THE POTENTIAL OF SEVERAL FLUORIDE-BASED VARNISHES AS REMINERALIZATION AGENTS: MORPHOLOGICAL STUDIES, DENTIN SURFACE HARDNESS, AND CRYSTALLINITY TESTS


Sri Angky Soekanto, Fadiza Fadillah, Putri Nuraisiya, Ferry Gultom, Agoeng T Sarwono

Abstract


Objectives: This study was conducted to analyze the effects of several fluoride-based varnishes: Sodium diamine fluoride (SDF), nanosilver fluoride (NSF), and propolis fluoride (PPF). Fluoride-based varnish is proven to remineralize and arrest caries progression with minimal side effects. SDF, the gold standard for this study, compared to NSF and PPF to observe the dentin morphology, hardness, and crystallinity test.

Methods: Dentin blocks were allocated and divided into control groups and treatment groups with SDF (38%), NSF (3.16%, 3.66%, and 4.16%), and PPF (3%, 6%, and 10%). The dentin discs were demineralized using a demineralization solution (pH 4.4) for 96 h. Each dentin disc in the treatment group received a topical application of various fluoride-based varnishes and was then subjected to pH cycling for 8 days. The surface morphology of dentin was observed by a scanning electron micrograph (SEM), dentin hardness was analyzed by a Vickers microhardness tester, and the crystal characteristics of dentin blocks were investigated by X-ray diffraction (XRD).

Results: This study showed crystal fluorapatite on the dentin surface of SDF and NSF. There was an amorphous layer on the dentin surface of PPF (10%) detected when using SEM. The Vickers hardness number of dentin increased after the application of SDF, NSF, and PPF. XRD revealed precipitates of apatite compounds, calcium compounds, and metal compounds in the treatment groups in higher concentrations than in the control (demineralized) group.

Conclusion: It was concluded that SDF, NSF, and PPF were effective for dentin remineralization. Hence, NSF and PPF fluoride-based varnishes are promising as anticariogenic agents.


Keywords


Nanosilver fluoride, Propolis fluoride, Silver diamine fluoride, Remineralization, Dentin morphology, Crystallinity test, Dentine hardness

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References


Kementerian Kesehatan RI. Infodatin: Situasi Kesehatan Gigi dan Mulut. Jakarta: Pusat Data dan Informasi Kementerian Kesehatan RI; 2014.

Mount G, Hume W. Preservation and Restoration of Tooth Structure. 2nd ed. Australia: Knowledge Books and Software; 2005.

Alcorn M, Rogo E. Silver diamine fluoride. Am Dent Hyg Assoc 2012;26:18.

Libério SA, Pereira AL, Araújo MJ, Dutra RP, Nascimento FR, Monteiro-Neto V, et al. The potential use of propolis as a cariostatic agent and its actions on mutans group streptococci. J Ethnopharmacol 2009;125:1-9.

Mei ML, Ito L, Cao Y, Li QL, Lo EC, Chu CH. Inhibitory effect of silver diamine fluoride on dentine demineralisation and collagen degradation. J Dent 2013;41:809-17.

Chu CH, Mei L, Seneviratne CJ, Lo EC. Effects of silver diamine fluoride on dentine carious lesions induced by Streptococcus mutans and Actinomyces naeslundii biofilms. Int J Paediatr Dent 2012;22:2-10.

Mei Ml, Li QL, Chu CH, Lo EC, Samaranayake LP. Antibacterial effects of silver diamine fluoride on multi-species cariogenic biofilm on caries. Ann Clin Microbiol Antimicrob 2013;2:4.

Mei ML, Li QL, Chu CH, Yiu CK, Lo EC. The inhibitory effects of silver diamine fluoride at different concentrations on matrix metalloproteinases. Dent Mater 2012;28:903-8.

Knight GM, McIntyre JM, Craig GG, Mulyani, Zilm PS, Gully NJ. Inability to form a biofilm of Streptococcus mutans on silver fluoride-and potassium iodide-treated demineralized dentin. Quintessence Int 2009;40:155-61.

