EVALUATION OF REGENERATIVE THERAPY USING CELL SHEET THROUGH CEMENTUM PROTEIN-1 EXPRESSION ON MACACA NEMESTRINA


Syanti Wahyu Astuty, Hari Sunarto, Lisa Amir, Erik Idrus

Abstract


Objective: The main objective of periodontal therapy is tissue regeneration. Previous studies have identified the potential of mesenchymal stem cells to improve major periodontal defect reconstruction in bone tissue engineering. Cell sheet technology (CST), in which a cell culture is obtained from a material coated with a temperature-sensitive substrate, has been developed for the reconstruction of various tissues, including periodontal tissue. Cementum protein-1 (CEMP-1) is a 50-kDa protein that plays a crucial role in cementogenesis by enhancing the combining of cells formed by cell cementoblast. Evaluate periodontal tissue regeneration with CST, and the application of chitosan, chitosan cell sheet or arginineglycineaspartic acid (RGD)-modified chitosan cell sheet on Macaca nemestrina one-wall defect model.

Methods: The CEMP-1 was analyzed expression in a sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gel electrophoresis assay.

Results: CEMP-1 expression in gingival crevicular fluid was observed using the SDS-PAGE method every week for 3 weeks. Protein band expressions on SDS-PAGE gel were identified at around 50 kDa with different thicknesses between groups. The chitosan, chitosan cell sheet, and RGD-modified chitosan cell sheet groups showed protein bands of CEMP-1 between 50 and 70 kDa at weeks 1, 2, and 3; weeks 2 and 3; and weeks 1 and 2, respectively.

Conclusion: Our results demonstrated that the application of chitosan and RGD-modified chitosan cell sheets could enhance bone regeneration, as evidenced by CEMP-1 protein expression levels.


Keywords


Periodontitis, Bone defect, Cell sheet, Cementum protein-1

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About this article

Title

EVALUATION OF REGENERATIVE THERAPY USING CELL SHEET THROUGH CEMENTUM PROTEIN-1 EXPRESSION ON MACACA NEMESTRINA

Keywords

Periodontitis, Bone defect, Cell sheet, Cementum protein-1

DOI

10.22159/ijap.2017.v9s2.26

Date

01-01-2018

Additional Links

Manuscript Submission

Journal

International Journal of Applied Pharmaceutics
Vol. 9, Special Issue 2 (Dec.), 2017 Page: 107-109

Online ISSN

0975-7058

Statistics

94 Views | 90 Downloads

Authors & Affiliations

Syanti Wahyu Astuty
Department of Periodontology, Faculty of Dentistry, Jakarta, Universitas Indonesia, Indonesia

Hari Sunarto
Department of Periodontology, Faculty of Dentistry, Jakarta, Universitas Indonesia, Indonesia

Lisa Amir
Department of Oral Biology, Faculty of Dentistry, Jakarta, Universitas Indonesia, Indonesia

Erik Idrus
Department of Oral Biology, Faculty of Dentistry, Jakarta, Universitas Indonesia, Indonesia
Indonesia


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