ACCELERATED WOUND HEALING ABILITY OF SACRAN HYDROGEL FILM BY KERATINOCYTE GROWTH FACTOR IN ALLOXAN-INDUCED DIABETIC MICE


Nasrul Wathoni, Aliya Nur Hasanah, Ahmed Fouad Abdelwahab Mohammed, Elasari Dwi Pratiwi, Ripa’atul Mahmudah

Abstract


Objective: The main objective of the research work was to fabricate sacran hydrogel film containing keratinocyte growth factor (Sacran/KGF-HGF), and to evaluate their wound healing ability in alloxan-induced diabetic mice model.

Methods: The physicochemical characterization of Sacran/KGF-HGF were investigated by thickness, tensile strength, swelling ratio, x-ray diffractometer (XRD), scanning electron microscope (SEM), and biodegradability. The wound healing ability was investigated by creating two full-thickness excisional wounds inalloxan-induced diabetic mice.

Results: The thickness, tensile strength, and swelling ratio results showed that KGF in the Sacran/KGF-HGF improved not only the thickness of sacran hydrogel film (Sacran-HGF), but also the tensile strength and swelling ability of Sacran-HGF. The XRD and SEM results confirmed that the Sacran/KGF-HGF were amorphous and similar morphology to Sacran-HGF, respectively. The biodegradability results revealed that the Sacran/KGF-HGF degraded for about 41.29% in trichloroacetic acid (TCA) and 22.92% in TrypLE™ (recombinant enzyme) solutions. In addition, KGF improved the degradability of Sacran/KGF-HGF in both solutions. Interestingly, the Sacran/KGF-HGF, which was applied on wound site, considerably improved the wound healing ability of Sacran-HGF at 6, 9 and 12 d in alloxan-induced diabetic mice model, compared to control (non-treated).

Conclusion: These results suggest that KGF has the potential to promote the chronic wound healing ability of Sacran-HGF.


Keywords


Sacran, Hydrogel film, Keratinocyte growth factor, Wound healing

| PDF | HTML |

References


Broughton GII, Janis JE, Attinger CE. The basic science of wound healing. Plast Reconstr Surg 2006;117 Suppl 7:12–34.

Sorg H, Reinke JM. Wound repair and regeneration. Eur Surg Res 2012;49:35–43.

Martin A, Komada MR, Sane DC. Abnormal angiogenesis in diabetes mellitus. Med Res Rev 2003;23:117–45.

O’Loughlin A, Kulkarni M, Creane M, Vaughan EE, Mooney EG, Shaw, et al. Topical Administration of allogeneic mesenchymal stromal cells seeded in a collagen scaffold augments wound healing and increases angiogenesis in the diabetic rabbit ulcer. Diabetes 2013;62:2588–94.

Xu F, Zhang C, Graves DT. Abnormal cell responses and role of TNF-a in impaired diabetic wound healing. Biomed Res Int 2013:1–8. http://dx.doi.org/10.1155/2013/754802.

Khatri SK, Rathman and M, Nikhila R. Formulation and evaluation of wound healing activity of linezolid topical preparations on diabetic rats. Asian J Pharm Clin Res 2016;8:30–6.

Sushanth KA, Lakshmi KC, Reddy DS. Evaluation of wound healing activity with a new formulation of dry mangiferaindica and honey using swiss albino mice. Int J Appl Pharm 2016;9:139-42.

Joao De Masi ECD, Campos ACL, Joao De Masi FD, Ratti MAS, Ike IS, Joao De Masi RD. The influence of growth factors on skin wound healing in rats. Braz J Otorhinolaryngol 2016;82:512-21.

Ram M, Singh V, Kumawat S, Kant V, Tandan SK, Kumar D. Bilirubin modulated cytokines, growth factors and angiogenesis to improve cutaneous wound healing process in diabetic rats. Int Immunopharmacol 2016;30:137–49.

Pastar I, Stojadinovic O, Yin NC, Ramirez H, Nusbaum AG, Sawaya A, et al. Epithelialization in wound healing : a comprehensive review. Adv Wound Care (New Rochelle) 2014;3:445–64.

Werner S. Keratinocyte growth factor: a unique player in epithelial repair processes. Cytokine Growth Factor Rev 1998;2:153-65.

Margolis DJ, Hoffstad O, Nafash J, Leonard CE, Freeman CP, Hennessy S, et al. Location, location, location: geographic clustering of lower-extremity amputation among medicare beneficiaries with diabetes. Diabetes Care 2011;34:2363–7.

Ovington LG. Advances in wound dressings. Clin Dermatol 2007;25:33–8.

Martin C, Low WL, Amin MCIM, Radecka I, Raj P, Kenward K. Current trends in the development of wound dressings, biomaterials and devices. Pharm Pat Anal 2013;3:341–59.

Sarabahi S. Recent advances in topical wound care. Indian J Plast Surg 2012;45:379–87.

Schoukens S. Bioactive dressings to promote wound healing. Advanced Textiles for Wound Care, A volume in Woodhead Publishing Series in Textiles; 2009. p. 114-52.

Dhivya S, Padma VV, Santhini E. Wound dressings–a review. Biomedicine 2015;5:24–8.

