CUMINUM CYMINUM EXTRACT REDUCES DENTAL PULP STEM CELL VIABILITY AT HIGHER CONCENTRATION
Keywords:Toxicity, Cell viability, Cuminum cyminum, Dental pulp stem cells
Objective: Cuminum cyminum (cumin) extract has potential antibacterial and antifungal activities and is not toxic for mouse fibroblasts. However, to
our knowledge, no research exists investigating the toxicity of cumin extract on dental pulp stem cells (DPSCs). Therefore, we compared the viability
of DPSCs after treatment with different doses of the cumin extract (0.1, 0.4, 0.7, and 1.0 mg/mL) at 1 h, 24 h, and 4 days.
Methods: DPSCs were gently evacuated from exfoliated third molars. Subsequently, cumin seeds were extracted by steam distillation to
obtain 0.1, 0.4, 0.7, and 1.0 mg/mL concentrations. Then, the cell viability of DPSCs was analyzed using the [3-(4,5-dimethylthiazol-2-yl)-2,5-
diphenyltetrazolium bromide] assay by calculating the absorbance values using a microplate reader, with the optical density (OD) as the final
Results: There were statistically significant differences in the viability of DPSCs (p<0.05) between 0.1 and 0.4, 0.7, and 1.0 mg/mL cumin extract, but
there was no difference (p≥0.05) in the viability of DPSCs at 1 h in each group.
Conclusion: The cumin extract at all concentrations did not affect the viability of DPSCs at 1 h. However, the cumin extract at 0.4, 0.7, and 1.0 mg/mL
decreased the viability of DPSCs at 24 h and 4 days.
1st ed. Yogyakarta: Graha Ilmu; 2011.
2. Hristov K, Gateva N, Stanimirov P. Influence of irrigants and intracanal
medicaments on the vitality of stem cells from apical papilla - An
overview. Int J Sci Res 2017;6:999-1002.
3. Petters OA, Peters C, Basrani B. Cleaning and shaping the root canal
system. In: Hargreaves KM, Berman LH, Rotstein I, editors. Cohen
Pathways of the Pulp. 11th ed. Canada: Elsevier; 2016. p. 209-78.
4. Simon S, Smith AJ. Regenerative endodontics. Br Dent J 2014;216:E13.
5. Patel AB. Dental stem cells and their potential role in regenerative
dentistry. Int J Dent Heal Sci 2015;2:852-61.
6. Taheri JB, Azimi S, Rafieian N, Zanjani HA. Herbs in dentistry. Int
Dent J 2011;61:287-96.
7. Aly LA. Stem cells: Sources, and regenerative therapies in dental
research and practice. World J Stem Cells 2015;7:1047-53.
8. Liu WJ. Traditional Herbal Medicine Research Methods. Singapore:
John Wiley and Sons, Inc.; 2011.
9. General Guidelines for Methodologies on Research and Evaluation of
Traditional Medicine World Health Organization; 2000.
10. Laredo-Naranjo MA, Carrillo-Gonzalez R, De La Garza-Ramos MA,
Garza-Navarro MA, Torre-Martinez HH, Del Angel-Mosqueda C, et al.
Antimicrobial properties and dental pulp stem cell cytotoxicity using
carboxymethyl cellulose-silver nanoparticles deposited on titanium
plates. Acta Biomater Odontol Scand 2016;2:60-7.
11. Abbaszadegan A, Gholami A, Ghahramani Y, Ghareghan R,
Ghareghan M, Kazemi A, et al. Antimicrobial and cytotoxic activity
of cuminum cyminum as an intracanal medicament compared to
chlorhexidine gel. Iran Endod J 2016;11:44-50.
12. Service M. Assessing biocompatibility. In: Kucklick TR, editor. The
Medical Device R and D Handbook. 2nd ed. Boca Raton, Florida: CRC
Press Taylor and Francis Group; 2013.
13. ISO Standard 1993. Biological Evaluation of Medical Devices - Parts
1 – 2; 1993.
14. Johri RK. Cuminum cyminum and carum carvi: An update. Pharmacogn
15. Al-Snafi AE. The pharmacological activities of Cuminum cyminum - A
review. IOSR J Pharm 2016;6:2250-3013.
16. Kamble VA. In vitro anti-fungal activity of Cuminum cyminum (Cumin
Seed) essential oil against clinical isolates of candida species. Am J
Phytomed Clin Ther 2015;3:264-75.
17. Romagnoli C, Andreotti E, Maietti S, Mahendra R, Mares D. Antifungal
activity of essential oil from fruits of indian Cuminum cyminum. Pharm
18. Kaur D, Sharma R. An update on pharmacological properties of cumin.
Int J Res Pharm Sci 2012;2:14-27.
19. LoPachin RM, Gavin T. Molecular mechanisms of aldehyde toxicity:
A chemical perspective. Chem Res Toxicol 2014;27:1081-91.