EFFECTS OF A 980-NM DIODE LASER'S ACTIVATION OF 2.5% NAOCL AND 2% CHLORHEXIDINE ANTIFUNGAL IRRIGATION SOLUTIONS ON CANDIDA ALBICANS BIOFILMS
Keywords:Candida albicans, Diode laser, Endodontic irrigants
Objective: Candida albicans is the most frequently found fungi in persistent root canal infections; it can form a biofilm and penetrate into dentinal tubules. Endodontic irrigants, such as 2.5% sodium hypochlorite (NaOCl) and 2% chlorhexidine (CHX), have antifungal properties, but limited penetration into dentinal tubules, which somewhat protects the C. albicans fungi. The use of a diode laser is an innovative approach in root canal treatments because it is able to penetrate deeper into the dentinal tubules. This study examined the effect of a 980-nm diode laser on the antifungal properties of 2.5% NaOCl and 2% CHX on C. albicans biofilms.
Methods: The number of C. albicans colonies in the biofilms was recorded after irrigation using 2.5% NaOCl and 2% CHX. Then, the biofilms were radiated using a 980-nm diode laser.
Results: Showed statistically significant differences between the use of the irrigants only and the use of the irrigants plus the diode laser treatment in the reduction of the C. albicans colonies in the biofilm.
Conclusion: Diode laser is able to activate the antifungal properties of the 2.5% NaOCl and 2% CHX endodontic irrigants.
Garg N, Garg A, editors. Introduction and scope of endodontics. In: Textbook of Endodontics. 3rd ed. New Delhi: Jaypee Brothers Medical Publishers; 2014.
Mathew J, Emil J, Paulaian B, John B, Raja J, Mathew J. Viability and antibacterial efficacy of four root canal disinfection techniques evaluated using confocal laser scanning microscopy. J Conserv Dent 2014;17:444-8.
Jonathan R, Mathew J, Suganthan P, Samuel A, John B. Comparative evaluation of the antibacterial efficacy of four different disinfection techniques in minimally instrumented experimentally infected root canals: An in vitro study. Int J Laser Dent 2013;3:49-54.
Shenoy A, Mandava P, Bolla N, Raj S, Kurien J, Prathap MS. Antibacterial efficacy of sodium hypochlorite with a novel sonic agitation device. Indian J Dent Res 2013;24:537-41.
Onay EO, Alikaya C, Seker E. Evaluation of antifungal efficacy of erbium, chromium: Yttrium-scandium-gallium-garnet laser against Candida albicans. Photomed Laser Surg 2010;28 Suppl 1:S73-8.
Mohammadi Z, Asgary S. Antifungal activity of endodontic irrigants. Iran Endod J 2015;10:144-7.
Shenoy A, Nayak S, Bella N. An in vitro comparison of the antimicrobial effects of sodium dichloroisocyanurate, CHX and sodium hypochlorite against Enterococcus faecalis and Candida albicans. Guident 2013;6:69-74.
Siqueira JF Jr, Sen BH. Fungi in endodontic infections. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2004;97:632-41.
Garg N, Garg A. Irrigation and intracanal medicaments. In: Garg N, Garg A, editors. Textbook of Endodontics. Malaysia: Unipress Publishing; 2008.
Baz P, Biedma B, Pinon M, Mundina B, Bahillo J, Prado R, et al. Combined sodium hypochlorite and 940 nm diode laser treatment against mature e.Faecalis biofilms in vitro. J Lasers Med Sci 2012;3:116-21.
Mehrvarzfar P, Saghiri MA, Asatourian A, Fekrazad R, Karamifar K, Eslami G, et al. Additive effect of a diode laser on the antibacterial activity of 2.5% NaOCl, 2% CHX and MTAD against Enterococcus faecalis contaminating root canals: An in vitro study. J Oral Sci 2011;53:355-60.
Bago I, Plecko V, Gabric Panduric D, Schauperl Z, Baraba A, Anic I. Antimicrobial efficacy of a high-power diode laser, photo-activated disinfection, conventional and sonic activated irrigation during root canal treatment. Int Endod J 2013;46:339-47.
Kaiwar A, Usha HL, Meena N, Ashwini P, Murthy CS. The efficiency of root canal disinfection using a diode laser: In vitro study. Indian J Dent Res 2013;24:14-8.
Gerek M, Asci S, Yaylali D. Ex vivo evaluation of antibacterial effects of ND: YAG and diode lasers in root canals. Biotechnol Biotechnol Equip 2010;23:2031-4.
Sena NT, Gomes BP, Vianna ME, Berber VB, Zaia AA, Ferraz CC, et al. In vitro antimicrobial activity of sodium hypochlorite and CHX against selected single-species biofilms. Int Endod J 2006;39:878-85.
Gopikrishna V, Ashok P, Kumar AP, Narayanan LL. Influence of temperature and concentration on the dynamic viscosity of sodium hypochlorite in comparison with 17% EDTA and 2% CHX gluconate: An in vitro study. J Conserv Dent 2014;17:57-60.
Gulsahi K, Tirali RE, Cehreli SB, Karahan ZC, Uzunoglu E, Sabuncuoglu B. The effect of temperature and contact time of sodium hypochlorite on human roots infected with Enterococcus faecalis and Candida albicans. Odontology 2014;102:36-41.
Pradhan S, Kamik R. Temperature rise on external root surface during laser endodontic therapy using 940 nm diode laser: An in vitro study. Int J Laser Dent 2011;1:29-35.
Sirtes G, Waltimo T, Schaetzle M, Zehnder M. The effects of temperature on sodium hypochlorite short-term stability, pulp dissolution capacity, and antimicrobial efficacy. J Endod 2005;31:669-71.
Hmud R, Kahler WA, George R, Walsh LJ. Cavitational effects in aqueous endodontic irrigants generated by near-infrared lasers. J Endod 2010;36:275-8.