EFFECT OF CENTELLA ASIATICA L. ON THE NUMBER OF OSTEOCLASTS, OSTEOBLASTS, AND OSTEOCYTES IN THE TIBIAE OF OVARIECTOMIZED RATS

  • VANISSA KARIS Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia
  • SRI ANGKY SOEKANTO Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia
  • ERIK IDRUS Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia

Abstract

Objective: This study aimed to examine the effectiveness of Centella asiatica L. as a phytoestrogen.
Methods: Therapy using C. asiatica extract at doses of 60 mg/kg body weight, 120 mg/kg BW, and 180 mg/kg BW was administered to ovariectomized
rats for 30 days.
Results: Histological examination using hematoxylin and eosin staining demonstrated an increased number of osteocytes and a decreased number of
osteoclasts, but there was no significant increase in osteoblast numbers.
Conclusion: The administration of the low doses of C. asiatica extract affects the number of osteocytes and osteoclasts but not osteoblasts in
ovariectomized rats.

Keywords: Centella asiatica, Estrogen, Osteoporosis, Osteocytes, Osteoclasts, Osteoblasts

References

1. Glaser DL, Kaplan FS. Osteoporosis. Definition and clinical
presentation. Spine (Phila Pa 1976) 1997;22:12S-16S.
2. Martono B, Ghulamahdi M, Darusman LK, Aziz SA, Bermawie N.
Criteria for markers of cultivation and asiaticoside productivity
selection of Centella asiatica L. J Littr 2010;16:12-9.
3. Ismal NM. Postmenopausal osteoporosis: Epidemiology,
pathophysiology and treatment. Malays J Pathol 1997;19:21-5.
4. Schart D. Phytoestrogens for menopausal. Nutr Action 2000;84:717.
5. Barnes J, Anderson L, Phillipson J. Herbal medicines. Pharm Press
2007;3:71-3.
6. Zheng CJ, Qin LP. Chemical components of Centella asiatica and their
bioactivities. Zhong Xi Yi Jie He Xue Bao 2007;5:348-51.
7. Subban R, Veerakumar A, Manimaran R, Hashim KM, Balachandran I.
Two new flavonoids from Centella asiatica (Linn.). J Nat Med
2008;62:369-73.
8. Lane NE. Epidemiology, etiology, and diagnosis of osteoporosis. Am J
Obstet Gynecol 2006;194:S3-11.
9. Guo AJ, Choi RC, Zheng KY, Chen VP, Dong TT, Wang ZT, et al.
Kaempferol as a flavonoid induces osteoblastic differentiation via
estrogen receptor signaling. Chin Med 2012;7:10.
10. Endang L. Phytoestrogens: Safe Natural Compounds as a Substitute for
Estrogen Hormones in Women. Yogyakarta: Universitas Gajah Mada; 2011.
11. Choi EM, Hwang JK. Effects of (+)-catechin on the function of
osteoblastic cells. Biol Pharm Bull 2003;26:523-6.
12. Yang YJ, Yang ZL, Wang DC, Xiao XC, Li P. Comparative study on
effects of rutin and quercetin on metabolism in osteoblast cells. Zhong
Yao Cai 2006;29:467-70.
13. Nakamura T, Imai Y, Matsumoto T, Sato S, Takeuchi K, Igarashi K,
et al. Estrogen prevents bone loss via estrogen receptor alpha and
induction of fas ligand in osteoclasts. Cell 2007;130:811-23.
14. Krum SA, Miranda-Carboni GA, Hauschka PV, Carroll JS, Lane TF,
Freedman LP, et al. Estrogen protects bone by inducing fas ligand in
osteoblasts to regulate osteoclast survival. EMBO J 2008;27:535-45.
15. Cenci S, Weitzmann MN, Roggia C, Namba N, Novack D, Woodring J,
et al. Estrogen deficiency induces bone loss by enhancing T-cell
production of TNF-alpha. J Clin Invest 2000;106:1229-37.
16. Skibola CF, Smith MT. Potential health impacts of excessive flavonoid
intake. Free Radic Biol Med 2000;29:375-83.
17. Almeida M, Martin-Millan M, Ambrogini E, Bradsher R 3rd,
Han L, Chen XD, et al. Estrogens attenuate oxidative stress and
the differentiation and apoptosis of osteoblasts by DNA-bindingindependent
actions of the ERalpha. J Bone Miner Res 2010;25:769-81.
18. Wattel A, Kamel S, Mentaverri R, Lorget F, Prouillet C, Petit JP, et al.
Potent inhibitory effect of naturally occurring flavonoids quercetin
and kaempferol on in vitro osteoclastic bone resorption. Biochem
Pharmacol 2003;65:35-42.
19. Bakhsh A, Mustapha NM, Mohamed S. Catechin-rich oil palm leaf
extract enhances bone calcium content of estrogen-deficient rats.
Nutrition 2013;29:667-72.
20. Dang ZC, Lowik C. Dose-dependent effects of phytoestrogens on bone.
Trends Endocrinol Metab 2005;16:207-13.
21. Umland EM, Cauffield JS, Kirk JK, Thomason TE. Phytoestrogens
as therapeutic alternatives to traditional hormone replacement in
postmenopausal women. Pharmacotherapy 2000;20:981-90.
22. Graham JM, Ayati BP, Holstein SA, Martin JA. The Role of Osteocytes
in Targeted Bone Remodeling: A Mathematical Model 2013; 8:5-
e63884.
23. Rakhman L, Pangestika O, Fitriani D, Rudhanton R, Permatasari N.
Aloe vera ethanol extract as a therapy for alveolar mandible regeneration
in lipopolysaccharide-exposed rats. 2018;10:11-4.
24. Sivamaruthi B, Kesika P. Chaiyasut C. A Review on anti-aging
properties of probiotics. International Journal of Applied Pharmaceutics
2018;10(5):23-7.
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KARIS, V., SOEKANTO, S. A., & IDRUS, E. (2019). EFFECT OF CENTELLA ASIATICA L. ON THE NUMBER OF OSTEOCLASTS, OSTEOBLASTS, AND OSTEOCYTES IN THE TIBIAE OF OVARIECTOMIZED RATS. International Journal of Applied Pharmaceutics, 11(1), 71-74. https://doi.org/10.22159/ijap.2019.v11s1.AR171
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