EFFECT OF TRIPLE ANTIBIOTIC PASTE, CALCIUM HYDROXIDE, LEDERMIX® ON VIABILITY OF PULP MESENCHYMAL STEM CELLS

  • EMIRIA DITA PRASANTI Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia
  • ANGGRAINI MARGONO Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia
  • NILAKESUMA DJAUHARIE Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia

Abstract

Objective: The goal of regenerative endodontic therapy is biological healing of the pulp tissue. It involves the disinfection of the canals with irrigants
and medicaments. The medicaments that are currently used for this purpose are a triple antibiotic paste (TAP), calcium hydroxide (Ca[OH]2), and
Ledermix®, a paste containing demeclocycline and triamcinolone. Therefore, the purpose of this study was to evaluate the effects of TAP, Ca(OH)2, and
Ledermix® on the viability of dental pulp stem cells (DPSC).
Methods: Primary cultures of DPSC were obtained from immature third molars. Immunofluorescence assay using STRO-1 marker was performed
to confirm the mesenchymal nature of the DPSC. The cells were exposed to TAP, Ca(OH)2, and Ledermix® at concentrations of 0.1 and 1 mg/mL. Cell
viability was analyzed using the MTT assay.
Results: Significant differences in viability were noted between the cells exposed to the medicaments and those in the control group (p<0.05).
Conclusions: All three medicaments decreased the viability of DPSC, with the Ledermix® paste demonstrating the highest toxic effect.

Keywords: Dental pulp stem cells, Triple antibiotic paste, Calcium hydroxide, Ledermix®, Cell viability

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PRASANTI, E. D., MARGONO, A., & DJAUHARIE, N. (2019). EFFECT OF TRIPLE ANTIBIOTIC PASTE, CALCIUM HYDROXIDE, LEDERMIX® ON VIABILITY OF PULP MESENCHYMAL STEM CELLS. International Journal of Applied Pharmaceutics, 11(1), 49-53. https://doi.org/10.22159/ijap.2019.v11s1.164
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