Rajendra A, Veitz-Keenan A, Oliveira BH, Ruff RR, Wong MC, Innes NP, et al. Topical silver diamine fluoride for managing dental caries in children and adults. Cochrane Database Syst Rev 2017;7:1-7.

Rosenblatt A, Stamford TC, Niederman R. Silver diamine fluoride: A caries “silver-fluoride bullet”. J Dent Res 2009;88:116-25.

Santos VE Jr, Filho AV, Targino AG, Flores MA, Galembeck A, Caldas AF Jr, et al. A new “silver-bullet” to treat caries in children-nano silver fluoride: A randomised clinical trial. J Dent 2014;42:945-51.

Liu BY, Lo EC, Li CM. Effect of silver and fluoride ions on enamel demineralization: A quantitative study using micro-computed tomography. Aust Dent J 2012;57:65-70.

De Luca MP, Franca JR, Augusto F, Macedo FF, Grenho L, Cortes ME, Faraco AAG. Propolis varnish: Antimicrobial properties against cariogenic bacteria, cytotoxicity, and sustained-release profile. Biomed Res Int 2014;2014:348647.

Koo H, Rosalen PL, Cury JA, Park YK, Bowen WH. Effects of compounds found in propolis on Streptococcus mutans growth and on glucosyltransferase activity. Antimicrob Agents Chemother 2002;46:1302-9.

Ghazali FB. Permeability of dentine. Malays J Med Sci 2003;10:27-36.

Zhi QH, Lo EC, Kwok AC. An in vitro study of silver and fluoride ions on remineralization of demineralized enamel and dentine. Aust Dent J 2013;58:50-6.

Elgalaid TO, Creanor SL, Creanor S, Hall AF. The permeability of natural dentine caries before and after restoration: An in vitro study. J Dent 2007;35:656-63.

Hongal S, Torwane NA, Goel P, Chandrashekar B. The effect of 30% ethanolic extract of Indian propolis on replica of human dentin compared against commercially available desensitizing agent: A methodological SEM study in vitro. Pharmacognosy Res 2014;6:113-9.

Shoham AB, Rot C, Stern T, Krief S, Akiva A, Dadosh T, et al. Deposition of collagen Type I onto skeletal endothelium reveals a new role for blood vessels in regulating bone morphology. Development 2016;143:3933-43.

Bunaciu AA, Udristioiu EG, Aboul-Enein HY. XRD: Instrumentation and applications. Crit Rev Anal Chem 2015;45:289-99.

Huang S, Zhang CP, Wang K, Li GQ, Hu FL. Recent advances in the chemical composition of propolis. Molecules 2014;19:610-32.

Menon S, Agarwal H, Kumar SR, Kumar SV. Green synthesis of silver nanoparticles using medicinal plant Acalypha indica leaf extracts and its application as an antioxidant and antimicrobial agent against foodborne pathogens. Int J Appl Pharm 2017;9:42-50.




About this article

Title

THE POTENTIAL OF SEVERAL FLUORIDE-BASED VARNISHES AS REMINERALIZATION AGENTS: MORPHOLOGICAL STUDIES, DENTIN SURFACE HARDNESS, AND CRYSTALLINITY TESTS

Keywords

Nanosilver fluoride, Propolis fluoride, Silver diamine fluoride, Remineralization, Dentin morphology, Crystallinity test, Dentine hardness

DOI

10.22159/ijap.2017.v9s2.15

Date

01-01-2018

Additional Links

Manuscript Submission

Journal

International Journal of Applied Pharmaceutics
Vol. 9, Special Issue 2 (Dec.), 2017 Page: 60-66

Online ISSN

0975-7058

Statistics

60 Views | 83 Downloads

Authors & Affiliations

Sri Angky Soekanto
Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia
Indonesia

Fadiza Fadillah
Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia

Putri Nuraisiya
Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia

Ferry Gultom
Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia

Agoeng T Sarwono
Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia


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