THE ANTIBACTERIAL EFFECT OF A DIODE LASER USED AS AN ADJUNCT IRRIGANT ON CLINICAL ISOLATE OF ENTEROCOCCUS FAECALIS BIOFILM (IN VITRO)

  • Ayu Sandini Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia
  • Ratna Meidyawati Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia
  • Kamizar . Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia
  • Dewa Ayu Npa Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia

Abstract

Objective: The elimination of bacteria from the root canal has always been a problem in root canal management, and Enterococcus faecalis often found in the persistent intraradicular infections that occur after poor or unsuccessful endodontic treatments. The use of an irrigant with a diode laser adjunct eliminates this bacteria. To analyze and compare the effectiveness of a diode laser, chlorhexidine 2%, and natrium hypochlorite 2.5% on a clinical isolate of E. faecalis biofilms.

Methods: Using E. faecalis biofilms from clinical isolate were grown on microtiter well plate, incubated for 24 h and subjected to the following treatments: Natrium hypochlorite 2.5% (5 s), chlorhexidine 2% (5 s), natrium chloride 0.9% (5 s), and the irrigants with additional diode laser irradiation (980 nm, 15 Hz, 1.5 W, 3.5 J, 5 s). The antibacterial effects of the irrigants and diode laser were scored using colony form units (CFU).

Results: The clinical isolate colony of E. faecalis that was exposed to a saline solution and diode laser application had the highest score (18700 CFU/ml), while the lowest score (80,00 CFU/ml) was recorded in the group that was exposed to a chlorhexidine 2% irrigant with additional diode laser application.

Conclusion: The diode laser had an antibacterial effect on a clinical isolate of E. faecalis biofilm, and this effect was increased when it was used in addition to the application of chlorhexidine 2% and natrium hypochlorite 2.5% irrigants.
Keywords: Enterococcus faecalis, Diode laser, Chlorhexidine 2%, Natrium hypochlorite 2.5%.

References

1. Di Filippo G, Sidhu SK, Chong BS. The role of biofilms in endodontic treatment failure. Endod Prac Today 2014;8:87-103.
2. Guimarães DL, Otoch HM, Andrade CL, Ferreira CM, Rocha MM, Gomes FA. Microbiological evaluation of infected root canals and their correlation with pain. RSBO 2012;9:31-7.
3. Camargo AD. The antibacterial effects of lasers in endodontics. Roots 2012;31:8-14.
4. Zoletti GO, Carmo FL, Pereira EM, Rosado AS, Siqueira JF Jr, Santos KR. Comparison of endodontic bacterial community structures in root-canal-treated teeth with or without apical periodontitis. J Med Microbiol 2010;59:1360-4.
5. Wang QQ, Zhang CF, Chu CH, Zhu XF. Prevalence of Enterococcus faecalis in saliva and filled root canals of teeth associated with apical periodontitis. Int J Oral Sci 2012;4:19-23.
6. Akbar SM, Siti MS. Perawatan Saluran Akar: Buku Ilmu Endodontik. Mullana: Maharishi Markandeshwar University; 2009.
7. Kandaswamy D, Venkateshbabu N. Root canal irrigants. J Conserv Dent 2010;13:256-64.
8. Byström A, Sundqvist G. Bacteriologic evaluation of the efficacy of mechanical root canal instrumentation in endodontic therapy. Scand J Dent Res 1981;89:321-8.
9. Yang G, Yuan G, Yun X, Zhou X, Liu B, Wu H. Effects of two nickel-titanium instrument systems, mtwo versus ProTaper universal, on root canal geometry assessed by micro-computed tomography. J Endod 2011;37:1412-6.
10. Bago I, Plecko V, Gabric Panduric D, Schauperl Z, Baraba A, Anic I. Antimicrobial efficacy of a high-power diode laser, photo-activated disinfection, conventional and sonic activated irrigation during root canal treatment. Int Endod J 2013;46:339-47.
11. Mehrvarzfar P, Saghiri MA, Asatourian A, Fekrazad R, Karamifar K, Eslami G, et al. Additive effect of a diode laser on the antibacterial activity of 2.5% NaOCl, 2% CHX and MTAD against Enterococcus faecalis contaminating root canals: An in vitro study. J Oral Sci 2011;53:355-60.
12. Ayu NP, Bachtiar SM. Quantitave real-time PCR of cps Type 1,2 and 5 of Enterococcus faecalis and Candida albican isolated from infected root canal of subject requiring endodontic treatment. J Int Dent Med Res 2016;9:157-63.
13. Shajahan PA, Kumar PR, Hariprasad A, Mathew J, Shaji AP, Ahammed MF. Lasers: The magic wand in esthetic dentistry!! J Int Oral Health 2015;7:119-21.
14. Banerjee S, Sreya P. Low level laser therapy (LLLT)-a boon to dentistry. Guident 2015;9:62-3.
15. Beer F, Buchmair A, Wernisch J, Georgopoulos A, Moritz A. Comparison of two diode lasers on bactericidity in root canals - An in vitro study. Lasers Med Sci 2012;27:361-4.
16. Coluzzi D, Convissar RR. Laser fundamentals. In: Convissar R, editor. Principles and Practice of Laser Dentistry. 2nd ed. St. Louis: Elsevier; 2016.
17. Schulte-Lünzum R, Gutknecht N, Conrads G, Franzen R. The impact of a 940nm diode laser with radial firing tip and bare end fiber tip on Enterococcus faecalis in the root canal wall dentin of bovine teeth: An in vitro study. Photomed Laser Surg 2017;35:357-63.
18. Siqueira JF Jr. Primary endodontic infection. In: Treatment of Endodontic Infections. London: Quintessence Publishing; 2011.
19. Sedgley CM, Lennan SL, Appelbe OK. Survival of Enterococcus faecalis in root canals ex vivo. Int Endod J 2005;38:735-42.
20. Moritz A, Schoop U. Lasers in endodontics. In: Oral Laser Application. Berlin: Quintessenz Verlags-Gmbh; 2006.
21. Portenier I, Waltimo TM, Haapasalo M. Enterococcus faecalis-the root canal survivor and “star” in post-treatment disease. Endod Topics 2003;6:135-59.
Statistics
248 Views | 215 Downloads
How to Cite
Sandini, A., Meidyawati, R., ., K., & Npa, D. A. (2018). THE ANTIBACTERIAL EFFECT OF A DIODE LASER USED AS AN ADJUNCT IRRIGANT ON CLINICAL ISOLATE OF ENTEROCOCCUS FAECALIS BIOFILM (IN VITRO). International Journal of Applied Pharmaceutics, 9, 103-106. https://doi.org/10.22159/ijap.2017.v9s2.25
Section
Original Article(s)