CUMINUM CYMINUM EXTRACT REDUCES DENTAL PULP STEM CELL VIABILITY AT HIGHER CONCENTRATION

  • LIPUR KURNIAWATI Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia
  • ZAAL HAQ M Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia
  • ANGGRAINI MARGONO Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia
  • DEWA AYU NYOMAN PUTRI ARTININGSIH Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia

Abstract

Objective: Cuminum cyminum (cumin) extract has potential antibacterial and antifungal activities and is not toxic for mouse fibroblasts. However, to
our knowledge, no research exists investigating the toxicity of cumin extract on dental pulp stem cells (DPSCs). Therefore, we compared the viability
of DPSCs after treatment with different doses of the cumin extract (0.1, 0.4, 0.7, and 1.0 mg/mL) at 1 h, 24 h, and 4 days.
Methods: DPSCs were gently evacuated from exfoliated third molars. Subsequently, cumin seeds were extracted by steam distillation to
obtain 0.1, 0.4, 0.7, and 1.0 mg/mL concentrations. Then, the cell viability of DPSCs was analyzed using the [3-(4,5-dimethylthiazol-2-yl)-2,5-
diphenyltetrazolium bromide] assay by calculating the absorbance values using a microplate reader, with the optical density (OD) as the final
result.
Results: There were statistically significant differences in the viability of DPSCs (p<0.05) between 0.1 and 0.4, 0.7, and 1.0 mg/mL cumin extract, but
there was no difference (p≥0.05) in the viability of DPSCs at 1 h in each group.
Conclusion: The cumin extract at all concentrations did not affect the viability of DPSCs at 1 h. However, the cumin extract at 0.4, 0.7, and 1.0 mg/mL
decreased the viability of DPSCs at 24 h and 4 days.

Keywords: Toxicity, Cell viability, Cuminum cyminum, Dental pulp stem cells

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How to Cite
KURNIAWATI, L., M, Z. H., MARGONO, A., & ARTININGSIH, D. A. N. P. (2019). CUMINUM CYMINUM EXTRACT REDUCES DENTAL PULP STEM CELL VIABILITY AT HIGHER CONCENTRATION. International Journal of Applied Pharmaceutics, 11(1), 150-152. https://doi.org/10.22159/ijap.2019.v11s1.16015
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Original Article(s)