• Nuraini Azizah Department of Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Jakarta, Indonesia.
  • Erny Sagita Department of Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Jakarta, Indonesia.
  • Iskandarsyah Iskandarsyah Department of Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Jakarta, Indonesia.


Objective: Capsaicin is an active compound found in chili pepper and has been shown to have analgesic, antioxidant, anticancer, and anti-obesity
properties. To improve its penetration into the skin, capsaicin was prepared in a transethosome vesicle. Importantly, transethosomes are vesicles that
consist of phosphatidylcholine, surfactant, and ethanol. In this study, capsaicin was prepared in a transethosome vesicle using two different methods:
Direct transethosome formation and thin layer hydration. The aims of this study were to determine the effects of various methods of transethosome
formation on capsaicin characteristics and to evaluate the penetration capabilities of transethosome capsaicin gel.
Methods: Ultimately, transethosome formation via the thin layer method yielded more favorable characteristics; these formations had particle sizes
of 174.9±2.02 nm and an entrapment efficiency of 84.85±1.15%. The transethosome suspension was then developed into a gel formulation using 1%
carbomer. An in vitro penetration test was performed using a Franz diffusion cell of mice abdomen skin, and the performance of the transethosome
capsaicin gel was compared to that of the standard capsaicin gel.
Results: Penetration rate of capsaicin from either the transethosome gel preparation and the standard gel substance was 1549.68±49.6 and
846.05±10.1 μg/cm2, respectively.
Conclusions: According to these results, it can be concluded that gel preparations containing transethosome increase capsaicin penetration into the

Keywords: Capsaicin, Franz diffusion cell, Gel, Penetration, Transethosome.


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How to Cite
Azizah, N., Sagita, E., & Iskandarsyah, I. (2017). IN VITRO PENETRATION TESTS OF TRANSETHOSOME GEL PREPARATIONS CONTAINING CAPSAICIN. International Journal of Applied Pharmaceutics, 9, 116-119.
Original Article(s)