscopy (GC-MS), Gas chromatography-mass spectroscopy of plant extract, Gas
chromatography-mass spectroscopy of conifer, Gas chromatography-mass spectroscopy profiling.
Gurib-Fakim A. Medicinal plants: Traditions of yesterday and drugs of
tomorrow. Mol Aspects Med 2006;27(1):1-93.
Dias DA, Urban S, Roessner U. A historical overview of natural
products in drug discovery. Metabolites 2012;2(2):303-36.
Jain S, Khan MA, Din SU, Morad W, Hussain M, Ghani A. Herbal
remedies used for gastrointestinal disorders in Kaghan Valley, NWFP,
Pakistan. Pak J Weed Sci Res 2008;14:169-200. Available from: http://
Saqib AN, Waseem A, Khan AF, Mahmood Q, Khan A, Habib A, et al.
Arsenic bioremediation by low cost materials derived from Blue Pine
(Pinus wallichiana) and Wallnut (Juglans regia). Ecol Eng 2013;51:8894.
Maimoona A, Naeem I, Saddiqe Z, Ali N, Ahmed G, Shah I. Analysis of
total flavonoids and phenolics in different fractions of bark and needle
extracts of Pinus roxburghii and Pinus wallichiana. J Med Plant Res
;5:2724-8. Available from: http://www.academicjournals.org/
Emami SA, Shahani A, Hassanzadeh Khayyat M. Antioxidant activity
of leaves and fruits of cultivated conifers in Iran. Jundishapur J Nat
Pharm Prod 2013;8(3):113-7.
Naeem I, Taskeen A, Mubeen H, Alya M. Characterization of
flavonols present in barks and needles of Pinus wallichiana and
Pinus roxburghii. Asian J Chem 2010;22:41-4. Available from:
Muthulakshmi A, Margret RJ, Mohan VR. GC-MS analysis of bioactive
components of Feronia elephantum Correa (Rutaceae). J Appl Pharm
Sci 2012;2:69-74. Available from: http://www.imsear.li.mahidol.ac.th/
Sermakkani M, Thangapandian V. GC-MS analysis of Cassia italica
leaf methanol extract. Asian J Pharm Clin Res 2012;5(2):90-4.
Available from: http://www.ajpcr.com/Vol5Issue2/840.pdf.
Syed ZH, Maqbool K. GC-MS principle, technique and its application
in food science. Int J Curr Sci 2014;13:116-26. Available from: http://
Mamta, Mehrotra S, Amitabh, Kirar V, Vats P, Nandi SP, et al.
Phytochemical and antimicrobial activities of Himalayan Cordyceps
sinensis (Berk.) Sacc. Indian J Exp Biol 2015;53(1):36-43.
Das S, Vasudeva N, Sharma S. Chemical composition of ethanol extract
of Macrotyloma uniflorum (Lam.) Verdc. using GC-MS spectroscopy.
Org Med Chem Lett 2014;4(1):13.
Jost R. Milk and Dairy Products, Ullmanns Encyclopedia of Industrial
Chemistry. Wiley-VCH Verlag GmbH & Co. KGaA; 2002. Available
Smedman AE, Gustafsson IB, Berglund LG, Vessby BO. Pentadecanoic
acid in serum as a marker for intake of milk fat: relations between intake
of milk fat and metabolic risk factors. Am J Clin Nutr 1999;69(1):22-9.
Yousuf Dar M, Shah WA, Mubashir S, Rather MA. Chromatographic
analysis, anti-proliferative and radical scavenging activity of Pinus
wallichina essential oil growing in high altitude areas of Kashmir,
India. Phytomedicine 2012;19(13):1228-33.
How to Cite
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.