TOXICITY ANALYSIS OF RGD-CHITOSAN FROM SHRIMP SHELL SCAFFOLD MEMBRANES TOWARD HUMAN DENTAL PULP CELLS


Mauldina Shabrina, Dewi Fatma Suniarti, Lisa R Amir, Erik Idrus

Abstract


Objective: This study aimed to analyze RGD-Chitosan from Shrimp Shells’ Scaffolds’ (RCSSS) and CSSS membrane toxicity toward human dental pulp
cells.
Methods: Human dental pulp cells were cultured for 5 days and then exposed to RCSSS or CSSS membranes for 24 hrs. Cell viability was determined
using an MTT assay method.
Results: Cell viability of the RCSSS group and CSSS group was higher than the cell viability of the control group. The cell viability of the RCSSS
group 2 mg (537.39%) was significantly higher than the CSSS group 2 mg (301.74%).
Conclusions: RCSSS membranes were not toxic toward human dental pulp cells and showed better effect toward human dental pulp cells compared
to CSSS membranes.


Keywords


Chitosan, Human dental pulp cells, RGD-chitosan, Toxicity.

| PDF |

References


Ge S, Zhao N, Wang L, Yu M, Liu H, Song A, et al. Bone repair by

periodontal ligament stem cell-seeded nanohydroxyapatite-chitosan

scaffold. Int J Nanomed 2012;7:5405-14.

Arca HÇ, Şenel S. Chitosan based systems for tissue engineering part 1:

Hard tissues. Fabad J Pharm Sci 2008;33(1):35-49.

Amini AR, Laurencin CT, Nukavarapu SP. Bone tissue engineering:

Recent advances and challenges. Crit Rev Biomed Eng

;40(5):363-408.

Rodríguez-Vázquez M, Vega-Ruiz B, Ramos-Zúñiga R,

Saldaña-Koppel DA, Quiñones-Olvera LF. Chitosan and its potential

use as a scaffold for tissue engineering in regenerative medicine.

Biomed Res Int 2015;2015:821279.

Huang GT, Gronthos S, Shi S. Mesenchymal stem cells derived from

dental tissues vs. Those from other sources: Their biology and role in

regenerative medicine. J Dent Res 2009;88(9):792-806.

Amir LR, Suniarti DF, Utami S, Abbas B. Chitosan as a potential

osteogenic factor compared with dexamethasone in cultured macaque

dental pulp stromal cells. Cell Tissue Res 2014;358(2):407-15.

Soeroso Y, Bachtiar EW, Bachtiar BM, Sulijaya B, Prayitno SW. The

prospect of chitosan on the osteogenesis of periodontal ligament stem

cells. J Int Dent Med Res 2016;9(2):93-7.

Saravanan S, Leena RS, Selvamurugan N. Chitosan based biocomposite

scaffolds for bone tissue engineering. Int J Biol Macromol

;93:1354-65.

Dash M, Chiellini F, Ottenbrite RM, Chiellini E. Chitosan - A versatile

semi-synthetic polymer in biomedical applications. Prog Polym Sci

;36(8):981-1014.

Graziano A, d’Aquino R, Laino G, Papaccio G. Dental pulp stem cells:

A promising tool for bone regeneration. Stem Cell Rev 2008;4(1):21-6.

Tsai WB, Chen YR, Liu HL, Lai JY. Fabrication of UV-crosslinked

chitosan scaffolds with conjugation of RGD peptides for bone tissue

engineering. Carbohydr Polym 2011;85(1):129-37.

Riahi N, Liberelle B, Henry O, De Crescenzo G. Impact of RGD

amount in dextran-based hydrogels for cell delivery. Carbohydr Polym

;161:219-27.

Bellis SL. Advantages of RGD peptides for directing cell association

with biomaterials. Biomaterials 2011;32(18):4205-10.

Tsai WB, Chen YR, Li WT, Lai JY, Liu HL. RGD-conjugated UVcrosslinked

chitosan scaffolds inoculated with mesenchymal stem cells

for bone tissue engineering. Carbohydr Polym 2012;89(2):379-87.

Wang MO, Etheridge JM, Thompson JA, Vorwald CE, Dean D,

Fisher JP. Evaluation of the in vitro cytotoxicity of cross-linked

biomaterials. Biomacromolecules 2013;14(5):1321-9.

O’Brien FJ. Biomaterials and scaffolds for tissue engineering. Mater

Today 2011;14(3):88-95.

Zahra J. Chitosan: A brief review on structure and tissue engineering

application. J Appl Tissue Eng 2014;1(1):3-7.

Zhu W, Liang M. Periodontal ligament stem cells: Current status,

concerns, and future prospects. Stem Cells Int 2015;2015:972313.

Pisciotta A, Carnevale G, Meloni S, Riccio M, De Biasi S, Gibellini L,

et al. Human dental pulp stem cells (hDPSCs): Isolation, enrichment

and comparative differentiation of two sub-populations. BMC Dev Biol

;15:14.

Wang Y, Lu LX, Feng ZQ, Xiao ZD, Huang NP. Cellular compatibility

of RGD-modified chitosan nanofibers with aligned or random

orientation. Biomed Mater 2010;5(5):50201.

Enrique D, Jos P, Sobral A, Norte C. The effect of processing parameters

and solid concentration on the microstructure and pore architecture

of gelatin-chitosan scaffolds produced by freeze-drying. Mater Res

;19(4):839-45.




About this article

Title

TOXICITY ANALYSIS OF RGD-CHITOSAN FROM SHRIMP SHELL SCAFFOLD MEMBRANES TOWARD HUMAN DENTAL PULP CELLS

Keywords

Chitosan, Human dental pulp cells, RGD-chitosan, Toxicity.

DOI

10.22159/ijap.2017.v9s1.07_08

Date

30-10-2017

Additional Links

Manuscript Submission

Journal

International Journal of Applied Pharmaceutics
Vol. 9, Special Issue (Oct.), 2017 [PTMDS 2017] Page: 13-16

Online ISSN

0975-7058

Statistics

10 Views | 23 Downloads

Authors & Affiliations

Mauldina Shabrina
Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia.
Indonesia

Dewi Fatma Suniarti
Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia.
Indonesia

Lisa R Amir
Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia.
Indonesia

Erik Idrus
Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia.
Indonesia


Refbacks

  • There are currently no refbacks.