A, Immobilization of amylase producing bacteria (Bacillus subtilis) from soil of Western Himalayan region Solan, Himachal Pradesh, India

  • PhD candidate Shoolini University of Biotechnology and Management Sciences, School of Biotechnology, Solan, Himachal Pradesh, India
  • Assistant Professor Shoolini University
  • Professor Shoolini University of Biotechnology and Management Sciences, School of Biological and Environmental Sciences, Solan, Himachal Pradesh, India
  • Neeraj Kumar Shoolini Institute of Life Sciences and Business Management, Solan, Himachal Pradesh, India
  • Assistant Professor Shoolini Institute of Life Sciences and Business Management, Solan, Himachal Pradesh, India

Abstract

To apply a massive amount of amylase in industries much amount of it should be produced by using bacteria. The paper aimed to immobilize amylase producing bacterial strain on a suitable matrix and characterization of its physicochemical properties so that much amount of amylase could be produced to be applied in different industries. Bacterial colonies were subcultured from samples collected from soil in freshly prepared dishes containing starch agar by dot method using sterile inoculating needles from which five different bacteria belonged to genus Bacillus were isolated and assigned as A1, A2, A3, A4, and A5. It was found that A1 displayed the highest enzyme activity of 17.89 IU/ml with enzyme assay of 0.83mg/ml and the bacterium was identified to be Bacillus subtilus. A5 displayed 10.13 IU/ml with protein contents of 0.11 mg/ml indicated that A1 posses the highest enzyme activities which were categorized under Bacillus and protein contents and A5 showed less amount of enzyme activities and protein contents as compared to other. Therefore, Bacillus subtilus should be recommended to be cultured for the production of amylase enzyme.

Keywords: Amylase, Immobilize, Bacteria, Bacillus, Culture

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Kumar , A., K. Gudeta, J. Julka, N. Kumar, and P. Sankhyan. “A, Immobilization of Amylase Producing Bacteria (Bacillus Subtilis) from Soil of Western Himalayan Region Solan, Himachal Pradesh, India”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 14, no. 2, Feb. 2021, https://innovareacademics.in/journals/index.php/ajpcr/article/view/40036.
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