A NOVEL OF BEZAFIBRATE ANALYSIS METHODS IN URINE (IN VITRO) USING SOLID PHASE EXTRACTION– HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY-UV DETECTOR


Iyan Sopyan, Widiastuty Irawati, Wiwiek Indriyati

Abstract


Objective: Bezafibrate is the second generation of fibrate groups used as the drug of choice in the treatment of hyperlipidemia. The purpose of this study is to obtained a validated method for analyzing bezafibrate in urine using solid phase extraction (SPE)-High performance liquid chromatography (HPLC).

Methods: Solid phase extraction (SPE) using hydrophilic-lipophilic balance (HLB) cartridge was performed for bezafibrate extraction from urine, afterward, a validation of analysis method using high-performance liquid chromatography (HPLC)-(UV) detection was conducted to parameters, including: selectivity (Rs), linearity (r), accuracy, precision, limit of detection (LOD) and limit of quantification (LOQ).

Results: Recovery extraction using SPE resulted %recovery 85-110%. The analysis was performed by high-performance liquid chromatography using reversed phase, C18 octadecylsilane (ODS) columns 250 x 26 mm, particle size 10 μl, with the composition of 0.01 M acetate buffer with pH 3.55: with percent composition (45:55) and 0.8 ml/minute on 230 nm UV detection. Validation includes selectivity, linearity, accuracy, precision LOD, and LOQ have fulfilled requirement value.

Conclusion: The result of recovery extraction using SPE and validation of method exhibited the values that fulfilled the requirements and can be used for analysis bezafibrate in the urine.


Keywords


Bezafibrate, HPLC, SPE, Urine

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About this article

Title

A NOVEL OF BEZAFIBRATE ANALYSIS METHODS IN URINE (IN VITRO) USING SOLID PHASE EXTRACTION– HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY-UV DETECTOR

Keywords

Bezafibrate, HPLC, SPE, Urine

DOI

10.22159/ijap.2018v10i1.22769

Date

06-01-2018

Additional Links

Manuscript Submission

Journal

International Journal of Applied Pharmaceutics
Vol 10, Issue 1 (Jan-Feb), 2018 Page: 94-97

Online ISSN

0975-7058

Statistics

90 Views | 11 Downloads

Authors & Affiliations

Iyan Sopyan
Departement of pharmaceutics and Technology of Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran
Indonesia

Widiastuty Irawati
Depertemen of Pharmaceutical analysis and Medicinal Chemistry, Faculty of Pharmacy, Universitas Padjadjaran.
Indonesia

Wiwiek Indriyati
Depertemen of Pharmaceutical analysis and Medicinal Chemistry, Faculty of Pharmacy, Universitas Padjadjaran.
Indonesia


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