DIFFERENCES IN THE POTENTIAL MUTAGENICITY OF RESIN-, SILICONE-, AND BIOCERAMIC-BASED SEALERS ON LYMPHOCYTES: A PROTEIN EXPRESSION ANALYSIS

  • SANI SURYADARMA Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia
  • KAMIZAR . Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia
  • ENDANG SUPRASTIWI Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia
  • RATNA MEIDYAWATI Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia
  • MAIDA FITRI Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia

Abstract

Objective: The objective of this study was to compare the potential mutagenicity of resin-, silicone-, and bioceramic-based sealers on protein
expression in human lymphocytes. There has been limited research on resin-, silicone-, and bioceramic-based sealers effects on protein expression
in lymphocytes.
Methods: Nine samples of each sealer were incubated in 2 mL human blood for 1, 3, and 7 days. Then, the isolated lymphocytes are observed for
protein separation by electrophoresis method. Profile of protein bands observed and data were analyzed statistically by Kruskal–Wallis and post hoc
Mann–Whitney.
Results: Although no statistically significant differences in protein bands were observed among the resin-, silicone-, and bioceramic-based sealers
(p=0.111), there was a statistically significant difference between the resin- and silicone-based sealers on the 1st day (p=0.046) and 3rd day (p=0.046)
and between the silicone- and bioceramic-based sealers on the 1st day (p=0.046). Thus, the present study shows that there were differences in the
potential mutagenicity on the 1st day; resin was potentially more mutagenic followed by bioceramic and silicone. On the 3rd and 7th days, bioceramic
was potentially more mutagenic followed by resin and silicone.
Conclusion: The manuscript describes the study in detail and concludes that resin was potentially more mutagenic followed by bioceramic- and
silicone-based sealers.

Keywords: Root canal sealers, Bioceramic, Protein expression, Lymphocyte, Mutagenicity, Resin, Silicone

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SURYADARMA, S., ., K., SUPRASTIWI, E., MEIDYAWATI, R., & FITRI, M. (2019). DIFFERENCES IN THE POTENTIAL MUTAGENICITY OF RESIN-, SILICONE-, AND BIOCERAMIC-BASED SEALERS ON LYMPHOCYTES: A PROTEIN EXPRESSION ANALYSIS. International Journal of Applied Pharmaceutics, 11(1), 153-156. https://doi.org/10.22159/ijap.2019.v11s1.16021
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