EFFECT OF HYDROPHILICITY SURFACTANTS TOWARD CHARACTERIZATION AND IN VITRO TRANSFERSOMES PENETRATION IN GELS USING FRANZ DIFFUSION TEST

  • Wiranti Anggraini Department of Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Jakarta, Indonesia.
  • Erny Sagita Department of Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Jakarta, Indonesia.
  • Iskandarsyah Iskandarsyah Department of Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Jakarta, Indonesia.

Abstract

Objective: Capsaicin is a bioactive compound with poor solubility in water. Transfersomes can increase the solubility and thus the penetration of
capsaicin into the epidermis. Transfersomes also offer the advantage of being ultradeformable vesicles that are made using the thin layer hydration
method. The aim of this study is to determine the effect of the hydrophilicity of surfactants on the characterization and in vitro penetrative ability of
capsaicin transfersomes in gels using non-ionic surfactants with different hydrophile-lipophile balances, namely, Span 80, Tween 80, and a mixture
of Span 80, and Tween 80.
Methods: The best characterization result for transfersomes is the formula using Tween 80. The total cumulative amount of capsaicin penetrated
from the transfersome gel using Tween 80 is 1663.89±1.58 mg/cm2, the percentage is 57.96±0.05%, and the flux is 166.38±0.15 μg/cm2.hr−1.
Results: In terms of the transfersome gel using a mixture of Span 80 and Tween 80, the cumulative amount of capsaicin penetrated is
1539.8±21.23 μg/cm2, the percentage is 54.47±0.75%, and the flux is 153.98±2.12 μg/cm2.hr−1. Finally, from the transfersome gel using Span 80, the
cumulative amount of capsaicin penetrated is 1395.10±7.23 mg/cm2, the percentage is 50.80±0.26%, and the flux is 139.51±0.72 mg/cm2.hr−1.
Conclusions: The best characteristics resulted from the transfersome formulation using Tween 80, which demonstrated the highest entrapment
efficiency calculation result, the smallest particle size, the best deformability index, and the highest penetration when compared to the other two
formulations.

Keywords: Transfersome, Capsaicin, Non-ionic surfactants, Hydrophilicity, Transfersome gel, Penetration study.

References

1. Patel RD, Singh S, Navin S, Gendle R. Development and characterization
of curcumin loaded transfersome for transdermal delivery. J Pharm Sci
Res 2009;1(4):71-80.
2. Vinod KR, Kumar MS, Anbazhagan S, Sandhya S, Saikumar P,
Rohit RT, et al. Critical issues related to transfersomes - Novel vesicular
system. Acta Sci Pol Technol Aliment 2012;11(1):67-82.
3. Saraf S, Jeswani G, Kaur CD, Saraf S. Development of novel herbal
cosmetic cream with curcuma longa extract loaded transfersomes for
anti-wrinkle effect. Afr J Pharm Pharmacol 2011;5(8):1054-62.
4. Anwar, E. Eksipien Dalam Sediaan Farmasi Karkterisasi dan Aplikasi.
Jakarta: Dian Rakyat; 2011.
5. Jacob L, Anoop KR. A review on surfactants as edge activator in
ultradeformable vesicles for enhanced skin delivery. Int J Pharm Bio
Sci 2013;4(3):337-44.
6. Mou J, Paillard F, Turnbull B, Trudeau J, Stoker M, Katz NP. Efficacy of
Qutenza® (capsaicin) 8% patch for neuropathic pain: A meta-analysis
of the Qutenza Clinical Trials Database. Pain 2013;154:1632-9.
7. Sugiyati R, Iskandarsyah, Djajadisastra J. Formulation and in vitro
penetration evaluation of transfersome gel preparation contains caffeine
as an anticellulite. J Ilmu Kefarmasian Indones 2015;13(2):131-6.
Fig. 1: The transmission electron microscope result for the
transfersome suspension using Span 80
Fig. 2: The transmission electron microscope result for the
transfersome suspension using a Span 80 and Tween 80 mixture
Fig. 3: The transmission electron microscope result for the
transfersome suspension using Tween 80
Fig. 4: Capsaicin cumulative penetration for transfersome gel
containing Span 80, Span 80 and Tween 80 mixture, and Tween 80
115
Int J App Pharm, Special Issue (October)
Anggraini et al.
8. Chaudhary H, Kohli K, Kumar V. Nano-transfersomes as a novel carrier
for transdermal delivery. Int J Pharm 2013;454(1):367-80.
9. Kuncari ES, Iskandarsyah I, Praptiwi. Evaluation, physical stability test
and syneresis of gel containing minoxidil, apigenin and celery (Apium
greveolens L.) Juice. Bul Penelitian Kesehatan 2014;42(4):213-22.
10. Kumesan YA, Yamlean PV, Supriati HS. Formulation and activity
test of antiacne extract (Crinum asiaticum L.) toward Staphylococcus
aureus in vitro. J Ilmiah Farmasi 2013;2(2):18-26.
11. Nadia LM, Suptijah P, Bustami I. Production and characterization
chitosan nano from black tiger shrimpwith ionic gelation methods. J
Pengolahan Hasil Perikanan Indones 2014;17(2):119-26.
12. Abdallah MH. Transfersomes as a transdermal drug delivery system for
enhacement the antifungal activity of nystatin. Int J Pharm Pharm Sci
2013;5(4):560-7.
Statistics
144 Views | 244 Downloads
How to Cite
Anggraini, W., Sagita, E., & Iskandarsyah, I. (2017). EFFECT OF HYDROPHILICITY SURFACTANTS TOWARD CHARACTERIZATION AND IN VITRO TRANSFERSOMES PENETRATION IN GELS USING FRANZ DIFFUSION TEST. International Journal of Applied Pharmaceutics, 9, 112-115. https://doi.org/10.22159/ijap.2017.v9s1.67_74
Section
Original Article(s)