• PRISCILLA ARLYTA SIMANJUNTAK Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia.
  • NILAKESUMA DJAUHARIE Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia.
  • BAMBANG NURSASONGKO Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia.



Chitosan, Chlorhexidine, Enterococcus faecalis, Real-time polymerase chain reaction


Objective: Enterococcus faecalis can form biofilms and has a major role in the etiology of persistent lesions after root canal. We analyzed the efficacy
of chitosan and chlorhexidine against E. faecalis in biofilms.
Methods: Polymerase chain reaction was used to analyze E. faecalis DNA that survived and lived after immersing the biofilm in an antibacterial
Results: A statistically significant difference was noted in living E. faecalis between chitosan and control and between 2% chlorhexidine and control
groups (p≤0.05). No significant difference was noted between chitosan and chlorhexidine groups (p>0.05).
Conclusions: Antibacterial effectivity of chitosan is equal to that of chlorhexidine against E. faecalis in biofilm.


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