CYTOGENETIC COMPARISON OF BIOCERAMIC, SILICONE, AND METHACRYLATE RESIN SEALERS ON T LYMPHOCYTES (MICRONUCLEI ANALYSIS)

  • DEWI ISROYATI SUGIANA Department of Conservative, Faculty of Dentistry, Universitas Indonesia, Jakarta
  • KAMIZAR NAZAR Department of Conservative, Faculty of Dentistry, Universitas Indonesia, Jakarta
  • RATNA MEIDYAWATI Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia
  • SUNNY TULIM Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia

Abstract

Objective: Biocompatibility refers to the manner in which materials respond to living cells and includes cytotoxicity, cytogenicity, genotoxicity, and
carcinogenicity. To determine cytogenicity, we count the micronuclei that form after applying materials to living cells. Sealer is a chemical material
that can be directly contacted in periapical tissue and is potentially cytogenetic. Bioceramic, silicon, and methacrylate resin sealers have ingredients
that are potentially cytogenetic. We examined the interactions of these sealers with lymphocyte T-cells.
Methods: We counted the number of micronuclei following treatment with bioceramic, silicone, and methacrylate resin sealers on lymphocyte T-cells
at 1, 3, and 7 days.
Results: The micronuclei scores associated with bioceramic and silicone sealers were lower than methacrylate resin (p<0.05) between days 1, 3, and 7.
The micronuclei scores of bioceramic and silicone sealers on day 1 were higher than on days 3 and 7. There were no significant between-group
differences for bioceramic and silicone sealers on days 3 and 7. The highest micronuclei score for methacrylate resin was on day 1.
Conclusion: Bioceramic and silicone sealers were less cytogenetic than methacrylate resin sealer. However, all of the sealers produce micronuclei on
days 1, 3, and 7.

Keywords: Cytogenetic, Bioceramic, Silicone, Methacrylate resin, Micronuclei

References

1. Lin LM, Rosenberg PA. Repair and regeneration in endodontics. Int
Endod J 2011;44:889-906.
2. Hargreaves K, Berman L. Cohen’s Pathways of the Pulp Expert
Consult. 11th ed. Maryland Heights (MO): Mosby; 2015.
3. Candeiro GT, Moura-Netto C, D’Almeida-Couto RS, Azambuja-
Junior N, Marques MM, Cai S, et al. Cytotoxicity, genotoxicity and
antibacterial effectiveness of bioceramic endodontic siler. Int Endontic
J 2016;49:858-64.
4. da Silva PT, Pappen FG, Souza EM, Dias JE, Bonetti Filho I, Carlos IZ,
et al. Cytotoxicity evaluation of four endodontic sealers. Braz Dent J 2008;
19:228-31.
5. Hintzsche H, Hemmann U, Poth A, Utesch D, Lott J, Stopper H,
et al. Fate of micronuclei and micronucleated cells. Mutat Res 2017;
771:85?98.
6. Chang SW, Lee SY, Kang SK, Kum KY, Kim EC. In vitro
biocompatibility, inflammatory response, and osteogenic potential of
4 root canal sealers: Sealapex, sankin apatite root sealer, MTA fillapex,
and iRoot SP root canal sealer. J Endod 2014;40:1642-8.
7. IAEA. Cytogenetic Dosimetry: Application in Preparedness for and
Responce to Radiation Emergencies. Vienna: IAEA; 2011.
8. Silva EJ, Santos CC, Zaia AA. Long-term cytotoxic effects of
contemporary root canal sealers. J Appl Oral Sci 2013;21:43-7.
9. Zhou HM, Du TF, Shen Y, Wang ZJ, Zheng YF, Haapasalo M, et al.
In vitro cytotoxicity of calcium silicate-containing endodontic sealers.
J Endod 2015;41:56-61.
10. Pameijer CH, Zmener O. Resin materials for root canal obturation.
Dent Clin North Am 2010;54:325-44.
11. Zhou HM, Shen Y, Zheng W, Li L, Zheng YF, Haapasalo M, et al.
Physical properties of 5 root canal sealers. J Endod 2013;39:1281-6.
12. Pawi?ska M, ?uczaj-Cepowicz E, Kierklo A, Marczuk-Kolada G,
Ho?ownia A. Assessment of cytotoxic potential of root canal sealers
after hardening-an ex vivo study. Postepy Hig Med Dosw (Online) 2015;
69:503-9.
13. Zoufan K, Jiang J, Komabayashi T, Wang YH, Safavi KE, Zhu Q, et al.
Cytotoxicity evaluation of gutta flow and endo sequence BC sealers.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2011;112:657-61.
14. Al-Haddad A, Che Ab Aziz ZA. Bioceramic-based root canal sealers:
A review. Int J Biomater 2016;2016:9753210.
15. Collado-González M, Tomás-Catalá CJ, Oñate-Sánchez RE,
Moraleda JM, Rodríguez-Lozano FJ. Cytotoxicity of guttaFlow bioseal,
guttaFlow2, MTA fillapex, and AH plus on human periodontal ligament
stem cells. J Endod 2017;43:816-22.
16. Saygili G, Saygili S, Tuglu I, Davut Capar I. In vitro cytotoxicity of
guttaFlow bioseal, guttaFlow 2, AH-plus and MTA fillapex. Iran Endod
J 2017;12:354-9.
17. Vitti RP, Prati C, Sinhoreti MA, Zanchi CH, Souza E Silva MG,
Ogliari FA, et al. Chemical-physical properties of experimental root
canal sealers based on butyl ethylene glycol disalicylate and MTA. Dent
Mater 2013;29:1287-94.
18. Loushine BA, Bryan TE, Looney SW, Gillen BM, Loushine RJ,
Weller RN, et al. Setting properties and cytotoxicity evaluation of a
premixed bioceramic root canal sealer. J Endod 2011;37:673-7.
19. Victoria-Escandell A, Ibañez-Cabellos JS, de Cutanda SB, Berenguer-
Pascual E, Beltrán-García J, García-López E, et al. Cellular responses
in human dental pulp stem cells treated with three endodontic materials.
Stem Cells Int 2017;2017:8920356.
20. Thrikivaman G, Madras G, Basu B. In vitro assessment and mechanisms
of toxicity of bioceramic materials and its wear particulates. RSC
Adv 2014;4:12763-81.
21. Corrêa JM, Mori M, Sanches HL, da Cruz AD, Poiate E Jr.,
Poiate IA, et al. Silver nanoparticles in dental biomaterials. Int J
Biomater 2015;2015:485275.
22. McShan D, Ray PC, Yu H. Molecular toxicity mechanism of nanosilver.
J Food Drug Anal 2014;22:116-27.
23. Feng X, Chen A, Zhang Y, Wang J, Shao L, Wei L, et al. Application of
dental nanomaterials: Potential toxicity to the central nervous system.
Int J Nanomedicine 2015;10:3547-65.
24. Zhang XF, Liu ZG, Shen W, Gurunathan S. Silver nanoparticles:
Synthesis, characterization, properties, applications, and therapeutic
approaches. Int J Mol Sci 2016;17:1534.
Statistics
84 Views | 80 Downloads
Citatons
How to Cite
SUGIANA, D. I., NAZAR, K., MEIDYAWATI, R., & TULIM, S. (2019). CYTOGENETIC COMPARISON OF BIOCERAMIC, SILICONE, AND METHACRYLATE RESIN SEALERS ON T LYMPHOCYTES (MICRONUCLEI ANALYSIS). International Journal of Applied Pharmaceutics, 11(1), 146-149. https://doi.org/10.22159/ijap.2019.v11s1.16014
Section
Original Article(s)