• THERESIA PEGGY HARYANTI Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia
  • KAMIZAR NAZAR Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia
  • ANGGRAINI MARGONO Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia



Antibacterial efficacy, Biofilm, Chlorhexidine, Enterococcus faecalis, Nisin, Sodium hypochlorite


Objective: This study aimed to compare the antibacterial efficacy of 10% nisin, 2% chlorhexidine (ChX), and 2.5% sodium hypochlorite (NaOCl)
against Enterococcus faecalis biofilm in vitro.
Methods: Petri dishes containing brain heart infusion agar were seeded with E. faecalis (ATCC 29212) and were incubated overnight at 37°C. The
cellulose nitrate filter membrane was inoculated with E. faecalis for 72 h to grow a biofilm, and we performed the direct contact test between the test
solutions and the biofilm for 10 min. The DNA was quantified using real-time polymerase chain reaction with propidium monoazide additive to count
the living cells.
Results: The number of E. faecalis bacteria in the 2% ChX group was the lowest (8.36×103 CFU/mL) while the highest number of bacteria - among the
antibacterial substances tested - in the nisin 10% group (5.55×106 CFU/mL).
Conclusion: The antibacterial effects against E. faecalis biofilm of 10% nisin were not comparable with those of 2% ChX and 2.5% NaOCl.


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