Mogoşanu GD, Grumezescu AM. Natural and synthetic polymers for wounds and burns dressing. Int J Pharm 2014;463:127–36.

Ko HF, Sfeir C, Kumta PN. Novel synthesis strategies for natural polymer and composite biomaterials as potential scaffolds for tissue engineering. Philos Trans A Math Phys Eng Sci 2010;368:1981–97.

Andreu V, Mendoza G, Arruebo M, Irusta S. Smart dressings based on nanostructured fibers containing natural origin antimicrobial, anti-inflammatory, and regenerative compounds. Materials (Basel) 2015;8:5154–93.

Fukushima S, Motoyama K, Tanida Y, Higashi T, Ishitsuka Y, Kondo Y, et al. Clinical evaluation of novel natural polysaccharides sacran as a skincare material for atopic dermatitis patients. J Cosmet Dermatological Sci Appl 2016;1:9-18.

Wathoni N, Motoyama K, Higashi T, Okajima M, Kaneko T, Arima H. Physically crosslinked-sacran hydrogel films for wound dressing application. Int J Biol Macromol 2016;89:465–70.

Wathoni N, Motoyama K, Higashi T, Okajima M, Kaneko T, Arima H. Enhancing effect of γ-cyclodextrin on wound dressing properties of sacran hydrogel film. Int J Biol Macromol 2017;94:181–6.

Wathoni N, Motoyama K, Higashi T, Okajima M, Kaneko T, Arima H. Enhancement of curcumin wound healing ability by complexation with 2-hydroxypropyl-g-cyclodextrin in sacran hydrogel film. Int J Biol Macromol 2017;98:268–76.

Koo H, Jin G, Kang H, Lee Y, Nam HY, Jang H, et al. A new biodegradable crosslinked polyethene oxide sulfide (PEOS) hydrogel for controlled drug release. Int J Pharm 2009;374:58–65.

Xu FJ, Kang ET, Neoh KG. pH-and temperature-responsive hydrogels from crosslinked triblock copolymers prepared via consecutive atom transfer radical polymerizations. Biomaterials 2006;27:2787–97.

Zhang Y, Zhang M, Jiang H, Shi J, Li F, Xia Y, et al. Bio-inspired layered chitosan/graphene oxide nanocomposite hydrogels with high strength and pH-driven shape memory effect. Carbohydr Polym 2017;177:116–25.

Mateescu A, Wang Y, Dostalek J, Jonas U. Thin hydrogel films for optical biosensor applications. Membranes (Basel) 2012;2:40–69.

Luo Y, Kirker KR, Prestwich GD. Cross-linked hyaluronic acid hydrogel films: new biomaterials for drug delivery. J Controlled Release 2000;69:169–84.

Kamoun EA, Kenawy ERS, Chen X. A review on polymeric hydrogel membranes for wound dressing applications: PVA-based hydrogel dressings. J Adv Res 2017;8:217–33.

Pereira R, Carvalho A, Vaz DC, Gil MH, Mendes A, Bartolo P. Development of novel alginate-based hydrogel films for wound healing applications. Int J Biol Macromol 2013;52:221–30.

Kikumoto Y, Sugiyama H, Inoue T, Morinaga H, Takiue K, Kitagawa M, et al. Sensitization to alloxan-induced diabetes and pancreatic cell apoptosis in acatalasemic mice. Biochim Biophys Acta-Mol Basis Dis 2010;1802:240–6.

Jimenez PA, Rampy MA. Keratinocyte growth factor-2 accelerates wound healing in incisional wounds. J Surg Res 1999;81:238–42.




About this article

Title

ACCELERATED WOUND HEALING ABILITY OF SACRAN HYDROGEL FILM BY KERATINOCYTE GROWTH FACTOR IN ALLOXAN-INDUCED DIABETIC MICE

Keywords

Sacran, Hydrogel film, Keratinocyte growth factor, Wound healing

DOI

10.22159/ijap.2018v10i2.24217

Date

07-03-2018

Additional Links

Manuscript Submission

Journal

International Journal of Applied Pharmaceutics
Vol 10, Issue 2 (Mar-Apr), 2018 Page: 57-61

Online ISSN

0975-7058

Statistics

174 Views | 45 Downloads

Authors & Affiliations

Nasrul Wathoni
Department of Pharmaceutics, Faculty of Pharmacy, Universitas Padjadjaran, Jatinangor 45363, Indonesia
Indonesia

Aliya Nur Hasanah
Department of Analytical Pharmacy and Medicinal Chemistry, Faculty of Pharmacy, Universitas Padjadjaran, Jatinangor 45363, Indonesia
Indonesia

Ahmed Fouad Abdelwahab Mohammed
Graduate School of Pharmaceutical Sciences, Kumamoto University, Kumamoto 862-0973, Japan
Egypt

Elasari Dwi Pratiwi
Department of Pharmaceutics, Faculty of Pharmacy, Universitas Padjadjaran, Jatinangor 45363, Indonesia

Ripa’atul Mahmudah
Department of Pharmaceutics, Faculty of Pharmacy, Universitas Padjadjaran, Jatinangor 45363, Indonesia


Refbacks

  • There are currently no refbacks.