THE ANTIBACTERIAL EFFECT OF A DIODE LASER USED AS AN ADJUNCT IRRIGANT ON CLINICAL ISOLATE OF ENTEROCOCCUS FAECALIS BIOFILM (IN VITRO)


Ayu Sandini, Ratna Meidyawati, Kamizar ., Dewa Ayu Npa

Abstract


Objective: The elimination of bacteria from the root canal has always been a problem in root canal management, and Enterococcus faecalis often found in the persistent intraradicular infections that occur after poor or unsuccessful endodontic treatments. The use of an irrigant with a diode laser adjunct eliminates this bacteria. To analyze and compare the effectiveness of a diode laser, chlorhexidine 2%, and natrium hypochlorite 2.5% on a clinical isolate of E. faecalis biofilms.

Methods: Using E. faecalis biofilms from clinical isolate were grown on microtiter well plate, incubated for 24 h and subjected to the following treatments: Natrium hypochlorite 2.5% (5 s), chlorhexidine 2% (5 s), natrium chloride 0.9% (5 s), and the irrigants with additional diode laser irradiation (980 nm, 15 Hz, 1.5 W, 3.5 J, 5 s). The antibacterial effects of the irrigants and diode laser were scored using colony form units (CFU).

Results: The clinical isolate colony of E. faecalis that was exposed to a saline solution and diode laser application had the highest score (18700 CFU/ml), while the lowest score (80,00 CFU/ml) was recorded in the group that was exposed to a chlorhexidine 2% irrigant with additional diode laser application.

Conclusion: The diode laser had an antibacterial effect on a clinical isolate of E. faecalis biofilm, and this effect was increased when it was used in addition to the application of chlorhexidine 2% and natrium hypochlorite 2.5% irrigants.

Keywords


Enterococcus faecalis, Diode laser, Chlorhexidine 2%, Natrium hypochlorite 2.5%.

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About this article

Title

THE ANTIBACTERIAL EFFECT OF A DIODE LASER USED AS AN ADJUNCT IRRIGANT ON CLINICAL ISOLATE OF ENTEROCOCCUS FAECALIS BIOFILM (IN VITRO)

Keywords

Enterococcus faecalis, Diode laser, Chlorhexidine 2%, Natrium hypochlorite 2.5%.

DOI

10.22159/ijap.2017.v9s2.25

Date

01-01-2018

Additional Links

Manuscript Submission

Journal

International Journal of Applied Pharmaceutics
Vol. 9, Special Issue 2 (Dec.), 2017 Page: 103-106

Online ISSN

0975-7058

Statistics

115 Views | 82 Downloads

Authors & Affiliations

Ayu Sandini
Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia

Ratna Meidyawati
Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia
Indonesia

Kamizar .
Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia

Dewa Ayu Npa
Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia


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